|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 40, 37307-37314, October 4, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Institut für Allgemeine Botanik, Johannes
Gutenberg-Universität, Müllerweg 6, 55099 Mainz, Germany
Light-harvesting complexes (LHC) of higher plants
are composed of at least 10 different proteins. Despite their
pronounced amino acid sequence homology, the LHC of photosystem II show
differences in pigment binding that are interpreted in terms of partly
different functions. By contrast, there is only scarce knowledge about
the pigment composition of LHC of photosystem I, and consequently no
concept of potentially different functions of the various LHCI exists.
For better insight into this issue, we isolated native LHCI-730 and
LHCI-680. Pigment analyses revealed that LHCI-730 binds more
chlorophyll and violaxanthin than LHCI-680. For the first time all LHCI
complexes are now available in their recombinant form; their analysis
allowed further dissection of pigment binding by individual LHCI
proteins and analysis of pigment requirements for LHCI formation. By
these different approaches a correlation between the requirement of a
single chlorophyll species for LHC formation and the chlorophyll
a/b ratio of LHCs could be detected, and
indications regarding occupation of carotenoid-binding sites were
obtained. Additionally the reconstitution approach allowed assignment
of spectral features observed in native LHCI-680 to its components
Lhca2 and Lhca3. It is suggested that excitation energy migrates
from chlorophyll(s) fluorescing at 680 (Lhca3) via those fluorescing at
686/702 nm (Lhca2) or 720 nm (Lhca3) to the photosystem I core chlorophylls.
Pigment Binding of Photosystem I Light-harvesting Proteins*
,
*
This work was supported by Deutsche Forschungsmeinschaft
Grants Schm 1203/2-3 and 2-4.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Institut für
Allgemeine Botanik, Johannes Gutenberg-Universität,
Müllerweg 6, 55099 Mainz, Germany. Tel.: 0049-6131-3924203; Fax:
0049-6131-3923787; E-mail: vschmid@mail.uni-mainz.de
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Wientjes, G. T. Oostergetel, S. Jansson, E. J. Boekema, and R. Croce The Role of Lhca Complexes in the Supramolecular Organization of Higher Plant Photosystem I J. Biol. Chem., March 20, 2009; 284(12): 7803 - 7810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wehner, T. Grasses, and P. Jahns De-epoxidation of Violaxanthin in the Minor Antenna Proteins of Photosystem II, LHCB4, LHCB5, and LHCB6 J. Biol. Chem., August 4, 2006; 281(31): 21924 - 21933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Naumann, E. J. Stauber, A. Busch, F. Sommer, and M. Hippler N-terminal Processing of Lhca3 Is a Key Step in Remodeling of the Photosystem I-Light-harvesting Complex Under Iron Deficiency in Chlamydomonas reinhardtii J. Biol. Chem., May 27, 2005; 280(21): 20431 - 20441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Morosinotto, M. Mozzo, R. Bassi, and R. Croce Pigment-Pigment Interactions in Lhca4 Antenna Complex of Higher Plants Photosystem I J. Biol. Chem., May 27, 2005; 280(21): 20612 - 20619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Storf, S. Jansson, and V. H. R. Schmid Pigment Binding, Fluorescence Properties, and Oligomerization Behavior of Lhca5, a Novel Light-harvesting Protein J. Biol. Chem., February 18, 2005; 280(7): 5163 - 5168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Nield, K. Redding, and M. Hippler Remodeling of Light-Harvesting Protein Complexes in Chlamydomonas in Response to Environmental Changes Eukaryot. Cell, December 1, 2004; 3(6): 1370 - 1380. [Full Text] [PDF]
|
|
|
|
|
|
R. Croce, T. Morosinotto, J. A. Ihalainen, A. Chojnicka, J. Breton, J. P. Dekker, R. van Grondelle, and R. Bassi Origin of the 701-nm Fluorescence Emission of the Lhca2 Subunit of Higher Plant Photosystem I J. Biol. Chem., November 19, 2004; 279(47): 48543 - 48549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wehner, S. Storf, P. Jahns, and V. H. R. Schmid De-epoxidation of Violaxanthin in Light-harvesting Complex I Proteins J. Biol. Chem., June 25, 2004; 279(26): 26823 - 26829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Jensen, A. Haldrup, S. Zhang, and H. V. Scheller The PSI-O Subunit of Plant Photosystem I Is Involved in Balancing the Excitation Pressure between the Two Photosystems J. Biol. Chem., June 4, 2004; 279(23): 24212 - 24217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Kwa, A. Garcia-Martin, A. P. Vegh, B. Strohmann, B. Robert, and P. Braun Hydrogen Bonding in a Model Bacteriochlorophyll-binding Site Drives Assembly of Light Harvesting Complex J. Biol. Chem., April 9, 2004; 279(15): 15067 - 15075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Morosinotto, J. Breton, R. Bassi, and R. Croce The Nature of a Chlorophyll Ligand in Lhca Proteins Determines the Far Red Fluorescence Emission Typical of Photosystem I J. Biol. Chem., December 5, 2003; 278(49): 49223 - 49229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Melkozernov and R. E. Blankenship Structural Modeling of the Lhca4 Subunit of LHCI-730 Peripheral Antenna in Photosystem I Based on Similarity with LHCII J. Biol. Chem., November 7, 2003; 278(45): 44542 - 44551. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |