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J. Biol. Chem., Vol. 277, Issue 36, 33075-33080, September 6, 2002
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From the Hyperosmotic stress caused by NaCl, LiCl,
or sorbitol induces an immediate and short duration (~1 min)
transient cytosolic Ca2+
([Ca2+]cyt) increase
(Ca2+-dependent aequorin luminescence) in
Saccharomyces cerevisiae cells. The amplitude of the
osmotically induced [Ca2+]cyt
transient was attenuated by the addition of chelating agents EGTA or
BAPTA, cation channel pore blockers, competitive inhibitors of
Ca2+ transport, or mutations (cch1
An Osmotically Induced Cytosolic
Ca2+ Transient Activates Calcineurin Signaling to Mediate
Ion Homeostasis and Salt Tolerance of Saccharomyces
cerevisiae*
,,
,, and**
Center for Plant Environmental Stress
Physiology, Department of Horticulture and Landscape Architecture,
Purdue University, West Lafayette, Indiana 47907-1165, the
§ Departments of Biology and Chemistry, Eastern Mennonite
University, Harrisonburg, Virginia 22802, the ¶ Department of
Chemistry, Purdue University, West Lafayette, Indiana 47907, and
Instituto de Recursos Naturales y Agrobiologia, Consejo Superior
de Investigaciones Cientificas, Sevilla 41012, Spain
or
mid1) that reduce Ca2+ influx, indicating
that Ca
mutation reduced cellular capacity for NaCl but
not hyperosmotic adaptation. The stress-adaptive effect of hyperosmotic
pretreatment was mimicked by exposing cells briefly to 20 mM CaCl2. Thus, NaCl- or sorbitol-induced
hyperosmotic shock causes a
[Ca2+]cyt transient that is
facilitated by Ca2+ influx, which enhances ionic but not
osmotic stress adaptation. NaCl-induced ENA1
expression was inhibited by EGTA, cch1 mutation, and
FK506, indicating that the
[Ca2+]cyt transient activates
calcineurin signaling to mediate ion homeostasis and salt tolerance.
*
This work was supported by National Science Foundation Plant
Genome Award DBI-9813360 (to R. A. B. and P. M. H.) and by the Thomas F. Jeffress and Kate Miller Jeffress Memorial Trust (to S. G. C.). This is journal article 16839 from the Purdue Agricultural Experiment Station.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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