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Originally published In Press as doi:10.1074/jbc.M202897200 on July 17, 2002

J. Biol. Chem., Vol. 277, Issue 39, 36853-36862, September 27, 2002
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The Influence of the Cdc27 Subunit on the Properties of the Schizosaccharomyces pombe DNA Polymerase delta *

Vladimir P. Bermudez, Stuart A. MacNeillDagger , Inger Tappin, and Jerard Hurwitz§

From the Memorial Sloan-Kettering Cancer Center, Program of Molecular Biology, New York, New York 10021 and Dagger  Wellcome Trust Center for Cell Biology, University of Edinburgh, Edinburgh, Scotland, United Kingdom EH93JR

Schizosaccharomyces pombe DNA polymerase (pol) delta  contains four subunits, pol 3, Cdc1, Cdc27, and Cdm1. In this report, we examined the role of Cdc27 on the structure and activity of pol delta . We show that the four-subunit complex is monomeric in structure, in contrast to the previous report that it was a dimer (Zuo, S., Bermudez, V., Zhang, G., Kelman, Z., and Hurwitz, J. (2000) J. Biol. Chem. 275, 5153-5162). This discrepancy between the earlier and recent observations was traced to the marked asymmetric shape of Cdc27. Cdc27 contains two critical domains that govern its role in activating pol delta . The N-terminal region (amino acids (aa) 1-160) binds to Cdc1 and its extreme C-terminal end (aa 362-369) interacts with proliferating cell nuclear antigen (PCNA). Mutants of S. pombe pol delta , containing truncated Cdc27 derivatives deficient in binding to PCNA, supported DNA replication less processively than the wild-type complex. Fusion of a minimal PCNA-binding motif (aa 352-372) to C-terminally truncated Cdc27 derivatives restored processive DNA synthesis in vitro. In vivo, the introduction of these fused Cdc27 derivatives into cdc27Delta cells conferred viability. These data support the model in which Cdc27 plays an essential role in DNA replication by recruiting PCNA to the pol delta  holoenzyme.

* This work was supported in part by National Institutes of Health Grant GM 58559 (to J. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Professor of the American Cancer Society. To whom correspondence should be addressed. Tel.: 212-639-5896; Fax: 212-717-3627; E-mail: j-hurwitz@ski.mskcc.org.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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