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J. Biol. Chem., Vol. 277, Issue 44, 41736-41743, November 1, 2002
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From the § CNRS/ENSL 5665, Ecole Normale
Supérieure de Lyon, 46 Allée d'Italie, F-69364 Lyon,
France and the ¶ Department of Biochemistry and Molecular
Genetics, University of Virginia, Charlottesville, Virginia 22908
Insulators are sequences that uncouple adjacent
chromosome domains. Here we have shown that Saccharomyces
cerevisiae Rap1p and Abf1p proteins are endowed with a potent
insulating capacity. Insulating domains in Rap1p coincide with
previously described transcription activation domains, whereas four
adjacent subdomains spanning the whole of the Abf1p C terminus
(440-731) were found to display autonomous insulating capacity. That
both Rap1p and Abf1p silencing domains either contain or largely
overlap with an insulating domain suggests that insulation conveys some
undefined chromosome organization capacity that also contributes a
function in silencing. Together with Reb1p and Tbf1p, previously
involved in the activity of Saccharomyces cerevisiae
subtelomeric insulators, insulating potential emerges as a
supplementary common property of General Regulatory Factors (GRFs).
Thus GRFs, which bind to sites scattered throughout the genome
within promoters, would not only play a key role in regulating gene
expression but also partition the genome in functionally independent domains.
General Regulatory Factors (GRFs) as Genome Partitioners*
§,
*
This work was supported by La Ligue Nationale contre le
Cancer (to E. G.) and the National Institutes of Health (to R. L.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 33-472728162;
Fax: 33-472728686; E-mail: Genevieve.Fourel@ens-lyon.fr.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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