|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 45, 43359-43368, November 8, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The newly discovered p53 family member, p73, has
a striking homology to p53 in both sequence and modular structure.
Ectopic expression of p73 promotes transcription of p53 target genes
and recapitulates the most characterized p53 biological effects such as
growth arrest, apoptosis, and differentiation. Unlike p53-deficient mice that develop normally but are subject to spontaneous tumor formation, p73-deficient mice exhibit severe defects in the development of central nervous system and suffer from inflammation but are not
prone to tumor development. These phenotypes suggest different biological activities mediated by p53 and p73 that might reflect activation of specific sets of target genes. Here, we have analyzed the
gene expression profile of H1299 cells after p73
Identification of Direct p73 Target Genes Combining DNA
Microarray and Chromatin Immunoprecipitation Analyses*
§,
,,,,¶¶
Department of Experimental Oncology, Regina
Elena Cancer Institute, Rome 00158, Italy, ¶ Department of Physics
of Complex System Weizmann Institute of Science, Rehovot 76100, Israel,
** Department of Pediatric Hemato-Oncology, The Chaim Sheba
Medical Center and Sackler School of Medicine, Tel-Aviv University,
Israel, and §§ Department of Molecular Cell
Biology, Weizmann Institute of Science, Rehovot 76100, Israel
or p53 activation using oligonucleotide microarrays capable of detecting ~11,000 mRNA species. Our results indicate that p73 and p53 activate both common and distinct groups of genes. We found 141 and 320 genes
whose expression is modulated by p73 and p53,
respectively. p73 up-regulates 85 genes, whereas p53 induces 153 genes, of which 27 are in common with p73. Functional classification
of these genes reveals that they are involved in many aspects of cell
function ranging from cell cycle and apoptosis to DNA repair. Furthermore, we report that some of the up-regulated genes are directly
activated by p73 or p53.
*
This work was supported in part by Yad Abraham Research
Center for Cancer Diagnosis and Therapy at the Weizmann Institute, the
Irving Green Alzheimer research fund, Italian Association for Cancer
Research, Italian National Research Council, Italian Health Office, and
by European Community Grant QLG1-1999-00273.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Partially supported by the German-Israel Science Foundation
and the Israel Science Foundation.
Holds the Gregorio and Dora Shapiro Chair for Hematologic
Malignancies, Sackler School of Medicine, Tel Aviv University.
¶¶
To whom correspondence should be addressed: Dept. of
Experimental Oncology, Regina Elena Cancer Institute, Via delle Messi d'oro, 156, 00158-Rome, Italy. Tel.: 39-06-52662522; Fax:
39-06-4180526; E-mail: blandino@ifo.it.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Rosenbluth, D. J. Mays, M. F. Pino, L. J. Tang, and J. A. Pietenpol A Gene Signature-Based Approach Identifies mTOR as a Regulator of p73 Mol. Cell. Biol., October 1, 2008; 28(19): 5951 - 5964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tozluoglu, E. Karaca, T. Haliloglu, and R. Nussinov Cataloging and organizing p73 interactions in cell cycle arrest and apoptosis Nucleic Acids Res., September 1, 2008; 36(15): 5033 - 5049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sasaki, Y. Oshima, R. Koyama, R. Maruyama, H. Akashi, H. Mita, M. Toyota, Y. Shinomura, K. Imai, and T. Tokino Identification of Flotillin-2, a Major Protein on Lipid Rafts, as a Novel Target of p53 Family Members Mol. Cancer Res., March 1, 2008; 6(3): 395 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jing, J. Bohl, N. Brimer, M. Kinter, and S. B. Vande Pol Degradation of Tyrosine Phosphatase PTPN3 (PTPH1) by Association with Oncogenic Human Papillomavirus E6 Proteins J. Virol., March 1, 2007; 81(5): 2231 - 2239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lokshin, Y. Li, C. Gaiddon, and C. Prives p53 and p73 display common and distinct requirements for sequence specific binding to DNA Nucleic Acids Res., January 12, 2007; 35(1): 340 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dicker, A. P. Kater, C. E. Prada, T. Fukuda, J. E. Castro, G. Sun, J. Y. Wang, and T. J. Kipps CD154 induces p73 to overcome the resistance to apoptosis of chronic lymphocytic leukemia cells lacking functional p53 Blood, November 15, 2006; 108(10): 3450 - 3457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Klochendler-Yeivin, E. Picarsky, and M. Yaniv Increased DNA Damage Sensitivity and Apoptosis in Cells Lacking the Snf5/Ini1 Subunit of the SWI/SNF Chromatin Remodeling Complex. Mol. Cell. Biol., April 1, 2006; 26(7): 2661 - 2674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jain, S. Gupta, Ch. Sudhakar, V. Radha, and G. Swarup Role of p73 in Regulating Human Caspase-1 Gene Transcription Induced by Interferon-{gamma} and Cisplatin J. Biol. Chem., November 4, 2005; 280(44): 36664 - 36673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Stephens, H. Han, V. Gokhale, and D. D. Von Hoff PRL phosphatases as potential molecular targets in cancer Mol. Cancer Ther., November 1, 2005; 4(11): 1653 - 1661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Merlo, M. Fulco, A. Costanzo, R. Mangiacasale, S. Strano, G. Blandino, Y. Taya, P. Lavia, and M. Levrero A Role of p73 in Mitotic Exit J. Biol. Chem., August 26, 2005; 280(34): 30354 - 30360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Saifudeen, V. Diavolitsis, J. Stefkova, S. Dipp, H. Fan, and S. S. El-Dahr Spatiotemporal Switch from {Delta}Np73 to TAp73 Isoforms during Nephrogenesis: IMPACT ON DIFFERENTIATION GENE EXPRESSION J. Biol. Chem., June 17, 2005; 280(24): 23094 - 23102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Li, J. Zhu, and J. Y. J. Wang Ectopic Expression of p73{alpha}, but Not p73{beta}, Suppresses Myogenic Differentiation J. Biol. Chem., January 21, 2005; 280(3): 2159 - 2164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Howell, C. Borchers, and S. L. Milgram Heterogeneous Nuclear Ribonuclear Protein U Associates with YAP and Regulates Its Co-activation of Bax Transcription J. Biol. Chem., June 18, 2004; 279(25): 26300 - 26306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Billon, A. Terrinoni, C. Jolicoeur, A. McCarthy, W. D. Richardson, G. Melino, and M. Raff Roles for p53 and p73 during oligodendrocyte development Development, March 15, 2004; 131(6): 1211 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fulco, A. Costanzo, P. Merlo, R. Mangiacasale, S. Strano, G. Blandino, C. Balsano, P. Lavia, and M. Levrero p73 Is Regulated by Phosphorylation at the G2/M Transition J. Biol. Chem., December 5, 2003; 278(49): 49196 - 49202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sasaki, H. Mita, M. Toyota, S. Ishida, I. Morimoto, T. Yamashita, T. Tanaka, K. Imai, Y. Nakamura, and T. Tokino Identification of the Interleukin 4 Receptor {alpha} Gene as a Direct Target for p73 Cancer Res., December 1, 2003; 63(23): 8145 - 8152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Albertson and D. Pinkel Genomic microarrays in human genetic disease and cancer Hum. Mol. Genet., October 15, 2003; 12(90002): R145 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Sablina, P. M. Chumakov, and B. P. Kopnin Tumor Suppressor p53 and Its Homologue p73{alpha} Affect Cell Migration J. Biol. Chem., July 18, 2003; 278(30): 27362 - 27371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Merchant, L. Bai, and M. Okada ZBP-89 Mediates Butyrate Regulation of Gene Expression J. Nutr., July 1, 2003; 133(7): 2456S - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lotem, H. Gal, R. Kama, N. Amariglio, G. Rechavi, E. Domany, L. Sachs, and D. Givol Inhibition of p53-induced apoptosis without affecting expression of p53-regulated genes PNAS, May 27, 2003; 100(11): 6718 - 6723. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |