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J. Biol. Chem., Vol. 277, Issue 40, 37456-37463, October 4, 2002
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From the Regulation of RNA polymerase during initiation,
elongation, and termination of transcription is mediated in part by
interactions with intrinsic regulatory signals encoded in the RNA and
DNA that contact the enzyme. These interactions include contacts to an 8-9-bp RNA:DNA hybrid within the active-site cleft of the enzyme, contacts to the melted nontemplate DNA strand in the vicinity of the
hybrid, contacts to exiting RNA upstream of the hybrid, and contacts to
~20 bp of duplex DNA downstream of the active site. Based on
characterization of an amino acid substitution (G1161R) and a deletion
(
The Downstream DNA Jaw of Bacterial RNA Polymerase Facilitates
Both Transcriptional Initiation and Pausing*
,, and
Department of Microbiology, Stockholm
University, S-10691 Stockholm, Sweden and the § Department
of Bacteriology, University of Wisconsin,
Madison, Wisconsin 53706
1149-1190) in the jaw domain of the bacterial RNA polymerase
largest subunit (
'), we report here that contacts of the jaw domain
to downstream DNA at the leading edge of the transcription complex
contribute to regulation during all three phases of transcription. The
results provide insight into the role of the jaw domain-downstream DNA
contact in transcriptional initiation and pausing and suggest
possible explanations for the previously reported isolation of the jaw
mutants based on reduced ColEI plasmid replication.
*
This work was supported by National Institutes of Health
Grant GM 38660 (to R. L.), grants from the Swedish National
Research Council and the Swedish Research Council (to L. A. I.), and a grant from the Wallenberg Foundation (to J. E.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Fax:
608-262-9865; E-mail: landick@bact.wisc.edu.
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