JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M206400200 on August 2, 2002

J. Biol. Chem., Vol. 277, Issue 42, 39209-39216, October 18, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/42/39209    most recent
M206400200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guaita, S.
Right arrow Articles by Baulida, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guaita, S.
Right arrow Articles by Baulida, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Snail Induction of Epithelial to Mesenchymal Transition in Tumor Cells Is Accompanied by MUC1 Repression and ZEB1 Expression*

Sandra GuaitaDagger §, Isabel PuigDagger §||, Clara FrancíDagger , Marta GarridoDagger , David DomínguezDagger , Eduard BatlleDagger **, Elena SanchoDagger **, Shoukat DedharDagger Dagger , Antonio García de HerrerosDagger §§, and Josep BaulidaDagger ¶¶

From the Dagger  Unitat de Biologia Cellular i Molecular, Institut Municipal d'Investigació Mèdica, Universitat Pompeu Fabra, E-08003 Barcelona, Spain and the Dagger Dagger  Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6H 3Z6, Canada

E-cadherin protein plays a key role in the establishment and maintenance of adherent junctions. Recent evidence implicates the transcription factor Snail in the blockage of E-cadherin expression in fibroblasts and some epithelial tumor cells through direct binding to three E-boxes in the E-cadherin promoter. Transfection of Snail into epithelial cells leads to a more fibroblastic phenotype. Cells expressing Snail presented a scattered flattened phenotype with low intercellular contacts. Other epithelial markers like Cytokeratin 18 or MUC1 were also repressed. The effects of Snail on MUC1 transcription were mediated by two E-boxes present in the proximal promoter. Snail also induced expression of the mesenchymal markers fibronectin and LEF1 and the transcription repressor ZEB1. ZEB1 and Snail had a similar pattern of expression in epithelial cell lines, and both were induced by overexpression of ILK1, a kinase that causes the loss of E-cadherin and the acquisition of a fibroblastic phenotype. Snail overexpression in several cell lines raised ZEB1 RNA levels and increased the activity of ZEB1 promoter. ZEB1 could also repress E-cadherin and MUC1 promoters but less strongly than Snail. However, since ZEB1 expression persisted after Snail was down-regulated, ZEB1 may regulate epithelial genes in several tumor cell lines.

* This work was supported by Ministerio de Ciencia y Tecnología Grant PM99-0132 (to A. G. H.) and Fondo de Investigaciones Sanitarias Grant 01/3060 (to J. B.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors made equivalent contributions to this work.

Recipient of a predoctoral fellowship from Ministerio de Ciencia y Tecnología (Formación de Personal Investigador) and supported by a predoctoral fellowship "Xavier Lamas" awarded by Institut Municipal d'Assistència Sanitària for the initial work.

** Current address: Dept. of Immunology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584 CX, The Netherlands.

|| Recipient of a predoctoral fellowship from Ministerio de Educación (Formacion del Profesorado Universitario).

§§ To whom correspondence may be addressed: Institut Municipal d'Investigació Mèdica, c/Doctor Aiguader, 80, E-08003 Barcelona, Spain. Tel.: 34-93-221-1009; Fax: 34-93-221-3237; E-mail: agarcia@imim.es.

¶¶ To whom correspondence may be addressed: Institut Municipal d'Investigació Mèdica, c/Doctor Aiguader, 80, E-08003 Barcelona, Spain. Tel.: 34-93-221-1009; Fax: 34-93-221-3237; E-mail: jbaulida@imim.es.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
N. Herranz, D. Pasini, V. M. Diaz, C. Franci, A. Gutierrez, N. Dave, M. Escriva, I. Hernandez-Munoz, L. Di Croce, K. Helin, et al.
Polycomb Complex 2 Is Required for E-cadherin Repression by the Snail1 Transcription Factor
Mol. Cell. Biol., August 1, 2008; 28(15): 4772 - 4781.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. Solanas, M. Porta-de-la-Riva, C. Agusti, D. Casagolda, F. Sanchez-Aguilera, M. J. Larriba, F. Pons, S. Peiro, M. Escriva, A. Munoz, et al.
E-cadherin controls {beta}-catenin and NF-{kappa}B transcriptional activity in mesenchymal gene expression
J. Cell Sci., July 1, 2008; 121(13): 2224 - 2234.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. R. Graham, H. E. Zhau, V. A. Odero-Marah, A. O. Osunkoya, K. S. Kimbro, M. Tighiouart, T. Liu, J. W. Simons, and R. M. O'Regan
Insulin-like Growth Factor-I-Dependent Up-regulation of ZEB1 Drives Epithelial-to-Mesenchymal Transition in Human Prostate Cancer Cells
Cancer Res., April 1, 2008; 68(7): 2479 - 2488.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Beltran, I. Puig, C. Pena, J. M. Garcia, A. B. Alvarez, R. Pena, F. Bonilla, and A. G. de Herreros
A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition
Genes & Dev., March 15, 2008; 22(6): 756 - 769.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Liu, S. El-Naggar, D. S. Darling, Y. Higashi, and D. C. Dean
Zeb1 links epithelial-mesenchymal transition and cellular senescence
Development, February 1, 2008; 135(3): 579 - 588.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Olmeda, G. Moreno-Bueno, J. M. Flores, A. Fabra, F. Portillo, and A. Cano
SNAI1 Is Required for Tumor Growth and Lymph Node Metastasis of Human Breast Carcinoma MDA-MB-231 Cells
Cancer Res., December 15, 2007; 67(24): 11721 - 11731.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Konopka, J. Tekiela, M. Iverson, C. Wells, and S. A. Duncan
Junctional Adhesion Molecule-A Is Critical for the Formation of Pseudocanaliculi and Modulates E-cadherin Expression in Hepatic Cells
J. Biol. Chem., September 21, 2007; 282(38): 28137 - 28148.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
F. Wang, C. Sloss, X. Zhang, S. W. Lee, and J. C. Cusack
Membrane-Bound Heparin-Binding Epidermal Growth Factor Like Growth Factor Regulates E-Cadherin Expression in Pancreatic Carcinoma Cells
Cancer Res., September 15, 2007; 67(18): 8486 - 8493.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Wang, M. Zheng, G. Liu, W. Xia, P. J. McKeown-Longo, M.-C. Hung, and J. Zhao
Kruppel-Like Factor 8 Induces Epithelial to Mesenchymal Transition and Epithelial Cell Invasion
Cancer Res., August 1, 2007; 67(15): 7184 - 7193.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. B. Palmer, P. Majumder, M. R. Green, P. A. Wade, and J. M. Boss
A 3' Enhancer Controls Snail Expression in Melanoma Cells
Cancer Res., July 1, 2007; 67(13): 6113 - 6120.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
E. Buck, A. Eyzaguirre, S. Barr, S. Thompson, R. Sennello, D. Young, K. K. Iwata, N. W. Gibson, P. Cagnoni, and J. D. Haley
Loss of homotypic cell adhesion by epithelial-mesenchymal transition or mutation limits sensitivity to epidermal growth factor receptor inhibition
Mol. Cancer Ther., February 1, 2007; 6(2): 532 - 541.
[Abstract] [Full Text] [PDF]

Home page
 ASH Education BookHome page
J. P. Radich
The Biology of CML Blast Crisis
Hematology, January 1, 2007; 2007(1): 384 - 391.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Takkunen, R. Grenman, M. Hukkanen, M. Korhonen, A. Garcia de Herreros, and I. Virtanen
Snail-dependent and -independent Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Cells
J. Histochem. Cytochem., November 1, 2006; 54(11): 1263 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. R.E. Rees, B. A. Onwuegbusi, V. E. Save, D. Alderson, and R. C. Fitzgerald
In vivo and In vitro Evidence for Transforming Growth Factor-{beta}1-Mediated Epithelial to Mesenchymal Transition in Esophageal Adenocarcinoma
Cancer Res., October 1, 2006; 66(19): 9583 - 9590.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. J. Christiansen and A. K. Rajasekaran
Reassessing Epithelial to Mesenchymal Transition as a Prerequisite for Carcinoma Invasion and Metastasis.
Cancer Res., September 1, 2006; 66(17): 8319 - 8326.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Saito, M. Nagai, and M. Ladanyi
SYT-SSX1 and SYT-SSX2 Interfere with Repression of E-Cadherin by Snail and Slug: A Potential Mechanism for Aberrant Mesenchymal to Epithelial Transition in Human Synovial Sarcoma.
Cancer Res., July 15, 2006; 66(14): 6919 - 6927.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Dohadwala, S.-C. Yang, J. Luo, S. Sharma, R. K. Batra, M. Huang, Y. Lin, L. Goodglick, K. Krysan, M. C. Fishbein, et al.
Cyclooxygenase-2-Dependent Regulation of E-Cadherin: Prostaglandin E2 Induces Transcriptional Repressors ZEB1 and Snail in Non-Small Cell Lung Cancer.
Cancer Res., May 15, 2006; 66(10): 5338 - 5345.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. S. Spoelstra, N. G. Manning, Y. Higashi, D. Darling, M. Singh, K. R. Shroyer, R. R. Broaddus, K. B. Horwitz, and J. K. Richer
The Transcription Factor ZEB1 Is Aberrantly Expressed in Aggressive Uterine Cancers.
Cancer Res., April 1, 2006; 66(7): 3893 - 3902.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. P. Radich, H. Dai, M. Mao, V. Oehler, J. Schelter, B. Druker, C. Sawyers, N. Shah, W. Stock, C. L. Willman, et al.
Gene expression changes associated with progression and response in chronic myeloid leukemia
PNAS, February 21, 2006; 103(8): 2794 - 2799.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. L. Yauch, T. Januario, D. A. Eberhard, G. Cavet, W. Zhu, L. Fu, T. Q. Pham, R. Soriano, J. Stinson, S. Seshagiri, et al.
Epithelial versus Mesenchymal Phenotype Determines In vitro Sensitivity and Predicts Clinical Activity of Erlotinib in Lung Cancer Patients
Clin. Cancer Res., December 15, 2005; 11(24): 8686 - 8698.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. Pena, J. M. Garcia, J. Silva, V. Garcia, R. Rodriguez, I. Alonso, I. Millan, C. Salas, A. G. de Herreros, A. Munoz, et al.
E-cadherin and vitamin D receptor regulation by SNAIL and ZEB1 in colon cancer: clinicopathological correlations
Hum. Mol. Genet., November 15, 2005; 14(22): 3361 - 3370.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J.-Y. Shih, M.-F. Tsai, T.-H. Chang, Y.-L. Chang, A. Yuan, C.-J. Yu, S.-B. Lin, G.-Y. Liou, M.-L. Lee, J. J.W. Chen, et al.
Transcription Repressor Slug Promotes Carcinoma Invasion and Predicts Outcome of Patients with Lung Adenocarcinoma
Clin. Cancer Res., November 15, 2005; 11(22): 8070 - 8078.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Thomson, E. Buck, F. Petti, G. Griffin, E. Brown, N. Ramnarine, K. K. Iwata, N. Gibson, and J. D. Haley
Epithelial to Mesenchymal Transition Is a Determinant of Sensitivity of Non-Small-Cell Lung Carcinoma Cell Lines and Xenografts to Epidermal Growth Factor Receptor Inhibition
Cancer Res., October 15, 2005; 65(20): 9455 - 9462.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Jorda, D. Olmeda, A. Vinyals, E. Valero, E. Cubillo, A. Llorens, A. Cano, and A. Fabra
Upregulation of MMP-9 in MDCK epithelial cell line in response to expression of the Snail transcription factor
J. Cell Sci., August 1, 2005; 118(15): 3371 - 3385.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
B. De Craene, B. Gilbert, C. Stove, E. Bruyneel, F. van Roy, and G. Berx
The Transcription Factor Snail Induces Tumor Cell Invasion through Modulation of the Epithelial Cell Differentiation Program
Cancer Res., July 15, 2005; 65(14): 6237 - 6244.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Barrallo-Gimeno and M. A. Nieto
The Snail genes as inducers of cell movement and survival: implications in development and cancer
Development, July 15, 2005; 132(14): 3151 - 3161.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
H. Yamasaki, T. Sekimoto, T. Ohkubo, T. Douchi, Y. Nagata, M. Ozawa, and Y. Yoneda
Zinc finger domain of Snail functions as a nuclear localization signal for importin {beta}-mediated nuclear import pathway
Genes Cells, May 1, 2005; 10(5): 455 - 464.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. I. Yook, X.-Y. Li, I. Ota, E. R. Fearon, and S. J. Weiss
Wnt-dependent Regulation of the E-cadherin Repressor Snail
J. Biol. Chem., March 25, 2005; 280(12): 11740 - 11748.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
Y. Uchikado, S. Natsugoe, H. Okumura, T. Setoyama, M. Matsumoto, S. Ishigami, and T. Aikou
Slug Expression in the E-cadherin Preserved Tumors Is Related to Prognosis in Patients with Esophageal Squamous Cell Carcinoma
Clin. Cancer Res., February 1, 2005; 11(3): 1174 - 1180.
[Abstract] [Full Text] [PDF]

Home page
 Crit. Rev. Oral Biol. Med.Home page
S.S. Prime, M. Davies, M. Pring, and I.C. Paterson
THE ROLE OF TGF-{beta} IN EPITHELIAL MALIGNANCY AND ITS RELEVANCE TO THE PATHOGENESIS OF ORAL CANCER (PART II)
Crit. Rev. Oral. Biol. Med., November 1, 2004; 15(6): 337 - 347.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. L. Huff, L. M. Hansen, and J. V. Solnick
Gastric Transcription Profile of Helicobacter pylori Infection in the Rhesus Macaque
Infect. Immun., September 1, 2004; 72(9): 5216 - 5226.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Vega, A. V. Morales, O. H. Ocana, F. Valdes, I. Fabregat, and M. A. Nieto
Snail blocks the cell cycle and confers resistance to cell death
Genes & Dev., May 15, 2004; 18(10): 1131 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Prindull and D. Zipori
Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm
Blood, April 15, 2004; 103(8): 2892 - 2899.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Peinado, M. Quintanilla, and A. Cano
Transforming Growth Factor {beta}-1 Induces Snail Transcription Factor in Epithelial Cell Lines: MECHANISMS FOR EPITHELIAL MESENCHYMAL TRANSITIONS
J. Biol. Chem., May 30, 2003; 278(23): 21113 - 21123.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.