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J. Biol. Chem., Vol. 277, Issue 45, 43399-43409, November 8, 2002
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From the Plasma membranes are organized into functional
domains both by liquid-ordered packing into "lipid rafts,"
structures that resist Triton extraction, and by attachments to
underlying cytoskeletal proteins in assemblies called "membrane
skeletons." Although the actin cytoskeleton is implicated in many
lipid raft-mediated signaling processes, little is known about the
biochemical basis for actin involvement. We show here that a subset of
plasma membrane skeleton proteins from bovine neutrophils co-isolates
with cholesterol-rich, detergent-resistant membrane fragments (DRMs)
that exhibit a relatively high buoyant density in sucrose (DRM-H;
d ~1.16 g/ml). By using matrix-assisted laser
desorption/ionization time of flight and tandem mass spectrometry, we
identified 19 major DRM-H proteins. Membrane skeleton proteins include
fodrin (nonerythroid spectrin), myosin-IIA, myosin-IG,
Proteomic Analysis of a Detergent-resistant Membrane Skeleton
from Neutrophil Plasma Membranes*,
,§,,, and
Department of Cell Biology, University of
Massachusetts Medical School, Worcester, Massachusetts 01605 and
¶ Proteomic Mass Spectrometry Laboratory, University of
Massachusetts Medical School, Shrewsbury, Massachusetts 01545
-actinin 1, -actinin 4, vimentin, and the F-actin-binding protein, supervillin.
Other DRM-H components include lipid raft-associated integral membrane
proteins (stomatin, flotillin 1, and flotillin 2), extracellular
surface-bound and glycophosphatidylinositol-anchored proteins (IgM,
membrane-type 6 matrix metalloproteinase), and intracellular dually
acylated signaling proteins (Lyn kinase, Gi-2).
Consistent with cytoskeletal association, most DRM-H-associated
flotillin 2, Lyn, and Gi-2 also resist extraction with
0.1 M octyl glucoside. Supervillin, myosin-IG, and
myosin-IIA resist extraction with 0.1 M sodium carbonate, a
treatment that removes all detectable actin, suggesting that these
cytoskeletal proteins are proximal to the DRM-H bilayer. Binding of
supervillin to the DRM-H fragments is confirmed by co-immunoaffinity
purification. In spreading neutrophils, supervillin localizes with
F-actin in cell extensions and in discrete basal puncta that partially
overlap with Gi staining. We suggest that the DRM-H
fraction represents a membrane skeleton-associated subset of leukocyte
signaling domains.
*
This work was supported by Grant GM33048 from the National
Institutes of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
The on-line version of this article (available at
http://www.jbc.org) contains information for Fig. 4.
§
Present address: Scripps Research Institute, 10550 N. Torrey Pines
Rd., La Jolla, CA 92037.
To whom correspondence should be addressed: Dept. of Cell
Biology, University of Massachusetts Medical School, Biotech 4, Ste.
306, 377 Plantation St., Worcester, MA 01605. Tel.: 508-856-8661; Fax:
508-856-8774; E-mail: Elizabeth.Luna@umassmed.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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