JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M205504200 on August 26, 2002

J. Biol. Chem., Vol. 277, Issue 44, 42088-42096, November 1, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/44/42088    most recent
M205504200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gong, D.
Right arrow Articles by Zhu, J.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gong, D.
Right arrow Articles by Zhu, J.-K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Constitutive Activation and Transgenic Evaluation of the Function of an Arabidopsis PKS Protein Kinase*

Deming Gong, Changqing Zhang, Xiuyin Chen, Zhizhong Gong, and Jian-Kang ZhuDagger

From the Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

A novel family of SOS2 (salt overly sensitive 2)-like protein kinase genes (designated PKSes) have been recently identified in Arabidopsis. The biochemical characteristics as well as in vivo roles of most of the PKSes are unclear at present. In this work, we isolated and characterized one of the PKSes, PKS18. PKS18 was expressed in leaves of mature Arabidopsis plants. The glutathione S-transferase (GST)-PKS18 fusion protein was inactive by itself in substrate phosphorylation. An activation loop Thr169 to Asp mutation, however, highly activated this kinase in vitro (designated PKS18T/D). Kinase activity of the PKS18T/D preferred Mn2+ to Mg2+. The activated kinase showed a substrate specificity, and high catalytic efficiency for a peptide substrate p3 and for ATP. Interestingly, PKS18T/D transgenic plants were hypersensitive to the phytohormone abscisic acid (ABA) in seed germination and seedling growth, whereas silencing the kinase gene by RNA interference (RNAi) conferred ABA-insensitivity, indicating the involvement of PKS18 in plant ABA signaling.

* This work was supported by National Institutes of Health Grant R01GM59138 (to J.-K. Z.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Plant Sciences, University of Arizona, Tucson, Arizona 85721. Tel.: 520-626-2229; Fax: 520-621-7186; E-mail: jkzhu@ag.arizona.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
G. K. Pandey, J. J. Grant, Y. H. Cheong, B.-G. Kim, L. G. Li, and S. Luan
Calcineurin-B-Like Protein CBL9 Interacts with Target Kinase CIPK3 in the Regulation of ABA Response in Seed Germination
Mol Plant, March 1, 2008; 1(2): 238 - 248.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Burza, I. Pekala, J. Sikora, P. Siedlecki, P. Malagocki, M. Bucholc, L. Koper, P. Zielenkiewicz, M. Dadlez, and G. Dobrowolska
Nicotiana tabacum Osmotic Stress-activated Kinase Is Regulated by Phosphorylation on Ser-154 and Ser-158 in the Kinase Activation Loop
J. Biol. Chem., November 10, 2006; 281(45): 34299 - 34311.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Kelner, I. Pekala, S. Kaczanowski, G. Muszynska, D. G. Hardie, and G. Dobrowolska
Biochemical Characterization of the Tobacco 42-kD Protein Kinase Activated by Osmotic Stress
Plant Physiology, October 1, 2004; 136(2): 3255 - 3265.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. K. Pandey, Y. H. Cheong, K.-N. Kim, J. J. Grant, L. Li, W. Hung, C. D'Angelo, S. Weinl, J. Kudla, and S. Luan
The Calcium Sensor Calcineurin B-Like 9 Modulates Abscisic Acid Sensitivity and Biosynthesis in Arabidopsis
PLANT CELL, July 1, 2004; 16(7): 1912 - 1924.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Gong, Y. Guo, K. S. Schumaker, and J.-K. Zhu
The SOS3 Family of Calcium Sensors and SOS2 Family of Protein Kinases in Arabidopsis
Plant Physiology, March 1, 2004; 134(3): 919 - 926.
[Full Text] [PDF]

Home page
 J Exp BotHome page
M. Seki, M. Satou, T. Sakurai, K. Akiyama, K. Iida, J. Ishida, M. Nakajima, A. Enju, M. Narusaka, M. Fujita, et al.
RIKEN Arabidopsis full-length (RAFL) cDNA and its applications for expression profiling under abiotic stress conditions
J. Exp. Bot., January 2, 2004; 55(395): 213 - 223.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Ohta, Y. Guo, U. Halfter, and J.-K. Zhu
A novel domain in the protein kinase SOS2 mediates interaction with the protein phosphatase 2C ABI2
PNAS, September 30, 2003; 100(20): 11771 - 11776.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Suzuki, M. G. Ketterling, Q.-B. Li, and D. R. McCarty
Viviparous1 Alters Global Gene Expression Patterns through Regulation of Abscisic Acid Signaling
Plant Physiology, July 1, 2003; 132(3): 1664 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Hrabak, C. W.M. Chan, M. Gribskov, J. F. Harper, J. H. Choi, N. Halford, J. Kudla, S. Luan, H. G. Nimmo, M. R. Sussman, et al.
The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases
Plant Physiology, June 1, 2003; 132(2): 666 - 680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.