JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M202660200 on July 15, 2002

J. Biol. Chem., Vol. 277, Issue 37, 33963-33967, September 13, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/37/33963    most recent
M202660200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jiang, Z.
Right arrow Articles by Aronson, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, Z.
Right arrow Articles by Aronson, P. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Specificity of Anion Exchange Mediated by Mouse Slc26a6*

Zhirong JiangDagger , Irina I. Grichtchenko§, Walter F. Boron§, and Peter S. AronsonDagger §

From the Departments of Dagger  Internal Medicine and of § Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520-8029

Recently, CFEX, the mouse orthologue of human SLC26A6, was localized to the brush border membrane of proximal tubule cells and was demonstrated to mediate Cl--formate exchange when expressed in Xenopus oocytes. The purpose of the present study was to examine whether mouse Slc26a6 can mediate one or more of the additional anion exchange processes observed to take place across the apical membrane of proximal tubule cells. Influx of [14C]formate into Slc26a6-expressing oocytes was inhibited by sulfate, oxalate, and p-aminohippurate (PAH), indicating affinity for these anions. Measurements of uptake of [14C]oxalate, [14C]PAH, and [35S]sulfate indicated that Slc26a6 can mediate transport of oxalate and sulfate but not PAH. Studies of the effect of external anions on [14C]oxalate efflux demonstrated Slc26a6-mediated Cl--oxalate, oxalate-formate, oxalate-oxalate, and oxalate-sulfate exchange. Two-electrode voltage clamp measurements indicated that Slc26a6-mediated Cl--oxalate exchange is electrogenic. Intracellular pH recordings demonstrated that Slc26a6 can mediate Cl--HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchange, but Cl--OH- exchange was not detected. The presence of 100 µM oxalate inhibited the rate of Cl--HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchange by 60%. We conclude that mouse Slc26a6 has affinity for oxalate, sulfate, and HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> in addition to Cl- and formate and can function in multiple exchange modes involving pairs of these anions. In the presence of high oxalate concentrations as found in renal tubular fluid and urine, Slc26a6 may largely function as an electrogenic Cl--oxalate exchanger.

* This work was supported by National Institutes of Health Grants DK-33793 and DK-17433.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Section of Nephrology, Dept. of Internal Medicine, Yale University School of Medicine, 333 Cedar St., LMP 2073, New Haven, CT 06520-8029. Tel.: 203-785-4186; Fax: 203-785-7068; E-mail: peter.aronson@yale.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
N. Shcheynikov, D. Yang, Y. Wang, W. Zeng, L. P. Karniski, I. So, S. M. Wall, and S. Muallem
The Slc26a4 transporter functions as an electroneutral Cl-/I-/HCO3- exchanger: role of Slc26a4 and Slc26a6 in I- and HCO3- secretion and in regulation of CFTR in the parotid duct
J. Physiol., August 15, 2008; 586(16): 3813 - 3824.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
M. R. Dorwart, N. Shcheynikov, D. Yang, and S. Muallem
The Solute Carrier 26 Family of Proteins in Epithelial Ion Transport
Physiology, April 1, 2008; 23(2): 104 - 114.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. R. Dorwart, N. Shcheynikov, J. M. R. Baker, J. D. Forman-Kay, S. Muallem, and P. J. Thomas
Congenital Chloride-losing Diarrhea Causing Mutations in the STAS Domain Result in Misfolding and Mistrafficking of SLC26A3
J. Biol. Chem., March 28, 2008; 283(13): 8711 - 8722.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. S. Clark, D. H. Vandorpe, M. N. Chernova, J. F. Heneghan, A. K. Stewart, and S. L. Alper
Species differences in Cl- affinity and in electrogenicity of SLC26A6-mediated oxalate/Cl- exchange correlate with the distinct human and mouse susceptibilities to nephrolithiasis
J. Physiol., March 1, 2008; 586(5): 1291 - 1306.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Detro-Dassen, M. Schanzler, H. Lauks, I. Martin, S. M. z. Berstenhorst, D. Nothmann, D. Torres-Salazar, P. Hidalgo, G. Schmalzing, and C. Fahlke
Conserved Dimeric Subunit Stoichiometry of SLC26 Multifunctional Anion Exchangers
J. Biol. Chem., February 15, 2008; 283(7): 4177 - 4188.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. J. Schaechinger and D. Oliver
Nonmammalian orthologs of prestin (SLC26A5) are electrogenic divalent/chloride anion exchangers
PNAS, May 1, 2007; 104(18): 7693 - 7698.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Simpson, C. W. Schweinfest, G. E. Shull, L. R. Gawenis, N. M. Walker, K. T. Boyle, M. Soleimani, and L. L. Clarke
PAT-1 (Slc26a6) is the predominant apical membrane Cl-/HCO3- exchanger in the upper villous epithelium of the murine duodenum
Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1079 - G1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. A. Hassan, S. Mentone, L. P. Karniski, V. M. Rajendran, and P. S. Aronson
Regulation of anion exchanger Slc26a6 by protein kinase C
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1485 - C1492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. W. Schweinfest, D. D. Spyropoulos, K. W. Henderson, J.-H. Kim, J. M. Chapman, S. Barone, R. T. Worrell, Z. Wang, and M. Soleimani
slc26a3 (dra)-deficient Mice Display Chloride-losing Diarrhea, Enhanced Colonic Proliferation, and Distinct Up-regulation of Ion Transporters in the Colon
J. Biol. Chem., December 8, 2006; 281(49): 37962 - 37971.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. Muallem and J. Ashmore
An Anion Antiporter Model of Prestin, the Outer Hair Cell Motor Protein
Biophys. J., June 1, 2006; 90(11): 4035 - 4045.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Katoh, M. Tresguerres, K. M. Lee, T. Kaneko, K. Aida, and G. G. Goss
Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1468 - R1478.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
N. Shcheynikov, Y. Wang, M. Park, S. B.H. Ko, M. Dorwart, S. Naruse, P. J. Thomas, and S. Muallem
Coupling Modes and Stoichiometry of Cl-/HCO3- Exchange by slc26a3 and slc26a6
J. Gen. Physiol., April 24, 2006; 127(5): 511 - 524.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. W. Freel, M. Hatch, M. Green, and M. Soleimani
Ileal oxalate absorption and urinary oxalate excretion are enhanced in Slc26a6 null mice
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G719 - G728.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. L. Dudas, S. Mentone, C. F. Greineder, D. Biemesderfer, and P. S. Aronson
Immunolocalization of anion transporter Slc26a7 in mouse kidney
Am J Physiol Renal Physiol, April 1, 2006; 290(4): F937 - F945.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. T. Worrell, A. Best, O. R. Crawford, J. Xu, M. Soleimani, and J. B. Matthews
Apical ammonium inhibition of cAMP-stimulated secretion in T84 cells is bicarbonate dependent
Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G768 - G778.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. B. Thomson, T. Wang, B. R. Thomson, L. Tarrats, A. Girardi, S. Mentone, M. Soleimani, O. Kocher, and P. S. Aronson
Role of PDZK1 in membrane expression of renal brush border ion exchangers
PNAS, September 13, 2005; 102(37): 13331 - 13336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. E. Simpson, L. R. Gawenis, N. M. Walker, K. T. Boyle, and L. L. Clarke
Chloride conductance of CFTR facilitates basal Cl-/HCO3- exchange in the villous epithelium of intact murine duodenum
Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1241 - G1251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Wang, T. Wang, S. Petrovic, B. Tuo, B. Riederer, S. Barone, J. N. Lorenz, U. Seidler, P. S. Aronson, and M. Soleimani
Renal and intestinal transport defects in Slc26a6-null mice
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C957 - C965.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. N. Chernova, L. Jiang, D. J. Friedman, R. B. Darman, H. Lohi, J. Kere, D. H. Vandorpe, and S. L. Alper
Functional Comparison of Mouse slc26a6 Anion Exchanger with Human SLC26A6 Polypeptide Variants: DIFFERENCES IN ANION SELECTIVITY, REGULATION, AND ELECTROGENICITY
J. Biol. Chem., March 4, 2005; 280(9): 8564 - 8580.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. M. Pelis and J. L. Renfro
Role of tubular secretion and carbonic anhydrase in vertebrate renal sulfate excretion
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R491 - R501.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Barone, H. Amlal, J. Xu, M. Kujala, J. Kere, S. Petrovic, and M. Soleimani
Differential Regulation of Basolateral Cl-/HCO3- Exchangers SLC26A7 and AE1 in Kidney Outer Medullary Collecting Duct
J. Am. Soc. Nephrol., August 1, 2004; 15(8): 2002 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Petrovic, S. Barone, A. M. Weinstein, and M. Soleimani
Activation of the apical Na+/H+ exchanger NHE3 by formate: a basis of enhanced fluid and electrolyte reabsorption by formate in the kidney
Am J Physiol Renal Physiol, August 1, 2004; 287(2): F336 - F346.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. R. Gawenis, C. Ledoussal, L. M. Judd, V. Prasad, S. L. Alper, A. Stuart-Tilley, A. L. Woo, C. Grisham, L. P. Sanford, T. Doetschman, et al.
Mice with a Targeted Disruption of the AE2 Cl-/HCO3- Exchanger Are Achlorhydric
J. Biol. Chem., July 16, 2004; 279(29): 30531 - 30539.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Pedrosa, P. A. Jose, and P. Soares-da-Silva
Defective D1-like receptor-mediated inhibition of the Cl-/HCO3- exchanger in immortalized SHR proximal tubular epithelial cells
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1120 - F1126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. W. Beyenbach
Kidneys sans glomeruli
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F811 - F827.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Petrovic, S. Barone, J. Xu, L. Conforti, L. Ma, M. Kujala, J. Kere, and M. Soleimani
SLC26A7: a basolateral Cl-/HCO3- exchanger specific to intercalated cells of the outer medullary collecting duct
Am J Physiol Renal Physiol, January 1, 2004; 286(1): F161 - F169.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Petrovic, L. Ma, Z. Wang, and M. Soleimani
Identification of an apical Cl-/HCO-3 exchanger in rat kidney proximal tubule
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C608 - C617.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Petrovic, X. Ju, S. Barone, U. Seidler, S. L. Alper, H. Lohi, J. Kere, and M. Soleimani
Identification of a basolateral Cl-/HCO3- exchanger specific to gastric parietal cells
Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1093 - G1103.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Lohi, G. Lamprecht, D. Markovich, A. Heil, M. Kujala, U. Seidler, and J. Kere
Isoforms of SLC26A6 mediate anion transport and have functional PDZ interaction domains
Am J Physiol Cell Physiol, March 1, 2003; 284(3): C769 - C779.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.