| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 40, 37512-37518, October 4, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From Rigel, Inc., South San Francisco, California 94080
Inteins are polypeptide sequences found in a
small set of primarily bacterial proteins that promote the splicing of
flanking pre-protein sequences to generate mature protein products.
Inteins can be engineered in a "split and inverted" configuration
such that the protein splicing product is a cyclic polypeptide
consisting of the sequence linking two intein subdomains. We have
engineered a split intein into a retroviral expression system to enable
the intracellular delivery of a library of random cyclic peptides in
human cells. Cyclization of peptides could be detected in cell lysates
using mass spectrometry. A functional genetic screen to identify
5-amino acid-long cyclic peptides that block interleukin-4 mediated IgE class switching in B cells yielded 13 peptides that selectively inhibited germ line
Retrovirally Delivered Random Cyclic Peptide Libraries Yield
Inhibitors of Interleukin-4 Signaling in Human B Cells*
,,
transcription. These results demonstrate the generation of cyclic peptide libraries in human cells
and the power of functional screening to rapidly identify biologically
active peptides.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
These authors contributed equally to this work.
§
Present address: Dept. of Biology, Massachusetts Inst. of
Technology, Cambridge, MA 02139-4307.
¶
To whom correspondence should be addressed: Rigel, Inc., 240 East Grand Ave., South San Francisco, CA 94080. Tel.: 650-624-1102; Fax: 650-624-1133; E-mail: dgpayan@rigel.com.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Hasegawa, A. B. Cowan, N. Nakatsuji, and H. Suemori Efficient Multicistronic Expression of a Transgene in Human Embryonic Stem Cells Stem Cells, July 1, 2007; 25(7): 1707 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Holland, M. J. Powell, C. Franci, E. W. Chan, A. M. Friera, R. E. Atchison, J. McLaughlin, S. E. Swift, E. S. Pali, G. Yam, et al. Multiple Roles for the Receptor Tyrosine Kinase Axl in Tumor Formation Cancer Res., October 15, 2005; 65(20): 9294 - 9303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wolkowicz, G. C. Jager, and G. P. Nolan A Random Peptide Library Fused to CCR5 for Selection of Mimetopes Expressed on the Mammalian Cell Surface via Retroviral Vectors J. Biol. Chem., April 15, 2005; 280(15): 15195 - 15201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Stitz, P. O. Krutzik, and G. P. Nolan Screening of retroviral cDNA libraries for factors involved in protein phosphorylation in signaling cascades Nucleic Acids Res., February 24, 2005; 33(4): e39 - e39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Hale, G. P. Nolan, and R. Wolkowicz Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates Nucleic Acids Res., January 29, 2004; 32(2): e22 - e22. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yang, G. C. Fox Jr., and T. V. Henry-Smith Intein-mediated assembly of a functional beta -glucuronidase in transgenic plants PNAS, March 18, 2003; 100(6): 3513 - 3518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Richards, J. Karpilow, C. Dunn, I. Peterson, A. Maxfield, L. Zharkikh, M. Abedi, A. Hurlburt, J. Hardman, F. Hsu, et al. Genetic Selection for Modulators of a Retinoic-Acid-Responsive Reporter in Human Cells Genetics, March 1, 2003; 163(3): 1047 - 1060. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
