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J. Biol. Chem., Vol. 277, Issue 49, 47686-47691, December 6, 2002

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The Rho GTPase Effector Protein, mDia, Inhibits the DNA Binding Ability of the Transcription Factor Pax6 and Changes the Pattern of Neurite Extension in Cerebellar Granule Cells through Its Binding to Pax6^*

Tomoko Tominaga^§¶, Wenxiang Meng, Kazuya Togashi, Hiroko Urano, and Makoto Tominaga

From the  Department of Physiology, Mie University School of Medicine, Tsu 514-8507, and the ^§ Foundation for Advancement of International Science, Tsukuba 305-0062, Japan

mDia, one of the target proteins of the GTPase Rho, is known to be involved in cytoskeletal reorganization and cytokinesis. Here, we report that mDia enters the nucleus and binds to the transcription factor, Pax6. In cultured non-neuronal cells, overexpression of mDia with Pax6 causes redistribution of some Pax6 molecules from the nucleus to the cytosol and decreases Pax6 transcriptional activity. Because Pax6 functions in the early central nervous system morphogenesis, we also examined the effects of mDia on endogenous Pax6 localization and neurite extension in cerebellar granule cells. Here too, Pax6 was partially mislocalized to the cytosol, and its expression level was decreased by mDia overexpression. In addition, mDia overexpression in these cells led to increased neurite branching and length. These results strongly suggest that mDia influences Pax6-induced transcriptional activity and axonal pathfinding in a way opposite from ROCK (Rho kinase) and that it may act via Pax6 to modulate early neuronal development.

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