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J. Biol. Chem., Vol. 277, Issue 46, 44131-44139, November 15, 2002
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From the Division of Food Safety Science, Institute of Food
Research, Norwich Research Park, Colney,
Norwich NR4 7UA, United Kingdom
S-Adenosylmethionine
decarboxylase (AdoMetDC) is a key enzyme in polyamine biosynthesis. We
show that the plant AdoMetDC activity is subject to
post-transcriptional control by polyamines. A highly conserved small
upstream open reading frame (uORF) in the AdoMetDC mRNA 5' leader
is responsible for translational repression of a downstream
Abrogation of Upstream Open Reading Frame-mediated
Translational Control of a Plant S-Adenosylmethionine
Decarboxylase Results in Polyamine Disruption and Growth
Perturbations*
-glucuronidase reporter cistron in transgenic tobacco plants.
Elimination of the small uORF from an AdoMetDC cDNA led to
increased relative translational efficiency of the AdoMetDC proenzyme
in transgenic plants. The resulting increased activity of AdoMetDC
caused disruption to polyamine levels with depletion of putrescine,
reduction of spermine levels, and a more than 400-fold increase in the
level of decarboxylated S-adenosylmethionine. These changes
were associated with severe growth and developmental defects. The high
level of decarboxylated S-adenosylmethionine was not
associated with any change in 5'-methylcytosine content in genomic DNA
and S-adenosylmethionine levels were more or less normal,
indicating a highly efficient system for maintenance of S-adenosylmethionine levels in plants. This work
demonstrates that uORF-mediated translational control of AdoMetDC is
essential for polyamine homeostasis and for normal growth and development.
*
This work was supported in part by the Foods Standards
Agency.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
To whom correspondence should be addressed: Div. of Food Safety
Science, Institute of Food Research, Norwich Research Park, Colney,
Norwich NR4 7UA, United Kingdom. Tel.: 44-1603-255356; Fax:
44-1603-507723; E-mail: tony.michael@bbsrc.ac.uk.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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