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J. Biol. Chem., Vol. 277, Issue 43, 41183-41191, October 25, 2002
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From the Department of Molecular and Cell Biology,
University of California, Berkeley, California 94720-3206
Human damaged DNA-binding protein (DDB) is a
heterodimer of p48/DDB2 and p127/DDB1 subunits. Mutations in DDB2 are
responsible for Xeroderma Pigmentosum group E, but no mutants of
mammalian DDB1 have been described. To study DDB1, the
Schizosaccharomyces pombe DDB1 sequence homologue
(ddb1+) was cloned, and a ddb1
deletion strain was constructed. The gene is not essential; however,
mutant cells showed a 37% impairment in colony-forming ability, an
elongated phenotype, and abnormal nuclei. The ddb1
Characterization of a Schizosaccharomyces pombe
Strain Deleted for a Sequence Homologue of the Human Damaged DNA
Binding 1 (DDB1) Gene*
,
strain was sensitive to UV irradiation, X-rays, methylmethane
sulfonate, and thiabendazole, and these sensitivities were compared
with those of the well characterized rad13,
rhp51, and cds1 mutant strains. Ddb1p
showed nuclear and nucleolar localization, and the aberrant nuclear
structures observed in the ddb1 strain suggest a role
for Ddb1p in chromosome segregation.
*
This work was supported by National Institutes of Health
Grants 1RO1GM59424 and P30ES08196.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported in part by the H. Wheeler Fellowship Fund.
§
Present address: Lawrence Berkeley National Laboratory, MS 74-157, 1 Cyclotron Rd., Berkeley, CA 94720.
¶
To whom correspondence should be addressed: Dept. of Molecular
and Cell Biology, 229 Stanley Hall, University of California, Berkeley,
CA, 94720-3206. Tel.: 510-642-7583; Fax: 510-643-9290; E-mail:
slinn@socrates.berkeley.edu.
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