|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 45, 42603-42612, November 8, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the We have shown previously that the
muscarinic agonist, carbachol (CCh), transactivates the epidermal
growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase
activation. EGFr phosphorylation causes extracellular signal-regulated
kinase (ERK) activation and inhibits CCh-stimulated chloride secretion
across intestinal epithelial cells. Here we investigated whether
CCh-stimulated EGFr transactivation involves EGFr ligand release.
Pre-incubation of T84 cell monolayers with a
neutralizing antibody to the EGFr ligand binding domain decreased
CCh-induced phosphorylation of EGFr and ERK. CCh-stimulated efflux of
86Rb+ from T84 cell monolayers,
which parallels changes in chloride secretion, was potentiated by
anti-EGFr pre-incubation. Anti-EGFr did not reduce CCh-stimulated Pyk-2
phosphorylation. Co-incubation with the Src kinase inhibitor PP2 and
anti-EGFr had an additive inhibitory effect on CCh-induced ERK
phosphorylation greater than either inhibitor alone. CCh caused the
basolateral release of transforming growth factor
Transactivation of the Epidermal Growth Factor Receptor in
Colonic Epithelial Cells by Carbachol Requires Extracellular Release of
Transforming Growth Factor-
*
,,¶
Department of Medicine, University of
California, School of Medicine, San Diego, California 92103 and
§ Vanderbilt University Medical Center and Veterans Affairs
Medical Center, Nashville, Tennessee 37232
(TGF-)
into T84 cell bathing media. A metalloproteinase
inhibitor, WAY171318, reduced CCh-induced phosphorylation of ERK and
completely blocked EGFr phosphorylation and TGF- release. We
conclude that CCh-stimulated EGFr transactivation and subsequent ERK
activation, a pathway that limits CCh-induced chloride secretion, is
mediated by metalloproteinase-dependent extracellular
release of TGF- and intracellular Src activation. These findings
have important implications for our understanding of the role of growth
factors in regulating epithelial ion secretion.
*
This work was supported in part by a Research Fellowship
award (to D. F. M.) and Career Development and First awards (to
S. J. K.) from the Crohn's and Colitis Foundation of America and by
National Institutes of Health Grants CA46413 (to R. J. C.) and
DK28305 (to K. E. B.). A preliminary account of a portion of these
studies was presented at the 2001 annual meeting of the American
Gastroenterological Association and has been published in abstract form
(McCole, D. F., Keely, S. J., and Barrett, K. E. (2001)
Gastroenterology 120, A526-A527 (Abstr.
2679)).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Finzi, M. X. G. Shao, F. Paye, C. Housset, and J. A. Nadel Lipopolysaccharide Initiates a Positive Feedback of Epidermal Growth Factor Receptor Signaling by Prostaglandin E2 in Human Biliary Carcinoma Cells J. Immunol., February 15, 2009; 182(4): 2269 - 2276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zou, S. M. Thomas, Z.-W. Yan, J. R. Grandis, A. Vogt, and L.-Y. Li Human rhomboid family-1 gene RHBDF1 participates in GPCR-mediated transactivation of EGFR growth signals in head and neck squamous cancer cells FASEB J, February 1, 2009; 23(2): 425 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Chappell, M. Bunz, E. Smoll, H. Dong, C. Lytle, K. E. Barrett, and D. F. McCole Hydrogen peroxide inhibits Ca2+-dependent chloride secretion across colonic epithelial cells via distinct kinase signaling pathways and ion transport proteins FASEB J, June 1, 2008; 22(6): 2023 - 2036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Barrett New ways of thinking about (and teaching about) intestinal epithelial function Advan Physiol Educ, March 1, 2008; 32(1): 25 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Q. van der Merwe, M. D. Hollenberg, and W. K. MacNaughton EGF receptor transactivation and MAP kinase mediate proteinase-activated receptor-2-induced chloride secretion in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G441 - G451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Reynolds, A. Parris, L. A. Evans, S. Lindqvist, P. Sharp, M. Lewis, R. Tighe, and M. R. Williams Dynamic and differential regulation of NKCC1 by calcium and cAMP in the native human colonic epithelium J. Physiol., July 15, 2007; 582(2): 507 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Huang, J. Hu, X. Bian, K. Chen, W. Gong, N. M. Dunlop, O.M. Z. Howard, and J. M. Wang Transactivation of the Epidermal Growth Factor Receptor by Formylpeptide Receptor Exacerbates the Malignant Behavior of Human Glioblastoma Cells Cancer Res., June 15, 2007; 67(12): 5906 - 5913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. F. McCole, A. Truong, M. Bunz, and K. E. Barrett Consequences of Direct Versus Indirect Activation of Epidermal Growth Factor Receptor in Intestinal Epithelial Cells Are Dictated by Protein-tyrosine Phosphatase 1B J. Biol. Chem., May 4, 2007; 282(18): 13303 - 13315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, J.-K. Chen, J. R. Falck, S. Anjaiah, J. H. Capdevila, and R. C. Harris Mitogenic Activity and Signaling Mechanism of 2-(14,15- Epoxyeicosatrienoyl)Glycerol, a Novel Cytochrome P450 Arachidonate Metabolite Mol. Cell. Biol., April 15, 2007; 27(8): 3023 - 3034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. C. Chow and K. E. Barrett Role of protein phosphatase 2A in calcium-dependent chloride secretion by human colonic epithelial cells Am J Physiol Cell Physiol, January 1, 2007; 292(1): C452 - C459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, R. R. Hodges, C. Funaki, D. Zoukhri, R. J. Gaivin, D. M. Perez, and D. A. Dartt Effects of {alpha}1D-adrenergic receptors on shedding of biologically active EGF in freshly isolated lacrimal gland epithelial cells Am J Physiol Cell Physiol, November 1, 2006; 291(5): C946 - C956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. V. Mukhin, M. Gooz, J. R. Raymond, and M. N. Garnovskaya Collagenase-2 and -3 Mediate Epidermal Growth Factor Receptor Transactivation by Bradykinin B2 Receptor in Kidney Cells J. Pharmacol. Exp. Ther., September 1, 2006; 318(3): 1033 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ohtsu, P. J. Dempsey, and S. Eguchi ADAMs as mediators of EGF receptor transactivation by G protein-coupled receptors Am J Physiol Cell Physiol, July 1, 2006; 291(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang, S. M. Thomas, V. W. Y. Lui, S. Xi, J. M. Siegfried, H. Fan, T. E. Smithgall, G. B. Mills, and J. R. Grandis Phosphorylation of TNF-{alpha} converting enzyme by gastrin-releasing peptide induces amphiregulin release and EGF receptor activation PNAS, May 2, 2006; 103(18): 6901 - 6906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Myhre, M. Toruner, S. Abraham, and L. J. Egan Metalloprotease disintegrin-mediated ectodomain shedding of EGFR ligands promotes intestinal epithelial restitution Am J Physiol Gastrointest Liver Physiol, December 1, 2004; 287(6): G1213 - G1219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Vazquez, B. J. Wedel, B. T. Kawasaki, G. St. J. Bird, and J. W. Putney Jr. Obligatory Role of Src Kinase in the Signaling Mechanism for TRPC3 Cation Channels J. Biol. Chem., September 24, 2004; 279(39): 40521 - 40528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhang, S. M. Thomas, S. Xi, T. E. Smithgall, J. M. Siegfried, J. Kamens, W. E. Gooding, and J. R. Grandis Src Family Kinases Mediate Epidermal Growth Factor Receptor Ligand Cleavage, Proliferation, and Invasion of Head and Neck Cancer Cells Cancer Res., September 1, 2004; 64(17): 6166 - 6173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Darmoul, V. Gratio, H. Devaud, F. Peiretti, and M. Laburthe Activation of Proteinase-Activated Receptor 1 Promotes Human Colon Cancer Cell Proliferation Through Epidermal Growth Factor Receptor Transactivation Mol. Cancer Res., September 1, 2004; 2(9): 514 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Tegeder and G. Geisslinger Opioids As Modulators of Cell Death and Survival--Unraveling Mechanisms and Revealing New Indications Pharmacol. Rev., September 1, 2004; 56(3): 351 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Argast, J. S. Campbell, J. T. Brooling, and N. Fausto Epidermal Growth Factor Receptor Transactivation Mediates Tumor Necrosis Factor-induced Hepatocyte Replication J. Biol. Chem., August 13, 2004; 279(33): 34530 - 34536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. X. G. Shao, T. Nakanaga, and J. A. Nadel Cigarette smoke induces MUC5AC mucin overproduction via tumor necrosis factor-{alpha}-converting enzyme in human airway epithelial (NCI-H292) cells Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L420 - L427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang, Y. Chang, X. Wang, J. Jiang, and S. J. Frank Growth Hormone Alters Epidermal Growth Factor Receptor Binding Affinity via Activation of Extracellular Signal-Regulated Kinases in 3T3-F442A Cells Endocrinology, July 1, 2004; 145(7): 3297 - 3306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Hinkle, S. W. Sunnarborg, D. Loiselle, C. E. Parker, M. Stevenson, W. E. Russell, and D. C. Lee Selective Roles for Tumor Necrosis Factor {alpha}-converting Enzyme/ADAM17 in the Shedding of the Epidermal Growth Factor Receptor Ligand Family: THE JUXTAMEMBRANE STALK DETERMINES CLEAVAGE EFFICIENCY J. Biol. Chem., June 4, 2004; 279(23): 24179 - 24188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Darmoul, V. Gratio, H. Devaud, and M. Laburthe Protease-activated Receptor 2 in Colon Cancer: TRYPSIN-INDUCED MAPK PHOSPHORYLATION AND CELL PROLIFERATION ARE MEDIATED BY EPIDERMAL GROWTH FACTOR RECEPTOR TRANSACTIVATION J. Biol. Chem., May 14, 2004; 279(20): 20927 - 20934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, H. Zhang, A. Cheong, S. Leu, Y. Chen, C. G. Elowsky, and M. Donowitz Carbachol regulation of rabbit ileal brush border Na+-H+ exchanger 3 (NHE3) occurs through changes in NHE3 trafficking and complex formation and is Src dependent J. Physiol., May 1, 2004; 556(3): 791 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wu, J. M. Samet, R. Silbajoris, L. A. Dailey, D. Sheppard, P. A. Bromberg, and L. M. Graves Heparin-Binding Epidermal Growth Factor Cleavage Mediates Zinc-Induced Epidermal Growth Factor Receptor Phosphorylation Am. J. Respir. Cell Mol. Biol., April 1, 2004; 30(4): 540 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Bertelsen, K. E. Barrett, and S. J. Keely Gs Protein-coupled Receptor Agonists Induce Transactivation of the Epidermal Growth Factor Receptor in T84 Cells: IMPLICATIONS FOR EPITHELIAL SECRETORY RESPONSES J. Biol. Chem., February 20, 2004; 279(8): 6271 - 6279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Egan, A. de Lecea, E. D. Lehrman, G. M. Myhre, L. Eckmann, and M. F. Kagnoff Nuclear factor-{kappa}B activation promotes restitution of wounded intestinal epithelial monolayers Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1028 - C1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. B. Vargaftig and M. Singer Leukotrienes mediate part of Ova-induced lung effects in mice via EGFR Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L808 - L818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Werneburg, J.-H. Yoon, H. Higuchi, and G. J. Gores Bile acids activate EGF receptor via a TGF-{alpha}-dependent mechanism in human cholangiocyte cell lines Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G31 - G36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Huang, S.-O. Kim, J. Jiang, and S. J. Frank Growth Hormone-induced Phosphorylation of Epidermal Growth Factor (EGF) Receptor in 3T3-F442A Cells: MODULATION OF EGF-INDUCED TRAFFICKING AND SIGNALING J. Biol. Chem., May 23, 2003; 278(21): 18902 - 18913. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |