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Originally published In Press as doi:10.1074/jbc.M208576200 on September 25, 2002

J. Biol. Chem., Vol. 277, Issue 49, 46864-46870, December 6, 2002
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Complex Nuclear Localization Signals in the Matrix Protein of Vesicular Stomatitis Virus*

Doreen R. Glodowski, Jeannine M. PetersenDagger , and James E. Dahlberg§

From the Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706-1532

The matrix (M) protein of vesicular stomatitis virus (VSV) functions from within the nucleus to inhibit bi-directional nucleocytoplasmic transport. Here, we show that M protein can be imported into the nucleus by an active transport mechanism, even though it is small enough (~27 kDa) to diffuse through nuclear pore complexes. We map two distinct nuclear localization signal (NLS)-containing regions of M protein, each of which is capable of directing the nuclear localization of a heterologous protein. One of these regions, comprising amino acids 47-229, is also sufficient to inhibit nucleocytoplasmic transport. Two amino acids that are conserved among the matrix proteins of vesiculoviruses are important for nuclear localization, but are not essential for the inhibitory activity of M protein. Thus, different regions of M protein function for nuclear localization and for inhibitory activity.

* This work was supported by National Institutes of Health Grant GM-30220 (to J. E. D.) and a Burroughs Wellcome Fund of the Life Sciences Research Foundation fellowship (to J. M. P.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Present address: Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, Centers for Disease Control, 1300 Rampart Rd, Ft. Collins, CO 80521.

§ To whom correspondence should be addressed: Dept. of Biomolecular Chemistry, University of Wisconsin-Madison, 1300 University Ave., Madison, WI 53706-1532. Tel.: 608-262-1459; Fax: 608-262-8704; E-mail: dahlberg@facstaff.wisc.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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