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Originally published In Press as doi:10.1074/jbc.M207669200 on September 11, 2002

J. Biol. Chem., Vol. 277, Issue 47, 45235-45242, November 22, 2002
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The Shq1p·Naf1p Complex Is Required for Box H/ACA Small Nucleolar Ribonucleoprotein Particle Biogenesis*

Pok Kwan YangDagger , Giuseppe Rotondo§, Tanya PorrasDagger , Pierre Legrain§||, and Guillaume ChanfreauDagger **

From the Dagger  Department of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Los Angeles, California 90095-1569 and § GIM-Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France

Small nucleolar ribonucleoprotein particles (snoRNPs) are essential cofactors in ribosomal RNA metabolism. Although snoRNP composition has been thoroughly characterized, the biogenesis process of these particles is poorly understood. We have identified two proteins from the yeast Saccharomyces cerevisiae, Yil104c/Shq1p and Ynl124w/Naf1p, which are essential and required for the stability of box H/ACA snoRNPs. Depletion of either Shq1p or Naf1p leads to a dramatic and specific decrease in box H/ACA snoRNA levels in vivo. A severe concomitant defect in ribosomal RNA processing is observed, consistent with the depletion of this family of snoRNAs. Shq1p and Naf1p show nuclear localization and interact with Nhp2p and Cbf5p, two core proteins of mature box H/ACA snoRNPs. Shq1p and Naf1p form a complex, but they are not strongly associated with box H/ACA snoRNPs. We propose that Shq1p and Naf1p are involved in the early biogenesis steps of box H/ACA snoRNP assembly.

* This work was supported by National Institutes of Health Grant GM61518 and by a Research Corp. Research Innovation Award (to G. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Present address: Johns Hopkins University School of Medicine, School of Medicine, 725 N. Wolfe St., 522 PCTB, Baltimore, MD 21205.

|| Present address: Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France.

** To whom correspondence should be addressed: Dept. of Chemistry and Biochemistry and the Molecular Biology Institute, UCLA, Box 951569, Los Angeles, CA 90095-1569. Tel.: 310-825-4399; Fax: 310-206-4038; E-mail: guillom@chem.ucla.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.