JBC Advanced Glycation Endproducts

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M203834200 on June 26, 2002

J. Biol. Chem., Vol. 277, Issue 37, 33664-33669, September 13, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/37/33664    most recent
M203834200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carré, M.
Right arrow Articles by Braguer, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carré, M.
Right arrow Articles by Braguer, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Tubulin Is an Inherent Component of Mitochondrial Membranes That Interacts with the Voltage-dependent Anion Channel*

Manon CarréDagger §, Nicolas AndréDagger §, Gérard CarlesDagger , Hélène Borghi||, Laetitia Brichese**, Claudette BriandDagger , and Diane BraguerDagger Dagger Dagger

From the Dagger  UMR CNRS 6032, UFR Pharmacy and the || Department of Electron Microscopy, UFR Medicine, University of La Méditerranée, 27 Boulevard Jean Moulin, 13005 Marseille, France and ** CNRS 2079, University Paul Sabatier, 118 Route de Narbonne, 31062 Toulouse Cedex, France

We have previously reported that anti-tubulin agents induce the release of cytochrome c from isolated mitochondria. In this study, we show that tubulin is present in mitochondria isolated from different human cancerous and non-cancerous cell lines. The absence of polymerized microtubules and cytosolic proteins was checked to ensure that this tubulin is an inherent component of the mitochondria. In addition, a salt wash did not release the tubulin from the mitochondria. By using electron microscopy, we then showed that tubulin is localized in the mitochondrial membranes. As compared with cellular tubulin, mitochondrial tubulin is enriched in acetylated and tyrosinated alpha -tubulin and is also enriched in the class III beta -tubulin isotype but contains very little of the class IV beta -tubulin isotype. The mitochondrial tubulin is likely to be organized in alpha /beta dimers and represents 2.2 ± 0.5% of total cellular tubulin. Lastly, we showed by immunoprecipitation experiments that the mitochondrial tubulin is specifically associated with the voltage-dependent anion channel, the main component of the permeability transition pore. Thus, tubulin is an inherent component of mitochondrial membranes, and it could play a role in apoptosis via interaction with the permeability transition pore.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

Recipient of a fellowship from the Association pour la Recherche sur le Cancer.

Dagger Dagger To whom correspondence should be addressed. Tel.: 33-4-91-83-56-35; Fax: 33-4-91-78-20-24; E-mail: diane.braguer@pharmacie.univ.mrs.fr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. Mi, Z. Xiao, B. L. Hood, S. Dakshanamurthy, X. Wang, S. Govind, T. P. Conrads, T. D. Veenstra, and F.-L. Chung
Covalent Binding to Tubulin by Isothiocyanates: A MECHANISM OF CELL GROWTH ARREST AND APOPTOSIS
J. Biol. Chem., August 8, 2008; 283(32): 22136 - 22146.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
T. Bordet, B. Buisson, M. Michaud, J.-L. Abitbol, F. Marchand, J. Grist, E. Andriambeloson, M. Malcangio, and R. M. Pruss
Specific Antinociceptive Activity of Cholest-4-en-3-one, Oxime (TRO19622) in Experimental Models of Painful Diabetic and Chemotherapy-Induced Neuropathy
J. Pharmacol. Exp. Ther., August 1, 2008; 326(2): 623 - 632.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
L. Cicchillitti, R. Penci, M. Di Michele, F. Filippetti, D. Rotilio, M. B. Donati, G. Scambia, and C. Ferlini
Proteomic characterization of cytoskeletal and mitochondrial class III {beta}-tubulin
Mol. Cancer Ther., July 1, 2008; 7(7): 2070 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
A Stigliano, L Cerquetti, M Borro, G Gentile, B Bucci, S Misiti, P Piergrossi, E Brunetti, M Simmaco, and V Toscano
Modulation of proteomic profile in H295R adrenocortical cell line induced by mitotane
Endocr. Relat. Cancer, March 1, 2008; 15(1): 1 - 10.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Patenaude, R. G. Deschesnes, J. L.C. Rousseau, E. Petitclerc, J. Lacroix, M.-F. Cote, and R. C.-Gaudreault
New Soft Alkylating Agents with Enhanced Cytotoxicity against Cancer Cells Resistant to Chemotherapeutics and Hypoxia
Cancer Res., March 1, 2007; 67(5): 2306 - 2316.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. L. Mironov and N. Symonchuk
ER vesicles and mitochondria move and communicate at synapses
J. Cell Sci., December 1, 2006; 119(23): 4926 - 4934.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
M.-A. Esteve, M. Carre, V. Bourgarel-Rey, A. Kruczynski, G. Raspaglio, C. Ferlini, and D. Braguer
Bcl-2 down-regulation and tubulin subtype composition are involved in resistance of ovarian cancer cells to vinflunine.
Mol. Cancer Ther., November 1, 2006; 5(11): 2824 - 2833.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
I. Roman, J. Figys, G. Steurs, and M. Zizi
Hunting Interactomes of a Membrane Protein: Obtaining the Largest Set of Voltage-Dependent Anion Channel-Interacting Protein Epitopes
Mol. Cell. Proteomics, September 1, 2006; 5(9): 1667 - 1680.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
C. Siau and G. J. Bennett
Dysregulation of cellular calcium homeostasis in chemotherapy-evoked painful peripheral neuropathy.
Anesth. Analg., May 1, 2006; 102(5): 1485 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Dai, Y.-S. Lo, A. Litvinchuk, Y.-T. Wang, W.-N. Jane, L.-J. Hsiao, and K.-S. Chiang
Structural and functional characterizations of mung bean mitochondrial nucleoids
Nucleic Acids Res., August 22, 2005; 33(15): 4725 - 4739.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Ferlini, G. Raspaglio, S. Mozzetti, L. Cicchillitti, F. Filippetti, D. Gallo, C. Fattorusso, G. Campiani, and G. Scambia
The Seco-Taxane IDN5390 Is Able to Target Class III {beta}-Tubulin and to Overcome Paclitaxel Resistance
Cancer Res., March 15, 2005; 65(6): 2397 - 2405.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
K. Rezaul, L. Wu, V. Mayya, S.-I. Hwang, and D. Han
A Systematic Characterization of Mitochondrial Proteome from Human T Leukemia Cells
Mol. Cell. Proteomics, February 1, 2005; 4(2): 169 - 181.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. Mironov, M. V. Ivannikov, and M. Johansson
[Ca2+]i Signaling between Mitochondria and Endoplasmic Reticulum in Neurons Is Regulated by Microtubules: FROM MITOCHONDRIAL PERMEABILITY TRANSITION PORE TO Ca2+-INDUCED Ca2+ RELEASE
J. Biol. Chem., January 7, 2005; 280(1): 715 - 721.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
E. Pasquier, M. Carre, B. Pourroy, L. Camoin, O. Rebai, C. Briand, and D. Braguer
Antiangiogenic activity of paclitaxel is associated with its cytostatic effect, mediated by the initiation but not completion of a mitochondrial apoptotic signaling pathway
Mol. Cancer Ther., October 1, 2004; 3(10): 1301 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
B. Pourroy, M. Carre, S. Honore, V. Bourgarel-Rey, A. Kruczynski, C. Briand, and D. Braguer
Low Concentrations of Vinflunine Induce Apoptosis in Human SK-N-SH Neuroblastoma Cells through a Postmitotic G1 Arrest and a Mitochondrial Pathway
Mol. Pharmacol., September 1, 2004; 66(3): 580 - 591.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. M. Pushkarev, D. V. Starenki, V. A. Saenko, H. Namba, J. Kurebayashi, M. D. Tronko, and S. Yamashita
Molecular Mechanisms of the Effects of Low Concentrations of Taxol in Anaplastic Thyroid Cancer Cells
Endocrinology, July 1, 2004; 145(7): 3143 - 3152.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. M. Rivera, K. L. Kelley, G. W. Erdos, and P. J. Hansen
Reorganization of Microfilaments and Microtubules by Thermal Stress in Two-Cell Bovine Embryos
Biol Reprod, June 1, 2004; 70(6): 1852 - 1862.
[Abstract] [Full Text] [PDF]

Home page
 J Child NeurolHome page
C. D. Katsetos, A. Legido, E. Perentes, and S. J. Mork
Class III {beta}-Tubulin Isotype: A Key Cytoskeletal Protein at the Crossroads of Developmental Neurobiology and Tumor Neuropathology
J Child Neurol, December 1, 2003; 18(12): 851 - 866.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Shern, J. D. Sharer, D. C. Pallas, F. Bartolini, N. J. Cowan, M. S. Reed, J. Pohl, and R. A. Kahn
Cytosolic Arl2 Is Complexed with Cofactor D and Protein Phosphatase 2A
J. Biol. Chem., October 17, 2003; 278(42): 40829 - 40836.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. Ferlini, G. Raspaglio, S. Mozzetti, M. Distefano, F. Filippetti, E. Martinelli, G. Ferrandina, D. Gallo, F. O. Ranelletti, and G. Scambia
Bcl-2 Down-Regulation Is a Novel Mechanism of Paclitaxel Resistance
Mol. Pharmacol., July 1, 2003; 64(1): 51 - 58.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.