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J. Biol. Chem., Vol. 277, Issue 38, 35248-35256, September 20, 2002
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From the The molecular mechanisms of cold acclimation are
still largely unknown; however, it has been established that
overwintering plants such as winter wheat increases freeze tolerance
during cold treatments. In prokaryotes, cold shock proteins are induced by temperature downshifts and have been proposed to function as RNA
chaperones. A wheat cDNA encoding a putative nucleic acid-binding protein, WCSP1, was isolated and found to be homologous to the predominant CspA of Escherichia coli. The putative WCSP1
protein contains a three-domain structure consisting of an N-terminal cold shock domain with two internal conserved consensus RNA binding domains and an internal glycine-rich region, which is interspersed with
three C-terminal
CX2CX4HX4C
(CCHC) zinc fingers. Each domain has been described
independently within several nucleotide-binding proteins.
Northern and Western blot analyses showed that WCSP1 mRNA and protein levels steadily increased during cold acclimation, respectively. WCSP1 induction was cold-specific because
neither abscisic acid treatment, drought, salinity, nor heat stress
induced WCSP1 expression. Nucleotide binding assays
determined that WCSP1 binds ssDNA, dsDNA, and RNA homopolymers. The
capacity to bind dsDNA was nearly eliminated in a mutant protein
lacking C-terminal zinc fingers. Structural and expression
similarities to E. coli CspA suggest that WCSP1 may be
involved in gene regulation during cold acclimation.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AB066265.
A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat
Shares a Domain with Bacterial Cold Shock Proteins*
§¶,§
,**, and
Winter Stress Laboratory, National
Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan, the United Graduate School
of Agricultural Science, Iwate University, Morioka 020-8550, Japan,
and the ** Department of Bioresources, Yamagata University,
Tsuruoka 997-8555, Japan
*
This work was supported by Grant 1207 of the Biodesign
Project from the Ministry of Agriculture, Forestry, and Fisheries (to R. I.) and by the Cooperative System for Supporting Priority Research, Japan Science and Technology Cooperation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
81-11-857-9382; E-mail: rzi@affrc.go.jp.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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