Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M202295200 on July 22, 2002

J. Biol. Chem., Vol. 277, Issue 40, 37260-37271, October 4, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/40/37260    most recent
M202295200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trinel, P.-A.
Right arrow Articles by Poulain, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trinel, P.-A.
Right arrow Articles by Poulain, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Candida albicans Phospholipomannan, a New Member of the Fungal Mannose Inositol Phosphoceramide Family*

Pierre-André TrinelDagger , Emmanuel Maes§, Jean-Pierre Zanetta§, Florence Delplace§, Bernadette Coddeville§, Thierry JouaultDagger , Gérard Strecker§, and Daniel PoulainDagger

From the Dagger  Laboratoire de Mycologie Fondamentale et Appliquée, Inserm EPI 9915, Faculté de Médecine, Pôle Recherche, 59037, Lille Cedex, France, and § Laboratoire de Glycobiologie Structurale et Fonctionnelle, CNRS Unité Mixté de Recherche No. 8576, Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq, France

The pathogenic yeast Candida albicans has the ability to synthesize unique sequences of beta -1,2-oligomannosides that act as adhesins, induce cytokine production, and generate protective antibodies. Depending on the growth conditions, beta -1,2-oligomannosides are associated with different carrier molecules in the cell wall. Structural evidence has been obtained for the presence of these residues in the polysaccharide moiety of the glycolipid, phospholipomannan (PLM). In this study, the refinement of purification techniques led to large quantities of PLM being extracted from Candida albicans cells. A combination of methanolysis, gas chromatography, mass spectrometry, and nuclear magnetic resonance analyses allowed the complete structure of PLM to be deduced. The lipid moiety was shown to consist of a phytoceramide associating a C18/C20 phytosphingosine and C25, C26, or mainly C24 hydroxy fatty acids. The spacer linking the glycan part was identified as a unique structure: -Man-P-Man-Ins-P-. Therefore, in contrast to the major class of membranous glycosphingolipids represented by mannose diinositol phosphoceramide, which is derived from mannose inositol phosphoceramide by the addition of inositol phosphate, PLM seems to be derived from mannose inositol phosphoceramide by the addition of mannose phosphate. In relation to a previous study of the glycan part of the molecule, the assignment of the second phosphorus position leads to the definition of PLM beta -1,2-oligomannosides as unbranched linear structures that may reach up to 19 residues in length. Therefore, PLM appears to be a new type of glycosphingolipid, which is glycosylated extensively through a unique spacer. The conferred hydrophilic properties allow PLM to diffuse into the cell wall in which together with mannan it presents C. albicans beta -1,2-oligomannosides to host cells.

* This work was supported by the "Réseau Infection Fongique" du Ministère Français de l'Education Nationale de la Recherche et de la Technologie (MNERT).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 33-3-20-62-34-20; Fax: 33-3-20-62-34-16; E-mail: dan_poulain@compuserve.com.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J BiochemHome page
E. Maes, C. Mille, X. Trivelli, G. Janbon, D. Poulain, and Y. Guerardel
Molecular Phenotyping of Mannosyltransferases-Deficient Candida albicans Cells by High-Resolution Magic Angle Spinning NMR
J. Biochem., April 1, 2009; 145(4): 413 - 419.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Fradin, M. C. Slomianny, C. Mille, A. Masset, R. Robert, B. Sendid, J. F. Ernst, J. C. Michalski, and D. Poulain
{beta}-1,2 Oligomannose Adhesin Epitopes Are Widely Distributed over the Different Families of Candida albicans Cell Wall Mannoproteins and Are Associated through both N- and O-Glycosylation Processes
Infect. Immun., October 1, 2008; 76(10): 4509 - 4517.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Mille, P. Bobrowicz, P.-A. Trinel, H. Li, E. Maes, Y. Guerardel, C. Fradin, M. Martinez-Esparza, R. C. Davidson, G. Janbon, et al.
Identification of a New Family of Genes Involved in {beta}-1,2-Mannosylation of Glycans in Pichia pastoris and Candida albicans
J. Biol. Chem., April 11, 2008; 283(15): 9724 - 9736.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
C. Simenel, B. Coddeville, M. Delepierre, J.-P. Latge, and T. Fontaine
Glycosylinositolphosphoceramides in Aspergillus Fumigatus
Glycobiology, January 1, 2008; 18(1): 84 - 96.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Sato, X.-l. Yang, T. Yudate, J.-S. Chung, J. Wu, K. Luby-Phelps, R. P. Kimberly, D. Underhill, P. D. Cruz Jr., and K. Ariizumi
Dectin-2 Is a Pattern Recognition Receptor for Fungi That Couples with the Fc Receptor {gamma} Chain to Induce Innate Immune Responses
J. Biol. Chem., December 15, 2006; 281(50): 38854 - 38866.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Jouault, M. El Abed-El Behi, M. Martinez-Esparza, L. Breuilh, P.-A. Trinel, M. Chamaillard, F. Trottein, and D. Poulain
Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling
J. Immunol., October 1, 2006; 177(7): 4679 - 4687.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. J. Heung, C. Luberto, and M. Del Poeta
Role of Sphingolipids in Microbial Pathogenesis
Infect. Immun., January 1, 2006; 74(1): 28 - 39.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Costachel, B. Coddeville, J.-P. Latge, and T. Fontaine
Glycosylphosphatidylinositol-anchored Fungal Polysaccharide in Aspergillus fumigatus
J. Biol. Chem., December 2, 2005; 280(48): 39835 - 39842.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Mille, G. Janbon, F. Delplace, S. Ibata-Ombetta, C. Gaillardin, G. Strecker, T. Jouault, P.-A. Trinel, and D. Poulain
Inactivation of CaMIT1 Inhibits Candida albicans Phospholipomannan {beta}-Mannosylation, Reduces Virulence, and Alters Cell Wall Protein {beta}-Mannosylation
J. Biol. Chem., November 12, 2004; 279(46): 47952 - 47960.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. P. Hobson, C. A. Munro, S. Bates, D. M. MacCallum, J. E. Cutler, S. E. M. Heinsbroek, G. D. Brown, F. C. Odds, and N. A.R. Gow
Loss of Cell Wall Mannosylphosphate in Candida albicans Does Not Influence Macrophage Recognition
J. Biol. Chem., September 17, 2004; 279(38): 39628 - 39635.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
F. Dalle, T. Jouault, P. A. Trinel, J. Esnault, J. M. Mallet, P. d'Athis, D. Poulain, and A. Bonnin
{beta}-1,2- and {alpha}-1,2-Linked Oligomannosides Mediate Adherence of Candida albicans Blastospores to Human Enterocytes In Vitro
Infect. Immun., December 1, 2003; 71(12): 7061 - 7068.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ibata-Ombetta, T. Idziorek, P.-A. Trinel, D. Poulain, and T. Jouault
Candida albicans Phospholipomannan Promotes Survival of Phagocytosed Yeasts through Modulation of Bad Phosphorylation and Macrophage Apoptosis
J. Biol. Chem., April 4, 2003; 278(15): 13086 - 13093.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement