| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 36, 32781-32790, September 6, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Pathology, Nagoya University Graduate School
of Medicine, 65 Tsurumai-cho, Showa-ku,
Nagoya 466-8550, Japan
Using a yeast two-hybrid screen, we identified
Dok1 as a docking protein for RET tyrosine kinase. Dok1 bound more
strongly to RET with a multiple endocrine neoplasia (MEN)
2B mutation than RET with a MEN2A mutation and was highly
phosphorylated in the cells expressing the former mutant protein.
Analysis by site-directed mutagenesis revealed that tyrosine 361 in
mouse Dok1 represents a binding site for the Nck adaptor protein and
tyrosines 295, 314, 361, 376, 397, and 408 for the
Ras-GTPase-activating protein. We replaced tyrosine 361 or these six
tyrosines with phenylalanine (designated Y361F or 6F) in
Dok1 and introduced the mutant Dok1 genes into
the cells expressing the wild-type RET or RET-MEN2B protein.
Overexpression of Dok1 or Dok1-Y361F, but not Dok1-6F, suppressed the
Ras/Erk activation induced by glial cell line-derived neurotrophic
factor or RET-MEN2B, implying that this inhibitory effect requires the
Ras-GTPase-activating protein binding to Dok1. In contrast,
overexpression of Dok1, but not Dok1-Y361F or Dok1-6F, enhanced the
c-Jun amino-terminal kinase (JNK) and c-Jun activation. This suggested
that the association of Nck to tyrosine 361 in Dok1 is necessary for
the JNK and c-Jun activation by glial cell line-derived neurotrophic
factor or RET-MEN2B. Because a high level of the JNK phosphorylation
was observed in the cells expressing RET-MEN2B, its strong activation
via Nck binding to Dok1 may be responsible for aggressive
properties of medullary thyroid carcinoma developed in MEN 2B.
Role of Dok1 in Cell Signaling Mediated by RET Tyrosine
Kinase*
*
This work was supported by a grant-in-aid for Center of
Excellence research from the Ministry of Education, Science, Sports and
Culture of Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 81-52-744-2092;
Fax: 81-52-744-2098; E-mail: mtakaha@med.nagoya-u.ac.jp.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Smith, W. R. Hardy, J. M. Murphy, N. Jones, and T. Pawson Screening for PTB Domain Binding Partners and Ligand Specificity Using Proteome-Derived NPXY Peptide Arrays Mol. Cell. Biol., November 15, 2006; 26(22): 8461 - 8474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Lundgren, R. P. Scott, M. Smith, T. Pawson, and P. Ernfors Engineering the Recruitment of Phosphotyrosine Binding Domain-containing Adaptor Proteins Reveals Distinct Roles for RET Receptor-mediated Cell Survival J. Biol. Chem., October 6, 2006; 281(40): 29886 - 29896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. B. de Groot, T. P. Links, J. T. M. Plukker, C. J. M. Lips, and R. M. W. Hofstra RET as a Diagnostic and Therapeutic Target in Sporadic and Hereditary Endocrine Tumors Endocr. Rev., August 1, 2006; 27(5): 535 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Uchida, A. Enomoto, T. Fukuda, K. Kurokawa, K. Maeda, Y. Kodama, N. Asai, T. Hasegawa, Y. Shimono, M. Jijiwa, et al. Dok-4 regulates GDNF-dependent neurite outgrowth through downstream activation of Rap1 and mitogen-activated protein kinase J. Cell Sci., August 1, 2006; 119(15): 3067 - 3077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, W. Zhu, Y.-G. Wang, X.-J. Liu, L. Jiao, X. Liu, Z.-H. Zhang, C.-L. Lu, and C. He Interaction of SH2-B{beta} with RET is involved in signaling of GDNF-induced neurite outgrowth J. Cell Sci., April 15, 2006; 119(8): 1666 - 1676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Boulay, J.-G. Nemorin, and P. Duplay Phosphotyrosine Binding-Mediated Oligomerization of Downstream of Tyrosine Kinase (Dok)-1 and Dok-2 Is Involved in CD2-Induced Dok Phosphorylation J. Immunol., October 1, 2005; 175(7): 4483 - 4489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fukuda, N. Asai, A. Enomoto, and M. Takahashi Activation of c-Jun amino-terminal kinase by GDNF induces G2/M cell cycle delay linked with actin reorganization Genes Cells, July 1, 2005; 10(7): 655 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ling, L. A. Maile, J. Badley-Clarke, and D. R. Clemmons DOK1 Mediates SHP-2 Binding to the {alpha}V{beta}3 Integrin and Thereby Regulates Insulin-like Growth Factor I Signaling in Cultured Vascular Smooth Muscle Cells J. Biol. Chem., February 4, 2005; 280(5): 3151 - 3158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lee, C. Andrieu, F. Saltel, O. Destaing, J. Auclair, V. Pouchkine, J. Michelon, B. Salaun, R. Kobayashi, P. Jurdic, et al. I{kappa}B kinase {beta} phosphorylates Dok1 serines in response to TNF, IL-1, or {gamma} radiation PNAS, December 14, 2004; 101(50): 17416 - 17421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Crowder, H. Enomoto, M. Yang, E. M. Johnson Jr., and J. Milbrandt Dok-6, a Novel p62 Dok Family Member, Promotes Ret-mediated Neurite Outgrowth J. Biol. Chem., October 1, 2004; 279(40): 42072 - 42081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jijiwa, T. Fukuda, K. Kawai, A. Nakamura, K. Kurokawa, Y. Murakumo, M. Ichihara, and M. Takahashi A Targeting Mutation of Tyrosine 1062 in Ret Causes a Marked Decrease of Enteric Neurons and Renal Hypoplasia Mol. Cell. Biol., September 15, 2004; 24(18): 8026 - 8036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Nguyen, S. Kebache, A. Fazel, H. N. Wong, S. Jenna, A. Emadali, E.-h. Lee, J. J.M. Bergeron, R. J. Kaufman, L. Larose, et al. Nck-dependent Activation of Extracellular Signal-regulated Kinase-1 and Regulation of Cell Survival during Endoplasmic Reticulum Stress Mol. Biol. Cell, September 1, 2004; 15(9): 4248 - 4260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shinohara, T. Yasuda, and Y. Yamanashi Dok-1 tyrosine residues at 336 and 340 are essential for the negative regulation of Ras-Erk signalling, but dispensable for rasGAP-binding Genes Cells, June 1, 2004; 9(6): 601 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Woodring, J. Meisenhelder, S. A. Johnson, G.-L. Zhou, J. Field, K. Shah, F. Bladt, T. Pawson, M. Niki, P. P. Pandolfi, et al. c-Abl phosphorylates Dok1 to promote filopodia during cell spreading J. Cell Biol., May 24, 2004; 165(4): 493 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bedirian, C. Baldwin, J.-i. Abe, T. Takano, and S. Lemay Pleckstrin Homology and Phosphotyrosine-binding Domain-dependent Membrane Association and Tyrosine Phosphorylation of Dok-4, an Inhibitory Adapter Molecule Expressed in Epithelial Cells J. Biol. Chem., April 30, 2004; 279(18): 19335 - 19349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Castilla, P. Perrat, N. J. Martinez, S. F. Landrette, R. Keys, S. Oikemus, J. Flanegan, S. Heilman, L. Garrett, A. Dutra, et al. Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis of acute myeloid leukemia PNAS, April 6, 2004; 101(14): 4924 - 4929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shi, S. Ye, M. Bartlam, M. Yang, J. Wu, Y. Liu, F. Sun, X. Han, X. Peng, B. Qiang, et al. Structural Basis for the Specific Recognition of RET by the Dok1 Phosphotyrosine Binding Domain J. Biol. Chem., February 6, 2004; 279(6): 4962 - 4969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Woodring, T. Hunter, and J. Y. J. Wang Regulation of F-actin-dependent processes by the Abl family of tyrosine kinases J. Cell Sci., July 1, 2003; 116(13): 2613 - 2626. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
