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J. Biol. Chem., Vol. 277, Issue 38, 35176-35182, September 20, 2002
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From the LBCMCP-CNRS UMR5088, Université Paul Sabatier, 118 route de Narbonne, 31077 Toulouse, France
CDC25B phosphatases are essential regulators that
control cyclin-dependent kinases activities at the entry
into mitosis. In this study, we demonstrate that serine 146 is required
for two crucial features of CDC25B1. It is essential for CDC25B1 to
function as a mitotic inducer and to prevent CDC25B1 export from the
nucleus. We also show that serine 146 is phosphorylated in
vitro by CDK1-cyclin B. However, phosphorylation of CDC25B does
not stimulate its phosphatase activity, and mutation of serine 146 had
no effect on its catalytic activity. Serine 146 phosphorylation is
proposed to be a key event in the regulation of the CDC25B function in
the initiation of mammalian mitosis.
Nuclear Localization of CDC25B1 and Serine 146 Integrity Are
Required for Induction of Mitosis*
,
*
This work was initiated with support from l'Association
pour la Recherche contre le Cancer and la Fondation pour la Recherche Médicale and is currently funded by la Ligue Nationale Contre le
Cancer.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
33-5-61-55-81-10; Fax: 33-5-61-55-81-09; E-mail: ducommun@cict.fr.
§
Recipient of a postdoctoral fellowship from the Société
de Secours des Amis des Sciences.
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