|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 37, 34271-34279, September 13, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biochemistry, University of Connecticut
Health Center, Farmington, Connecticut 06030-3305
Regulation of flagellar activity in
Chlamydomonas involves both Ca2+ and
cAMP-mediated signaling pathways. However, Chlamydomonas and sea urchin sperm flagella also exhibit nucleoside-diphosphate kinase (NDK) activity, suggesting a requirement for GTP within this
highly conserved organelle. In sea urchin sperm, the NDK catalytic
subunit is an integral component of the outer dynein arm. Here we
describe a modular protein (p72) from the Chlamydomonas flagellum that consists of three domains closely related to the presumptive regulatory segment of rat NDK-7 followed by two EF-hands that are predicted to bind Ca2+. There are close homologues
of p72 in both mammalian and insect genomes. The p72 protein is tightly
associated with the flagellar axoneme and is located along the entire
length except at the transition zone. Cross-linking experiments suggest
that p72 interacts with two or three additional axonemal polypeptides.
The sensitivity of p72 to tryptic digestion differed considerably in
the presence and the absence of Ca2+, suggesting that it
indeed binds this ligand. These studies indicate that the flagellar NDK
system is bipartite with the regulatory and catalytic components
residing on different polypeptides. We propose that Ca2+
regulation of flagellar motility in Chlamydomonas may be
achieved in part through a downstream GTP-mediated signaling pathway.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) BK000460.
A Bipartite Ca2+-regulated
Nucleoside-diphosphate Kinase System within the
Chlamydomonas Flagellum
THE REGULATORY SUBUNIT p72*
*
This work was supported by Grant GM51293 from the National
Institutes of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Investigator of the Patrick and Catherine Weldon Donaghue Medical
Research Foundation. To whom correspondence should be addressed: Dept.
of Biochemistry, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3305. Tel.: 860-679-3347; Fax: 860-679-3408; Email: steve@king2.uchc.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Duriez, P. Duquesnoy, E. Escudier, A.-M. Bridoux, D. Escalier, I. Rayet, E. Marcos, A.-M. Vojtek, J.-F. Bercher, and S. Amselem A common variant in combination with a nonsense mutation in a member of the thioredoxin family causes primary ciliary dyskinesia PNAS, February 27, 2007; 104(9): 3336 - 3341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kinukawa, M. Nomura, and V. D. Vacquier A Sea Urchin Sperm Flagellar Adenylate Kinase with Triplicated Catalytic Domains J. Biol. Chem., February 2, 2007; 282(5): 2947 - 2955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Baron, K. S. Ralston, Z. P. Kabututu, and K. L. Hill Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella J. Cell Sci., February 1, 2007; 120(3): 478 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Okita, N. Isogai, M. Hirono, R. Kamiya, and K. Yoshimura Phototactic activity in Chlamydomonas 'non-phototactic' mutants deficient in Ca2+-dependent control of flagellar dominance or in inner-arm dynein J. Cell Sci., February 1, 2005; 118(3): 529 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Patel-King, O. Gorbatyuk, S. Takebe, and S. M. King Flagellar Radial Spokes Contain a Ca2+-stimulated Nucleoside Diphosphate Kinase Mol. Biol. Cell, August 1, 2004; 15(8): 3891 - 3902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Pullen, M. L. Ginger, S. J. Gaskell, and K. Gull Protein Targeting of an Unusual, Evolutionarily Conserved Adenylate Kinase to a Eukaryotic Flagellum Mol. Biol. Cell, July 1, 2004; 15(7): 3257 - 3265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Wargo, M. A. McPeek, and E. F. Smith Analysis of microtubule sliding patterns in Chlamydomonas flagellar axonemes reveals dynein activity on specific doublet microtubules J. Cell Sci., May 15, 2004; 117(12): 2533 - 2544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Sadek, A. Jimenez, A. E. Damdimopoulos, T. Kieselbach, M. Nord, J.-A. Gustafsson, G. Spyrou, E. C. Davis, R. Oko, F. A. van der Hoorn, et al. Characterization of Human Thioredoxin-like 2. A NOVEL MICROTUBULE-BINDING THIOREDOXIN EXPRESSED PREDOMINANTLY IN THE CILIA OF LUNG AIRWAY EPITHELIUM AND SPERMATID MANCHETTE AND AXONEME J. Biol. Chem., April 4, 2003; 278(15): 13133 - 13142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ikeda, J. A. Brown, T. Yagi, J. M. Norrander, M. Hirono, E. Eccleston, R. Kamiya, and R. W. Linck Rib72, a Conserved Protein Associated with the Ribbon Compartment of Flagellar A-microtubules and Potentially Involved in the Linkage between Outer Doublet Microtubules J. Biol. Chem., February 21, 2003; 278(9): 7725 - 7734. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |