Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M109443200 on August 22, 2002

J. Biol. Chem., Vol. 277, Issue 43, 41046-41059, October 25, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/43/41046    most recent
M109443200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Slevin, M.
Right arrow Articles by Gaffney, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slevin, M.
Right arrow Articles by Gaffney, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Angiogenic Oligosaccharides of Hyaluronan Induce Multiple Signaling Pathways Affecting Vascular Endothelial Cell Mitogenic and Wound Healing Responses*

Mark SlevinDagger §, Shant Kumar, and John GaffneyDagger

From the Dagger  Department of Biological Sciences, Manchester Metropolitan University, Manchester M1 5GD, United Kingdom and the  Department of Pathological Sciences, Stopford Building, Manchester Victoria University, Manchester M1 5GD, United Kingdom

Hyaluronan (HA) is a large nonsulfated glycosaminoglycan and an important regulator of angiogenesis, in particular, the growth and migration of vascular endothelial cells. We have identified some of the key intermediates responsible for induction of mitogenesis and wound recovery. Treatment of bovine aortic endothelial cells with oligosaccharides of hyaluronan (o-HA) resulted in rapid tyrosine phosphorylation and plasma membrane translocation of phospholipase Cgamma 1 (PLCgamma 1). Cytoplasmic loading with inhibitory antibodies to PLCgamma 1, Gbeta , and Galpha i/o/t/z inhibited activation of extracellular-regulated kinase 1/2 (ERK1/2). Treatment with the Galpha i/o inhibitor, pertussis toxin, reduced o-HA-induced PLCgamma 1 tyrosine phosphorylation, protein kinase C (PKC) alpha  and beta 1/2 membrane translocation, ERK1/2 activation, mitogenesis, and wound recovery, suggesting a mechanism for o-HA-induced angiogenesis through G-proteins, PLCgamma 1, and PKC. In particular, we demonstrated a possible role for PKCalpha in mitogenesis and PKCbeta 1/2 in wound recovery. Using antisense oligonucleotides and the Ras farnesylation inhibitor FTI-277, we showed that o-HA-induced bovine aortic endothelial cell proliferation, wound recovery, and ERK1/2 activation were also partially dependent on Ras activation, and that o-HA-stimulated tyrosine phosphorylation of the adapter protein Shc, as well as its association with Sos1. Binding of Src to Shc was required for its activation and for Ras-dependent activation of ERK1/2, cell proliferation, and wound recovery. Neither Src nor Ras activation was inhibited by pertussis toxin, suggesting that their activation was independent of heterotrimeric G-proteins. However, the specific Src kinase inhibitor PP2 inhibited Gbeta subunit co-precipitation with PLCgamma 1, suggesting a possible role for Src in activation of PLCgamma 1 and interaction between two distinct o-HA-induced signaling pathways.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Fax: 0161247-6365; E-mail: m.a.slevin@mmu.ac.uk.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Yamasaki, J. Muto, K. R. Taylor, A. L. Cogen, D. Audish, J. Bertin, E. P. Grant, A. J. Coyle, A. Misaghi, H. M. Hoffman, et al.
NLRP3/Cryopyrin Is Necessary for Interleukin-1{beta} (IL-1{beta}) Release in Response to Hyaluronan, an Endogenous Trigger of Inflammation in Response to Injury
J. Biol. Chem., May 8, 2009; 284(19): 12762 - 12771.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Vigetti, M. Viola, E. Karousou, M. Rizzi, P. Moretto, A. Genasetti, M. Clerici, V. C. Hascall, G. De Luca, and A. Passi
Hyaluronan-CD44-ERK1/2 Regulate Human Aortic Smooth Muscle Cell Motility during Aging
J. Biol. Chem., February 15, 2008; 283(7): 4448 - 4458.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Singleton, R. Salgia, L. Moreno-Vinasco, J. Moitra, S. Sammani, T. Mirzapoiazova, and J. G. N. Garcia
CD44 Regulates Hepatocyte Growth Factor-mediated Vascular Integrity: ROLE OF c-Met, Tiam1/Rac1, DYNAMIN 2, AND CORTACTIN
J. Biol. Chem., October 19, 2007; 282(42): 30643 - 30657.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Gerecht, J. A. Burdick, L. S. Ferreira, S. A. Townsend, R. Langer, and G. Vunjak-Novakovic
Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells
PNAS, July 3, 2007; 104(27): 11298 - 11303.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Singleton, S. M. Dudek, S.-F. Ma, and J. G. N. Garcia
Transactivation of Sphingosine 1-Phosphate Receptors Is Essential for Vascular Barrier Regulation: NOVEL ROLE FOR HYALURONAN AND CD44 RECEPTOR FAMILY
J. Biol. Chem., November 10, 2006; 281(45): 34381 - 34393.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
A. Al'Qteishat, J. Gaffney, J. Krupinski, F. Rubio, D. West, S. Kumar, P. Kumar, N. Mitsios, and M. Slevin
Changes in hyaluronan production and metabolism following ischaemic stroke in man
Brain, August 1, 2006; 129(8): 2158 - 2176.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. Cao, R. C. Savani, M. Fehrenbach, C. Lyons, L. Zhang, G. Coukos, and H. M. DeLisser
Involvement of Endothelial CD44 during in Vivo Angiogenesis
Am. J. Pathol., July 1, 2006; 169(1): 325 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. Shi, Z.-H. Duan, R. Papay, E. Pluskota, R. J. Gaivin, C. A. de la Motte, E. F. Plow, and D. M. Perez
Novel {alpha}1-Adrenergic Receptor Signaling Pathways: Secreted Factors and Interactions with the Extracellular Matrix
Mol. Pharmacol., July 1, 2006; 70(1): 129 - 142.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Milan, C. Charalambous, R. Elhag, T. C. Chen, W. Li, S. Guan, F. M. Hofman, and R. Zidovetzki
Multiple signaling pathways are involved in endothelin-1-induced brain endothelial cell migration
Am J Physiol Cell Physiol, July 1, 2006; 291(1): C155 - C164.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kumari, B. A. Baggenstoss, A. L. Parker, and P. H. Weigel
Mutation of Two Intramembrane Polar Residues Conserved within the Hyaluronan Synthase Family Alters Hyaluronan Product Size
J. Biol. Chem., April 28, 2006; 281(17): 11755 - 11760.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
M. Zareie, A. S. De Vriese, L. H. P. Hekking, P. M. ter Wee, C. G. Schalkwijk, B. A. J. Driesprong, I. L. Schadee-Eestermans, R. H. J. Beelen, N. Lameire, and J. van den Born
Immunopathological changes in a uraemic rat model for peritoneal dialysis
Nephrol. Dial. Transplant., July 1, 2005; 20(7): 1350 - 1361.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Takahashi, L. Li, M. Kamiryo, T. Asteriou, A. Moustakas, H. Yamashita, and P. Heldin
Hyaluronan Fragments Induce Endothelial Cell Differentiation in a CD44- and CXCL1/GRO1-dependent Manner
J. Biol. Chem., June 24, 2005; 280(25): 24195 - 24204.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
V. B. Lokeshwar, W. H. Cerwinka, and B. L. Lokeshwar
HYAL1 Hyaluronidase: A Molecular Determinant of Bladder Tumor Growth and Invasion
Cancer Res., March 15, 2005; 65(6): 2243 - 2250.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
N. T. Seyfried, C. D. Blundell, A. J. Day, and A. Almond
Preparation and application of biologically active fluorescent hyaluronan oligosaccharides
Glycobiology, March 1, 2005; 15(3): 303 - 312.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. R. Taylor, J. M. Trowbridge, J. A. Rudisill, C. C. Termeer, J. C. Simon, and R. L. Gallo
Hyaluronan Fragments Stimulate Endothelial Recognition of Injury through TLR4
J. Biol. Chem., April 23, 2004; 279(17): 17079 - 17084.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. F. Thorne, J. W. Legg, and C. M. Isacke
The role of the CD44 transmembrane and cytoplasmic domains in co-ordinating adhesive and signalling events
J. Cell Sci., January 22, 2004; 117(3): 373 - 380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. L. DeAngelis, L. C. Oatman, and D. F. Gay
Rapid Chemoenzymatic Synthesis of Monodisperse Hyaluronan Oligosaccharides with Immobilized Enzyme Reactors
J. Biol. Chem., September 12, 2003; 278(37): 35199 - 35203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. N. Sugahara, T. Murai, H. Nishinakamura, H. Kawashima, H. Saya, and M. Miyasaka
Hyaluronan Oligosaccharides Induce CD44 Cleavage and Promote Cell Migration in CD44-expressing Tumor Cells
J. Biol. Chem., August 22, 2003; 278(34): 32259 - 32265.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement