| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 40, 37637-37646, October 4, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,§,
From the Departamento de Bioquímica y
Biología Molecular, Instituto Universitario de
Oncología, Universidad de Oviedo, 33006 Oviedo, Spain
We have identified and cloned a fetal liver
cDNA encoding a new serine proteinase that has been called
matriptase-2. This protein exhibits a domain organization similar to
other members of an emerging family of membrane-bound serine
proteinases known as type II transmembrane serine proteinases.
Matriptase-2 contains a short cytoplasmic domain, a type II
transmembrane sequence, a central region with several modular
structural domains including two CUB (complement
factor C1s/C1r, urchin embryonic growth factor, bone morphogenetic protein) domains and three low density
lipoprotein receptor tandem repeats, and finally, a C-terminal
catalytic domain with all typical features of serine proteinases. The
human matriptase-2 gene maps to 22q12-q13, a location that differs from
all type II transmembrane serine proteinase genes mapped to date.
Immunofluorescence and Western blot analysis of COS-7 cells transfected
with the isolated cDNA confirmed that matriptase-2 is anchored to
the cell surface. Matriptase-2 was expressed in Escherichia
coli, and the purified recombinant protein hydrolyzed synthetic
substrates used for assaying serine proteinases and endogenous proteins
such as type I collagen, fibronectin, and fibrinogen.
Matriptase-2 could also activate single-chain urokinase plasminogen
activator, albeit with low efficiency. These activities were abolished
by inhibitors of serine proteinases but not by inhibitors of other
classes of proteolytic enzymes. Northern blot analysis demonstrated
that matriptase-2 transcripts are only detected at significant levels in both fetal and adult liver, suggesting that this novel serine proteinase may play a specialized role in matrix remodeling processes taking place in this tissue during development or in adult tissues.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ319876.
These authors contributed equally to this manuscript.
§
Recipients of research contracts from Ministerio de Ciencia y
Tecnología, Spain.
¶
To whom correspondence should be addressed: Departamento de
Bioquímica y Biología Molecular, Facultad de Medicina,
Universidad de Oviedo, 33006 Oviedo, Spain. Tel.: 34-985-104201;
Fax: 34-985-103564; E-mail: CLO@correo.uniovi.es.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Du, E. She, T. Gelbart, J. Truksa, P. Lee, Y. Xia, K. Khovananth, S. Mudd, N. Mann, E. M. Y. Moresco, et al. The Serine Protease TMPRSS6 Is Required to Sense Iron Deficiency Science, May 23, 2008; 320(5879): 1088 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Hartikainen, H. Tuhkanen, V. Kataja, M. Eskelinen, M. Uusitupa, V.-M. Kosma, and A. Mannermaa Refinement of the 22q12-q13 Breast Cancer-Associated Region: Evidence of TMPRSS6 as a Candidate Gene in an Eastern Finnish Population Clin. Cancer Res., March 1, 2006; 12(5): 1454 - 1462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. List, R. Szabo, A. Molinolo, V. Sriuranpong, V. Redeye, T. Murdock, B. Burke, B. S. Nielsen, J. S. Gutkind, and T. H. Bugge Deregulated matriptase causes ras-independent multistage carcinogenesis and promotes ras-mediated malignant transformation Genes & Dev., August 15, 2005; 19(16): 1934 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cal, V. Quesada, M. Llamazares, A. Diaz-Perales, C. Garabaya, and C. Lopez-Otin Human Polyserase-2, a Novel Enzyme with Three Tandem Serine Protease Domains in a Single Polypeptide Chain J. Biol. Chem., January 21, 2005; 280(3): 1953 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Hobson, S. Netzel-Arnett, R. Szabo, S. M. Rehault, F. C. Church, D. K. Strickland, D. A. Lawrence, T. M. Antalis, and T. H. Bugge Mouse DESC1 Is Located within a Cluster of Seven DESC1-like Genes and Encodes a Type II Transmembrane Serine Protease That Forms Serpin Inhibitory Complexes J. Biol. Chem., November 5, 2004; 279(45): 46981 - 46994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Quesada, L. M. Sanchez, J. Alvarez, and C. Lopez-Otin Identification and Characterization of Human and Mouse Ovastacin: A NOVEL METALLOPROTEINASE SIMILAR TO HATCHING ENZYMES FROM ARTHROPODS, BIRDS, AMPHIBIANS, AND FISH J. Biol. Chem., June 18, 2004; 279(25): 26627 - 26634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Noel, C Maillard, N Rocks, M Jost, V Chabottaux, N E Sounni, E Maquoi, D Cataldo, and J M Foidart Membrane associated proteases and their inhibitors in tumour angiogenesis J. Clin. Pathol., June 1, 2004; 57(6): 577 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Knappe, F. Wu, M. R. Masikat, J. Morser, and Q. Wu Functional Analysis of the Transmembrane Domain and Activation Cleavage of Human Corin: DESIGN AND CHARACTERIZATION OF A SOLUBLE CORIN J. Biol. Chem., December 26, 2003; 278(52): 52363 - 52370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cal, V. Quesada, C. Garabaya, and C. Lopez-Otin Polyserase-I, a human polyprotease with the ability to generate independent serine protease domains from a single translation product PNAS, August 5, 2003; 100(16): 9185 - 9190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bernocco, B. M. Steiglitz, D. I. Svergun, M. V. Petoukhov, F. Ruggiero, S. Ricard-Blum, C. Ebel, C. Geourjon, G. Deleage, B. Font, et al. Low Resolution Structure Determination Shows Procollagen C-Proteinase Enhancer to be an Elongated Multidomain Glycoprotein J. Biol. Chem., February 21, 2003; 278(9): 7199 - 7205. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
