JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M207601200 on August 28, 2002

J. Biol. Chem., Vol. 277, Issue 45, 42781-42789, November 8, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/45/42781    most recent
M207601200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Panzenboeck, U.
Right arrow Articles by Sattler, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Panzenboeck, U.
Right arrow Articles by Sattler, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ABCA1 and Scavenger Receptor Class B, Type I, Are Modulators of Reverse Sterol Transport at an in Vitro Blood-Brain Barrier Constituted of Porcine Brain Capillary Endothelial Cells*

Ute Panzenboeck, Zoltan Balazs, Andrea Sovic, Andelko Hrzenjak, Sanja Levak-Frank, Andrea Wintersperger, Ernst Malle, and Wolfgang SattlerDagger

From the Institute of Medical Biochemistry and Medical Molecular Biology, University Graz, Harrachgasse 21, A-8010 Graz, Austria

The objective of the present study was to investigate the involvement of key players in reverse cholesterol/24(S)OH-cholesterol transport in primary porcine brain capillary endothelial cells (pBCEC) that constitute the BBB. We identified that, in addition to scavenger receptor class B, type I (SR-BI), pBCEC express ABCA1 and apolipoprotein A-I (apoA-I) mRNA and protein. Studies on the regulation of ABCA1 by the liver X receptor agonist 24(S)OH-cholesterol revealed increased ABCA1 expression and apoA-I-dependent [3H]cholesterol efflux from pBCEC. In unpolarized pBCEC, high density lipoprotein, subclass 3 (HDL3)-dependent [3H]cholesterol efflux, was unaffected by 24(S)OH-cholesterol treatment but was enhanced 5-fold in SR-BI overexpressing pBCEC. Efflux of cellular 24(S)-[3H]OH-cholesterol was highly efficient, independent of ABCA1, and correlated with SR-BI expression. Polarized pBCEC were cultured on porous membrane filters that allow separate access to the apical and the basolateral compartment. Addition of cholesterol acceptors to the apical compartment resulted in preferential [3H]cholesterol efflux to the basolateral compartment. HDL3 was a better promoter of basolateral [3H]cholesterol efflux than lipid-free apoA-I. Basolateral pretreatment with 24(S)OH-cholesterol enhanced apoA-I-dependent basolateral cholesterol efflux up to 2-fold along with the induction of ABCA1 at the basolateral membrane. Secretion of apoA-I also occurred preferentially to the basolateral compartment, where the majority of apoA-I was recovered in an HDL-like density range. In contrast, 24(S)-[3H]OH-cholesterol was mobilized efficiently to the apical compartment of the in vitro BBB by HDL3, low density lipoprotein, and serum. These results suggest the existence of an autoregulatory mechanism for removal of potentially neurotoxic 24(S)OH-cholesterol. In conclusion, the apoA-I/ABCA1- and HDL/SR-BI-dependent pathways modulate polarized sterol mobilization at the BBB.

* This work was supported by the Austrian National Bank Grants OENB 9622 (to W. S.) and 8778 (to E. M.) and the Austrian Science Foundation (FWF) Grants SFB 007-716 (to W. S.), P14186-MED, and P15404-MED (to E. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Institute of Medical Biochemistry and Medical Molecular Biology, University Graz, Harrachgasse 21, 8010 Graz, Austria. Tel.: 43-316-380-4188; Fax: 43-316-380-9615; E-mail: wolfgang.sattler@kfunigraz.ac.at.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
A. Lyly, S. K. Marjavaara, A. Kyttala, K. Uusi-Rauva, K. Luiro, O. Kopra, L. O. Martinez, K. Tanhuanpaa, N. Kalkkinen, A. Suomalainen, et al.
Deficiency of the INCL protein Ppt1 results in changes in ectopic F1-ATP synthase and altered cholesterol metabolism
Hum. Mol. Genet., May 15, 2008; 17(10): 1406 - 1417.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
U. Panzenboeck, U. Andersson, M. Hansson, W. Sattler, S. Meaney, and I. Bjorkhem
On the mechanism of cerebral accumulation of cholestanol in patients with cerebrotendinous xanthomatosis
J. Lipid Res., May 1, 2007; 48(5): 1167 - 1174.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
M. Guizzetti and L. Costa
Cholesterol homeostasis in the developing brain: a possible new target for ethanol
Human and Experimental Toxicology, April 1, 2007; 26(4): 355 - 360.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
B. Karten, R. B. Campenot, D. E. Vance, and J. E. Vance
Expression of ABCG1, but Not ABCA1, Correlates with Cholesterol Release by Cerebellar Astroglia
J. Biol. Chem., February 17, 2006; 281(7): 4049 - 4057.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y. Zhu, H. Liao, X. Xie, Y. Yuan, T.-S. Lee, N. Wang, X. Wang, J. Y.-J. Shyy, and M. B. Stemerman
Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting liver X receptor (LXR)
Cardiovasc Res, December 1, 2005; 68(3): 425 - 432.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Rigamonti, L. Helin, S. Lestavel, A.L. Mutka, M. Lepore, C. Fontaine, M.A. Bouhlel, S. Bultel, J.C. Fruchart, E. Ikonen, et al.
Liver X Receptor Activation Controls Intracellular Cholesterol Trafficking and Esterification in Human Macrophages
Circ. Res., September 30, 2005; 97(7): 682 - 689.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J.-i. Ito, H. Li, Y. Nagayasu, A. Kheirollah, and S. Yokoyama
Apolipoprotein A-I induces translocation of protein kinase C{alpha} to a cytosolic lipid-protein particle in astrocytes
J. Lipid Res., December 1, 2004; 45(12): 2269 - 2276.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Zeng, H. Liao, Y. Liu, T.-S. Lee, M. Zhu, X. Wang, M. B. Stemerman, Y. Zhu, and J. Y.-J. Shyy
Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM
J. Biol. Chem., November 19, 2004; 279(47): 48801 - 48807.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
B. J. O'Connell, M. Denis, and J. Genest
Cellular Physiology of Cholesterol Efflux in Vascular Endothelial Cells
Circulation, November 2, 2004; 110(18): 2881 - 2888.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
I. Bjorkhem and S. Meaney
Brain Cholesterol: Long Secret Life Behind a Barrier
Arterioscler. Thromb. Vasc. Biol., May 1, 2004; 24(5): 806 - 815.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. F. Oram
HDL Apolipoproteins and ABCA1: Partners in the Removal of Excess Cellular Cholesterol
Arterioscler. Thromb. Vasc. Biol., May 1, 2003; 23(5): 720 - 727.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. P. Koldamova, I. M. Lefterov, M. D. Ikonomovic, J. Skoko, P. I. Lefterov, B. A. Isanski, S. T. DeKosky, and J. S. Lazo
22R-Hydroxycholesterol and 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression and Cholesterol Efflux in Brain Cells and Decrease Amyloid beta Secretion
J. Biol. Chem., April 4, 2003; 278(15): 13244 - 13256.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
V. Leoni, T. Masterman, P. Patel, S. Meaney, U. Diczfalusy, and I. Bjorkhem
Side chain oxidized oxysterols in cerebrospinal fluid and the integrity of blood-brain and blood-cerebrospinal fluid barriers
J. Lipid Res., April 1, 2003; 44(4): 793 - 799.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. M. Dietschy and S. D. Turley
Thematic review series: Brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal
J. Lipid Res., August 1, 2004; 45(8): 1375 - 1397.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.