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J. Biol. Chem., Vol. 277, Issue 48, 46463-46469, November 29, 2002

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Rat Encodes the Paralogous Gene Equivalent of the Human Histo-blood Group ABO Gene
ASSOCIATION WITH ANTIGEN EXPRESSION BY OVEREXPRESSION OF HUMAN ABO TRANSFERASE^*

Sadahiko Iwamoto^§, Maki Kumada, Toyomi Kamesaki, Hiroshi Okuda, Eiji Kajii, Takeshi Inagaki^¶, Daisuke Saikawa^¶, Kouichi Takeuchi^¶, Sigeo Ohkawara^¶, Riichi Takahashi, Shoji Ueda, Seiichiro Inoue^**, Kazunori Tahara^**, Yoji Hakamata^**, and Eiji Kobayashi^**

From the  Department of Legal Medicine and Human Genetics, Jichi Medical School, the ^¶ Department of Anatomy, Jichi Medical School, the  YS New Technology Institute, Inc., Tochigi 329-04, Japan and the ^** Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan

We cloned a rat ABO homologue and established human A- and B-transferase transgenic rats. A DNA fragment corresponding to exon 7 of the human ABO gene was amplified from Wistar rat genomic DNA and sequenced. Using the amplified fragments as a probe for Southern blotting, multiple hybridized bands appeared on both EcoRI- and BamHI-digested genomes of seven rat strains, which showed variations in the band numbers among the strains. Four cDNAs were cloned from a Wistar rat, three of which showed A-transferase activity and one of which showed B-transferase activity. These activities were dependent on the equivalent residues at 266 and 268 of human ABO transferase. Wild Wistar rats expressed A-antigen in salivary gland, intestine, and urinary bladder tissue, but B-antigen was not stained in any organs studied, whereas a transcript from the ABO homologue with B-transferase activity was ubiquitous. Human A-transferase and B-transferase were transferred into Wistar rats. A-transgenic rats expressed A-antigen in ectopic tissue of the brain plexus, type II lung epithelium, pancreas, and epidermis. B-antigen in the B-transgenic rat was expressed in the same organs as A-transgenic rats. These results may shed light on the function and evolution of the ABO gene in primates.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AB081649, AB081650, AB081651, and AB081652.

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