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Originally published In Press as doi:10.1074/jbc.C200410200 on August 29, 2002

J. Biol. Chem., Vol. 277, Issue 42, 39066-39069, October 18, 2002
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ACCELERATED PUBLICATION
Coenzymatic Activity of Randomly Broken or Intact Double-stranded DNAs in Auto and Histone H1 Trans-poly(ADP-ribosylation), Catalyzed by Poly(ADP-ribose) Polymerase (PARP I)*

Ernest KunDagger §, Eva KirstenDagger , and Charles P. OrdahlDagger

From the Department of Anatomy and § Department of Cellular Molecular Pharmacology, San Francisco School of Medicine, University of California, San Francisco, California 94143

The enzymatic transfer of ADP-ribose from NAD to histone H1 (defined as trans-poly(ADP-ribosylation)) or to PARP I (defined as auto-poly(ADP-ribosylation)) was studied with respect to the nature of the DNA required as a coenzyme. Linear double-stranded DNA (dsDNA) containing the MCAT core motif was compared with DNA containing random nicks (discontinuous or dcDNA). The dsDNAs activated trans-poly(ADP-ribosylation) about 5 times more effectively than dcDNA as measured by Vmax. Activation of auto-poly(ADP-ribosylation) by dcDNA was 10 times greater than by dsDNA. The affinity of PARP I toward dcDNA or dsDNA in the auto-poly(ADP-ribosylation) was at least 100-fold lower than in trans-poly(ADP-ribosylation) (Ka = 1400 versus 3-15, respectively). Mg2+ inhibited trans-poly(ADP-ribosylation) and so did dcDNA at concentrations required to maximally activate auto-poly(ADP-ribosylation). Mg2+ activated auto-poly(ADP-ribosylation) of PARP I. These results for the first time demonstrate that physiologically occurring dsDNAs can serve as coenzymes for PARP I and catalyze preferentially trans-poly(ADP- ribosylation), thereby opening the possibility to study the physiologic function of PARP I.

* This research was supported by National Institutes of Health Grants HL-59693 and HL-35561 (to C. P. O.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger All three authors contributed equally to this work.

To whom correspondence should be addressed. Tel.: 415-476-4051; Fax: 415-476-4845; E-mail: ordahl@itsa.ucsf.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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