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J. Biol. Chem., Vol. 277, Issue 45, 43389-43398, November 8, 2002
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From the Biochemical and cell fractionation studies
suggest caveolae contain functionally organized sets of signaling
molecules that are capable of transmitting specific signals to the
cell. It is not known, however, whether any signals actually
originate from caveolae in living cells. To address this question, we
have engineered the calcium sensor yellow cameleon so that it is
targeted either to the plasma membrane, caveolae, or the cytoplasm of
endothelial cells. Quantitative measurements of the three
Ca2+ pools detected by these probes indicate that
caveolae are preferred sites of Ca2+ entry when
Ca2+ stores in the endoplasmic reticulum are
depleted. These results suggest that the signaling machinery in
control of Ca2+ entry is functionally organized in the
caveolae of living cells.
A Molecular Sensor Detects Signal Transduction
from Caveolae in Living Cells*
,,¶
Department of Cell Biology, University of
Texas Southwestern Medical Center, Dallas, Texas 75235-9039 and the
§ Department of Nephrology and Endocrinology, University of
Tokyo, Tokyo 113-8688, Japan
*
This work was supported by Grants HL 20948 and GM 52016 from
the National Institutes of Health, the Perot Family Foundation, the
Japanese Heart Foundation Grant for Research on Hypertension and
Vascular Metabolism, and the Banyu Fellowship Awards in Cardiovascular Medicine, which is sponsored by Banyu Pharmaceutical Co., Ltd., and The
Merck Company Foundation.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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