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J. Biol. Chem., Vol. 277, Issue 49, 47671-47678, December 6, 2002
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From the The cellular prion protein
(PrPC) is a glycosylphosphatidylinositol
(GPI)-anchored protein. We investigated whether PrPC can
move from one cell to another cell in a cell model. Little PrPC transfer was detected when a PrPC
expressing human neuroblastoma cell line was cultured with the human
erythroleukemia cells IA lacking PrPC. Efficient transfer
of PrPC was detected with the presence of phorbol
12-myristate 13-acetate, an activator of protein kinase C. Maximum PrPC transfer was observed when both donor and
recipient cells were activated. Furthermore, PrPC transfer
required the GPI anchor and direct cell to cell contact. However,
intercellular protein transfer is not limited to PrPC,
another GPI-anchored protein, CD90, also transfers from the donor cells
to acceptor cells after cellular activation. Therefore, this transfer
process is GPI-anchor and cellular activation dependent. These findings
suggest that the intercellular transfer of GPI-anchored proteins is a
regulated process, and may have implications for the pathogenesis of
prion disease.
Intercellular Transfer of the Cellular Prion
Protein*
,,,,,§,, and
Division of Neuropathology, Institute of
Pathology, ¶ Cancer Research Center, School of Medicine, Case
Western Reserve University, Cleveland, Ohio 44106
*
This work was supported in part by National Institutes of
Health Grant AG14359 and a contract from the Prion Developmental Laboratory. The Confocal Microscopy Facility was supported by National Institutes of Health Grant PO30CA43703.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: BRB, Rm. 933, School of Medicine, Case Western Reserve University, 10900 Euclid Ave.,
Cleveland OH 44106. Tel.: 216-368-1268; Fax: 216-368-1357; E-mail:
mxs92@po.cwru.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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