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Originally published In Press as doi:10.1074/jbc.M209199200 on October 3, 2002

J. Biol. Chem., Vol. 277, Issue 49, 47756-47764, December 6, 2002
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In Vitro Fusion of Plant Golgi Membranes Can Be Influenced by Divalent Cations*

Yuichi TakedaDagger and Kunihiro Kasamo

From the Research Institute for Bioresources, Okayama University, 1-20-2 Chuo, Kurashiki 710-0046, Japan

The fusogenic activity of plant Golgi membranes was studied in a cell-free system by assaying lipid mixing and content leakages of fluorescence probes. Golgi membranes from mung bean (Vigna radiata L.) hypocotyl cells fused to liposomes in the absence of any cytosolic proteins and nucleotides. It was demonstrated that the fusion was mediated by integral membrane protein(s), and was influenced by divalent cations (mM). Mg2+, Ca2+, and Mn2+ ions enhanced the lipid mixing by reducing repulsive forces between membranes. In the content leakage assay, Mg2+ ions also showed a stimulative effect. However, other divalent cations were inhibitory. It is suggested that the fusion system of Golgi membranes comprises at least two components: one that mediates the formation of fusion intermediates prior to pore opening, and one that mediates the subsequent processes. The latter must be sensitive to divalent cations at millimolar concentrations. The fusion of Golgi and biological membranes was induced by divalent cations. We speculated about the biological role of the fusion system studied here.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Current address: Molecular Membrane Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Fax: 81-48-462-4679; E-mail: y-takeda@postman.riken.go.jp.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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