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J. Biol. Chem., Vol. 277, Issue 37, 33884-33889, September 13, 2002
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From the The trp gene encodes subunits of a
highly Ca2+-permeable class of light-activated channels of
Drosophila photoreceptors. The recently characterized
mutation in this gene, TrpP365, is semidominant
and causes massive degeneration of photoreceptors by making the TRP
channel constitutively active. We show that a single amino acid change,
Phe-550 to Ile, near the beginning of the fifth transmembrane
domain of TRP channel subunits is necessary to induce, and sufficient
to closely mimic, the original mutant phenotypes of
TrpP365. Hypotheses are presented as to
why the amino acid residues at position 550 and its immediate vicinity
might be important in influencing the regulation of the TRP channel and
why the substitution of Phe for Ile at this position, in particular,
could result in constitutive activity of the channel.
Single Amino Acid Change in the Fifth Transmembrane Segment
of the TRP Ca2+ Channel Causes Massive Degeneration of
Photoreceptors*
§,
,,,**
Department of Biological Sciences, Purdue
University, West Lafayette, Indiana 47907 and the ¶ Department
and Institute of Genetic Engineering, Kyung Hee University, Yongin
City, Kyungki-Do, 449-471, Korea
*
This work was supported by National Institutes of Health
Grant EY00033 (to W. L. P.) and by the Molecular Medicine Research Group Program from the Ministry of Science and Technology, Korea (to
J. Y.). The confocal fluorescent images were obtained in a departmental confocal facility supported by National Science Foundation Grant BIR-9512962.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: PanGen Biotech Laboratories, Inc., 802 Business Incubator Center, Kyung Hee University, Yongin City,
Kyungki-Do, 449-701, Korea.
**
To whom correspondence should be addressed: Dept. of Biological
Sciences, Purdue University, 1392 Lilly Hall, West Lafayette, IN
47907-1392. Tel.: 765-494-8202; Fax: 765-494-0876; E-mail: wpak@bilbo.bio.purdue.edu.
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