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J. Biol. Chem., Vol. 277, Issue 47, 45034-45040, November 22, 2002
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From the This report describes the identification of
a novel protein named PS1D (GenbankTM
accession number AJ272345), which is composed of an S1-like RNA-binding domain, a (cysteine)×3-(histidine) CCCH-zinc finger, and a
very basic carboxyl domain. PS1D is expressed as two isoforms, probably
resulting from the alternative splicing of mRNA. The long PS1D
isoform differs from the short one by the presence of 48 additional
amino acids at its amino-terminal extremity. Analysis of PS1D
subcellular distribution by cell fractionation reveals that this
protein belongs to the core of the eukaryotic 60S ribosomal subunit.
Interestingly, PS1D protein is a highly conserved protein among
mammalians as murine, human, and simian PS1D homologues share more than
95% identity. In contrast, no homologous protein is found in lower
eukaryotes such as yeast and Caenorhabditis elegans. These
observations indicate that PS1D is the first eukaryotic ribosomal
protein that is specific to higher eukaryotes.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ272345.
Identification of Ribosomal Proteins Specific to
Higher Eukaryotic Organisms*
§,
,
, and
Laboratoire de Chimie Biologique and the
¶ Laboratoire de Biologie du Développement, Institut de
Biologie et de Médecine Moléculaires, Université
Libre de Bruxelles, 12 rue des Profs. Jeener et Brachet,
6041 Gosselies, Belgium
*
This work was funded by the European Community (Contract
QLK3-2000-00721), the Fund for Medical Scientific Research (Belgium, Grant 3.4618.01), and the Actions de Recherches Concertées (Grant 00-05/250).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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