HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 38, 34846-34852, September 20, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/38/34846    most recent M202902200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Duggan, M. J. Articles by Foster, K. A. Search for Related Content PubMed PubMed Citation Articles by Duggan, M. J. Articles by Foster, K. A. Social Bookmarking                      What's this?

Inhibition of Release of Neurotransmitters from Rat Dorsal Root Ganglia by a Novel Conjugate of a Clostridium botulinum Toxin A Endopeptidase Fragment and Erythrina cristagalli Lectin^*

Michael J. Duggan^§, Conrad P. Quinn^¶, John A. Chaddock, John R. Purkiss, Frances C. G. Alexander, Sarah Doward, Sarah J. Fooks, Lorna M. Friis, Yper H. J. Hall, Elizabeth R. Kirby, Nicola Leeds, Hilary J. Moulsdale, Anthony Dickenson^**, G. Mark Green^**, Wahida Rahman^**, Rie Suzuki^**, Clifford C. Shone, and Keith A. Foster

From the  Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, United Kingdom and the ^** University College London, University College, Gower Street, London WC1E 6BT, United Kingdom

Clostridial neurotoxins potently and specifically inhibit neurotransmitter release in defined cell types. Here we report that a catalytically active derivative (termed LH_N/A) of the type A neurotoxin from Clostridium botulinum has been coupled to a lectin obtained from Erythrina cristagalli to form a novel conjugate. This conjugate exhibits an in vitro selectivity for nociceptive afferents compared with the anatomically adjacent spinal neurons, as assessed using in vitro primary neuronal culture systems to measure inhibition of release of neurotransmitters. Chemical conjugates prepared between E. cristagalli lectin and either natively sourced LH_N/A or recombinant LH_N/A purified from Escherichia coli are assessed, and equivalence of the recombinant material are demonstrated. Furthermore, the dependence of inhibition of neurotransmitter release on the cleavage of SNAP-25 is demonstrated through the use of an endopeptidase-deficient LH_N/A conjugate variant. The duration of action of inhibition of neurotransmitter released by the conjugate in vitro is assessed and is comparable with that observed with Clostridium botulinum neurotoxin. Finally, in vivo electrophysiology shows that these in vitro actions have biological relevance in that sensory transmission from nociceptive afferents through the spinal cord is significantly attenuated. These data demonstrate that the potent endopeptidase activity of clostridial neurotoxins can be selectively retargeted to cells of interest and that inhibition of release of neurotransmitters from a neuronal population of therapeutic relevance to the treatment of pain can be achieved.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. Chen and J. T. Barbieri From the Cover: Engineering botulinum neurotoxin to extend therapeutic intervention PNAS, June 9, 2009; 106(23): 9180 - 9184. [Abstract] [Full Text] [PDF]

				K. Turton, R. Natesh, N. Thiyagarajan, J. A. Chaddock, and K. R. Acharya Crystal structures of Erythrina cristagalli lectin with bound N-linked oligosaccharide and lactose Glycobiology, October 1, 2004; 14(10): 923 - 929. [Abstract] [Full Text] [PDF]

				V P. Misra The changed image of botulinum toxin BMJ, November 23, 2002; 325(7374): 1188 - 1188. [Full Text] [PDF]