HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 4, 2385-2395, January 25, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/4/2385    most recent M107530200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rauscher, L. Articles by Trautwein, A. X. Search for Related Content PubMed PubMed Citation Articles by Rauscher, L. Articles by Trautwein, A. X. Social Bookmarking                      What's this?

Chrysobactin-dependent Iron Acquisition in Erwinia chrysanthemi
FUNCTIONAL STUDY OF A HOMOLOG OF THE ESCHERICHIA COLI FERRIC ENTEROBACTIN ESTERASE^*

Lise Rauscher, Dominique Expert^§, Berthold F. Matzanke^¶, and Alfred X. Trautwein^¶**

From the  Laboratoire de Pathologie Végétale, UMR 217 Institut National de la Recherche Agronomique, Institut National Agronomique Paris-Grignon, Université Paris 6, 16 rue Claude Bernard, 75231 Paris Cedex 05, France and the ^¶ Medical University Lübeck, the  Institute of Physics and the ^** Isotope Laboratory, Ratzeburger Alle 160, D-23538 Lübeck, Germany

Under iron limitation, the plant pathogen Erwinia chrysanthemi produces the catechol-type siderophore chrysobactin, which acts as a virulence factor. It can also use enterobactin as a xenosiderophore. We began this work by sequencing the 5'-upstream region of the fct-cbsCEBA operon, which encodes the ferric chrysobactin receptor and proteins involved in synthesis of the catechol moiety. We identified a new iron-regulated gene (cbsH) transcribed divergently relative to the fct gene, the translated sequence of which is 45.6% identical to that of Escherichia coli ferric enterobactin esterase. Insertions within this gene interrupt the chrysobactin biosynthetic pathway by exerting a polar effect on a downstream gene with some sequence identity to the E. coli enterobactin synthase gene. These mutations had no effect on the ability of the bacterium to obtain iron from enterobactin, showing that a functional cbsH gene is not required for iron removal from ferric enterobactin in E. chrysanthemi. The cbsH-negative mutants were less able to utilize ferric chrysobactin, and this effect was not caused by a defect in transport per se. In a nonpolar cbsH-negative mutant, chrysobactin accumulated intracellularly. These defects were rescued by the cbsH gene supplied on a plasmid. The amino acid sequence of the CbsH protein revealed characteristics of the S9 prolyl oligopeptidase family. Ferric chrysobactin hydrolysis was detected in cell extracts from a cbsH-positive strain that was inhibited by diisopropyl fluorophosphate. These data are consistent with the fact that chrysobactin is a D-lysyl-L-serine derivative. Mössbauer spectroscopy of whole cells at various states of ⁵⁷Fe-labeled chrysobactin uptake showed that this enzyme is not required for iron removal from chrysobactin in vivo. The CbsH protein may therefore be regarded as a peptidase that prevents the bacterial cells from being intracellularly iron-depleted by chrysobactin.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AF011334.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. Expert, A. Boughammoura, and T. Franza Siderophore-controlled Iron Assimilation in the Enterobacterium Erwinia chrysanthemi: EVIDENCE FOR THE INVOLVEMENT OF BACTERIOFERRITIN AND THE Suf IRON-SULFUR CLUSTER ASSEMBLY MACHINERY J. Biol. Chem., December 26, 2008; 283(52): 36564 - 36572. [Abstract] [Full Text] [PDF]

				A. Boughammoura, B. F. Matzanke, L. Bottger, S. Reverchon, E. Lesuisse, D. Expert, and T. Franza Differential Role of Ferritins in Iron Metabolism and Virulence of the Plant-Pathogenic Bacterium Erwinia chrysanthemi 3937 J. Bacteriol., March 1, 2008; 190(5): 1518 - 1530. [Abstract] [Full Text] [PDF]

				M. Miethke and M. A. Marahiel Siderophore-Based Iron Acquisition and Pathogen Control Microbiol. Mol. Biol. Rev., September 1, 2007; 71(3): 413 - 451. [Abstract] [Full Text] [PDF]

				C. Kragl, M. Schrettl, B. Abt, B. Sarg, H. H. Lindner, and H. Haas EstB-Mediated Hydrolysis of the Siderophore Triacetylfusarinine C Optimizes Iron Uptake of Aspergillus fumigatus Eukaryot. Cell, August 1, 2007; 6(8): 1278 - 1285. [Abstract] [Full Text] [PDF]

				M. Balado, C. R. Osorio, and M. L. Lemos A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produced by Vibrio anguillarum Microbiology, December 1, 2006; 152(12): 3517 - 3528. [Abstract] [Full Text] [PDF]