JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M110917200 on July 8, 2002

J. Biol. Chem., Vol. 277, Issue 38, 35496-35502, September 20, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/38/35496    most recent
M110917200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, D. D. H.
Right arrow Articles by Groblewski, G. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, D. D. H.
Right arrow Articles by Groblewski, G. E.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification of Annexin VI as a Ca2+-sensitive CRHSP-28-binding Protein in Pancreatic Acinar Cells*

Diana D. H. Thomas, Kala M. Kaspar, William B. Taft, Ning Weng, Lance A. Rodenkirch, and Guy E. GroblewskiDagger

From the Department of Nutritional Sciences, University of Wisconsin, Madison, Wisconsin 53706

CRHSP-28 is a member of the tumor protein D52 protein family that was recently shown to regulate Ca2+-stimulated secretory activity in streptolysin-O-permeabilized acinar cells (Thomas, D. H., Taft, W. B., Kaspar, K. M., and Groblewski, G. E. (2001) J. Biol. Chem. 276, 28866-28872). In the present study, the Ca2+-sensitive phospholipid-binding protein annexin VI was purified from rat pancreas as a CRHSP-28-binding protein. The interaction between CRHSP-28 and annexin VI was demonstrated by coimmunoprecipitation and gel-overlay assays and was shown to require low micromolar levels of free Ca2+, indicating these molecules likely interact under physiological conditions. Immunofluorescence microscopy confirmed a dual localization of CRHSP-28 and annexin VI, which appeared in a punctate pattern in the supranuclear and apical cytoplasm of acini. Stimulation of cells for 5 min with the secretagogue cholecystokinin enhanced the colocalization of CRHSP-28 and annexin VI within regions of acini immediately below the apical plasma membrane. Tissue fractionation revealed that CRHSP-28 is a peripheral membrane protein that is highly enriched in smooth microsomal fractions of pancreas. Further, the content of CRHSP-28 in microsomes was significantly reduced in pancreatic tissue obtained from rats that had been infused with a secretory dose of cholecystokinin for 40 min, demonstrating that secretagogue stimulation transiently alters the association of CRHSP-28 with membranes in cells. Collectively, the Ca2+-dependent binding of CRHSP-28 and annexin VI, together with their colocalization in the apical cytoplasm, is consistent with a role for these molecules in acinar cell membrane trafficking events that are essential for digestive enzyme secretion.


* This work was supported by Grants WISO4221 and WIS04444 from the United States Department of Agriculture Cooperative State Research Education and Extension Service Program, by American Cancer Society Institutional Research Grant IRG-58-011-42-2, and by National Science Foundation Award MCB-0094154 (to G. E. G.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Nutritional Sciences, University of Wisconsin, 1415 Linden Dr., Madison, WI 53706. Tel.: 608-262-0884; Fax: 608-262-5860; E-mail: groby@ nutrisci.wisc.edu.


Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. S. Chew, X. Chen, H. Zhang, E. A. Berg, and H. Zhang
Calcium/calmodulin-dependent phosphorylation of tumor protein D52 on serine residue 136 may be mediated by CAMK2{delta}6
Am J Physiol Gastrointest Liver Physiol, December 1, 2008; 295(6): G1159 - G1172.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
G. Bode, A. Luken, C. Kerkhoff, J. Roth, S. Ludwig, and W. Nacken
Interaction between S100A8/A9 and Annexin A6 Is Involved in the Calcium-induced Cell Surface Exposition of S100A8/A9
J. Biol. Chem., November 14, 2008; 283(46): 31776 - 31784.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
M. Shehata, I. Bieche, R. Boutros, J. Weidenhofer, S. Fanayan, L. Spalding, N. Zeps, K. Byth, R. K. Bright, R. Lidereau, et al.
Nonredundant Functions for Tumor Protein D52-Like Proteins Support Specific Targeting of TPD52
Clin. Cancer Res., August 15, 2008; 14(16): 5050 - 5060.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
R. Wang, J. Xu, N. Mabjeesh, G. Zhu, J. Zhou, M. Amin, D. He, F. F. Marshall, H. E. Zhau, and L. W.K. Chung
PrLZ Is Expressed in Normal Prostate Development and in Human Prostate Cancer Progression
Clin. Cancer Res., October 15, 2007; 13(20): 6040 - 6048.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
H. Zhang, J. Wang, B. Pang, R.-x. Liang, S. Li, P.-t. Huang, R. Wang, L. W.K. Chung, H. E. Zhau, C. Huang, et al.
PC-1/PrLZ Contributes to Malignant Progression in Prostate Cancer
Cancer Res., September 15, 2007; 67(18): 8906 - 8913.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. Weng, D. D. H. Thomas, and G. E. Groblewski
Pancreatic Acinar Cells Express Vesicle-associated Membrane Protein 2- and 8-Specific Populations of Zymogen Granules with Distinct and Overlapping Roles in Secretion
J. Biol. Chem., March 30, 2007; 282(13): 9635 - 9645.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
E. Tiacci, P.-L. Orvietani, B. Bigerna, A. Pucciarini, G. L. Corthals, V. Pettirossi, M. P. Martelli, A. Liso, R. Benedetti, R. Pacini, et al.
Tumor protein D52 (TPD52): a novel B-cell/plasma-cell molecule with unique expression pattern and Ca2+-dependent association with annexin VI
Blood, April 1, 2005; 105(7): 2812 - 2820.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. D. H. Thomas, N. Weng, and G. E. Groblewski
Secretagogue-induced translocation of CRHSP-28 within an early apical endosomal compartment in acinar cells
Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G253 - G263.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Cho, H.-M. Ko, J.-M. Kim, J.-A Lee, J.-E. Park, M.-S. Jang, S. G. Park, D. H. Lee, S.-E. Ryu, and B.-C. Park
Positive Regulation of Apoptosis Signal-regulating Kinase 1 by hD53L1
J. Biol. Chem., April 16, 2004; 279(16): 16050 - 16056.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
R. Wang, J. Xu, O. Saramaki, T. Visakorpi, W. M. Sutherland, J. Zhou, B. Sen, S. D. Lim, N. Mabjeesh, M. Amin, et al.
PrLZ, a Novel Prostate-Specific and Androgen-Responsive Gene of the TPD52 Family, Amplified in Chromosome 8q21.1 and Overexpressed in Human Prostate Cancer
Cancer Res., March 1, 2004; 64(5): 1589 - 1594.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. M. Kaspar, D. D. H. Thomas, W. B. Taft, E. Takeshita, N. Weng, and G. E. Groblewski
CaM kinase II regulation of CRHSP-28 phosphorylation in cultured mucosal T84 cells
Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1300 - G1309.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.