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J. Biol. Chem., Vol. 277, Issue 4, 2732-2739, January 25, 2002
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From the Graduate School of Integrated Science, Yokohama City
University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan
JKTBP proteins are related to a family of
heterogeneous nuclear ribonucleoproteins (hnRNPs) that function in
mRNA biogenesis and mRNA metabolism. JKTBP proteins constituted
of isoforms 1, 2, and 1 The nucleotide sequence reported in this paper has been submitted
to the DDBJ/GenBankTM/EBI Data Bank with
accession number AB066484.
Identification of the Nucleocytoplasmic Shuttling Sequence of
Heterogeneous Nuclear Ribonucleoprotein D-like Protein JKTBP and Its
Interaction with mRNA*
,
6 are localized in the nucleus. We show that
the dominant form JKTBP1 shuttles between the nucleus and the
cytoplasm and interacts with mRNA. Immunofluorescence microscopy
and immunoblotting of the subcellular fractions and overexpression of
JKTBP tagged with green fluorescent protein indicated that
JKTBP1 and JKTBP16, but not JKTBP2, accumulate in the cytoplasm upon
polymerase II transcription inhibition. After release from
inhibition, the return of accumulated cytoplasmic JKTBP to the nucleus
was temperature-dependent. In heterokaryons, green
fluorescent protein-tagged JKTBP1 and JKTBP16 migrated from the HeLa
nucleus to the mouse nucleus, but JKTBP2 did not. Using various JKTBP
deletion mutants, the 25-residue C-terminal tail was identified as a
shuttling sequence like M9. It is conserved in the C-terminal tails of
hnRNP D/AUF1 and type A/B hnRNP/ABBP-1. Analysis of its
sequence-specific interacting protein indicated that JKTBP nuclear
import is mediated by the receptor transportin 1/karyopherin 
2. UV
cross-linking revealed the increased occurrence of JKTBP1 directly
interacting with poly(A)+ RNA in the cytoplasm
following actinomycin D treatment. We discuss a role of JKTBP in
mRNA nuclear export.
*
This work was supported in part by grants-in-aid for
Promotion of Research at Yokohama City University and for Cancer
Research (11) from the Ministry of Health, Labor, and
Welfare.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Inst. of Medical Science, University of Tokyo,
Tokyo 108-0071, Japan.
§
Present address: National Cancer Center Research Inst., Tokyo
104-0045, Japan.
¶
To whom correspondence should be addressed. Tel.:
81-45-787-2214; Fax: 81-45-787-2370; E-mail:
myamada@yokohama-cu.ac.jp.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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