HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 41, 38693-38699, October 11, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/41/38693    most recent M206075200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brini, M. Articles by Carafoli, E. Search for Related Content PubMed PubMed Citation Articles by Brini, M. Articles by Carafoli, E. Social Bookmarking                      What's this?

Recombinant Expression of the Plasma Membrane Na⁺/Ca²⁺ Exchanger Affects Local and Global Ca²⁺ Homeostasis in Chinese Hamster Ovary Cells^*

Marisa Brini, Sabrina Manni, and Ernesto Carafoli

From the Department of Biochemistry and Center for the Study of Biomembranes of the National Research Council, University of Padova, Viale G. Colombo 3, 35121 Padova and the Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy

The cardiac type Na⁺/Ca²⁺ exchanger (NCX1) has been transiently expressed in Chinese hamster ovary cells, which do not contain an endogenous exchanger, together with aequorin chimeras that are targeted to different intracellular compartments to investigate intracellular Ca²⁺ homeostasis. The expression of NCX decreased the endoplasmic reticulum Ca²⁺ concentration, [Ca²⁺]_er, in resting cells, showing that the exchanger was operative under these conditions. It induced a greater reduction in the height of the mitochondrial and cytosolic Ca²⁺ transients in agonist-stimulated cells than would have been expected from the [Ca²⁺]_er decrease. It also had a major effect on the sub-plasma membrane Ca²⁺ concentration, [Ca²⁺]_pm: after a transient [Ca²⁺]_pm rise induced by the activation of capacitative Ca²⁺ influx, [Ca²⁺]_pm settled to a value about 3-fold higher than in controls. The sustained [Ca²⁺]_pm increase after the transient was due to the operation of the exchanger, either directly by operating in the Ca²⁺ entry mode, or indirectly by removing the Ca²⁺ inhibition on the capacitative Ca²⁺ influx channels.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				E. Aneiros, S. Philipp, A. Lis, M. Freichel, and A. Cavalie Modulation of Ca2+ Signaling by Na+/Ca2+ Exchangers in Mast Cells J. Immunol., January 1, 2005; 174(1): 119 - 130. [Abstract] [Full Text] [PDF]

				C. Rosker, A. Graziani, M. Lukas, P. Eder, M. X. Zhu, C. Romanin, and K. Groschner Ca2+ Signaling by TRPC3 Involves Na+ Entry and Local Coupling to the Na+/Ca2+ Exchanger J. Biol. Chem., April 2, 2004; 279(14): 13696 - 13704. [Abstract] [Full Text] [PDF]