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J. Biol. Chem., Vol. 277, Issue 37, 33901-33905, September 13, 2002
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From the Department of Molecular Biology and Biochemistry,
University of California, Irvine, California 92697-3900
Sterol regulatory element-binding proteins
(SREBPs) activate promoters for key genes of metabolism to keep pace
with the cellular demand for lipids. In each SREBP-regulated
promoter, at least one ubiquitous co-regulatory factor that binds to a
neighboring recognition site is also required for efficient gene
induction. Some of these putative co-regulatory proteins are
members of transcription factor families that all bind to the same DNA
sequence elements in vitro and are often expressed in the
same cells. These two observations have made it difficult to assign
specific and redundant functions to the unique members of a specific
gene family. We have used the chromatin immunoprecipitation
(ChIP) technique coupled with a transient complementation assay in
Drosophila SL2 cells to directly compare the ability of two
members of the CREB/ATF family to function as co-regulatory proteins
for SREBP-dependent activation of the HMG-CoA reductase
promoter. Results from both of these experimental systems demonstrate
that CREB is an efficient SREBP co-regulator but ATF-2 is not.
A Role for Cyclic AMP Response Element-binding Protein (CREB) but
Not the Highly Similar ATF-2 Protein in Sterol Regulation of the
Promoter for 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase*
§,,
*
This work was supported in part by Grant HL48044 from the
National Institutes of Health and Grant 0150231N from the American Heart Association.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Both authors contributed equally to this work.
§
Recipients of undergraduate fellowships from the Undergraduate
Research Opportunities Program at the University of California, Irvine.
¶
To whom correspondence should be addressed: Dept. of Molecular
Biology and Biochemistry, University of California, Irvine, CA
92697-3900. Tel.: 949-824-2979; Fax: 949-824-8551.
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