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J. Biol. Chem., Vol. 277, Issue 43, 40368-40374, October 25, 2002
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From the Laboratory of Developmental Physiology and Molecular
Biology, Katholieke Universiteit Leuven, Naamsestraat 59, B-3000 Leuven, Belgium
Neuropeptides regulate most, if not all,
biological processes in the animal kingdom, but only seven have been
isolated and sequenced from Drosophila melanogaster. In
analogy with the proteomics technology, where all proteins expressed in
a cell or tissue are analyzed, the peptidomics approach aims at the
simultaneous identification of the whole peptidome of a cell or tissue,
i.e. all expressed peptides with their posttranslational
modifications. Using nanoscale liquid chromatography combined with
tandem mass spectrometry and data base mining, we analyzed the
peptidome of the larval Drosophila central nervous system
at the amino acid sequence level. We were able to provide biochemical
evidence for the presence of 28 neuropeptides using an extract of only
50 larval Drosophila central nervous systems. Eighteen of
these peptides are encoded in previously cloned or annotated precursor
genes, although not all of them were predicted correctly. Eleven of
these peptides were never purified before. Eight other peptides are
entirely novel and are encoded in five different, not yet annotated
genes. This neuropeptide expression profiling study also opens
perspectives for other eukaryotic model systems, for which genome
projects are completed or in progress.
Peptidomics of the Larval Drosophila melanogaster
Central Nervous System*
*
This project was sponsored by the Flemish Science Foundation
(FWO) Grants G.0356.98, G.0187.00, and G.0175.02 and by the
Katholieke Universiteit Leuven Grant GOA/2000/04.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 32-1632-4260;
Fax: 32-1632-3912; E-mail: liliane.schoofs@bio.kuleuven.ac.be.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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