HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 38, 35371-35377, September 20, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/38/35371    most recent M205143200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Santos, M. Articles by Jorcano, J. L. Search for Related Content PubMed PubMed Citation Articles by Santos, M. Articles by Jorcano, J. L. Social Bookmarking                      What's this?

Severe Abnormalities in the Oral Mucosa Induced by Suprabasal Expression of Epidermal Keratin K10 in Transgenic Mice^*

Mirentxu Santos, Ana Bravo^§, Ceferino López^§, Jesús M. Paramio^¶, and José L. Jorcano

From the  Project on Cell and Molecular Biology and Gene Therapy, CIEMAT Av. Complutense 22, E-28040 Madrid, Spain and the ^§ Department of Animal Pathology, Veterinary School, University of Santiago de Compostela, 27002 Lugo, Spain

Previous studies have demonstrated that keratin K10 plays an important role in mediating cell signaling processes, since the ectopic expression of this keratin induces cell cycle arrest in proliferating cells in vitro and in vivo. However, apart from its well known function of providing epithelial cells with resilience to mechanical trauma, little is known about its possible roles in nondividing cells. To investigate what these might be, transgenic mice were generated in which the expression of K10 was driven by bovine K6 gene control elements (bK6hK10). The transgenic mice displayed severe abnormalities in the tongue and palate but not in other K6-expressing cells such as those of the esophagus, nails, and hair follicles. The lesions in the tongue and palate included the cytolysis of epithelial suprabasal cells associated with an acute inflammatory response and lymphocyte infiltration. The alterations in the oral mucosa caused the death of transgenic pups soon after birth, probably because suckling was impaired. These anomalies, together with others found in the teeth, are reminiscent of the lesions observed in some patients with pachyonychia congenita, an inherited epithelial fragility associated with mutations in keratins K6 and K16. Although no epithelial fragility was observed in the bK6hK10 oral epithelia of the experimental mice, necrotic processes were seen. Collectively, these data show that the carefully regulated tissue- and differentiation-specific patterns displayed by the keratin genes have dramatic consequences on the biological behavior of epithelial cells and that changes in the specific composition of the keratin intermediate filament cytoskeleton can affect their physiology, in particular those of the oral mucosa.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				G. Fitsialos, A.-A. Chassot, L. Turchi, M. A. Dayem, K. LeBrigand, C. Moreilhon, G. Meneguzzi, R. Busca, B. Mari, P. Barbry, et al. Transcriptional Signature of Epidermal Keratinocytes Subjected to in Vitro Scratch Wounding Reveals Selective Roles for ERK1/2, p38, and Phosphatidylinositol 3-Kinase Signaling Pathways J. Biol. Chem., May 18, 2007; 282(20): 15090 - 15102. [Abstract] [Full Text] [PDF]

				B. K. Bloor, N. Tidman, I. M. Leigh, E. Odell, B. Dogan, U. Wollina, L. Ghali, and A. Waseem Expression of Keratin K2e in Cutaneous and Oral Lesions: Association with Keratinocyte Activation, Proliferation, and Keratinization Am. J. Pathol., March 1, 2003; 162(3): 963 - 975. [Abstract] [Full Text] [PDF]