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J. Biol. Chem., Vol. 277, Issue 5, 3232-3235, February 1, 2002
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From the CNRS UMR 6061, Université de Rennes 1, Faculté
de Médecine, 2 Avenue Léon Bernard, 35043 Rennes Cedex,
France
In mammalian cells, certain mRNAs encoding
cytokines or proto-oncogenes are especially unstable, because of the
presence of a particular sequence element in their 3'-untranslated
region named ARE (A/U-rich element). AREs cause this instability by
provoking the rapid shortening of the poly(A) tail of the mRNA. The
deadenylation of mRNAs mediated by AREs containing repeats of the
AUUUA motif (class I/II AREs) is conserved in Xenopus
embryos. Here, we first extend these observations by showing that c-Jun
ARE, a representative of class III (non-AUUUA) AREs, also provokes the
deadenylation of a reporter RNA in Xenopus embryos. Next,
by immunodepletion and immunoneutralization experiments, we show that,
in Xenopus, the rapid deadenylation of RNAs that contain
the c-Jun ARE, but not an AUUUA ARE, requires EDEN-BP. This RNA-binding
protein was previously shown to provoke the rapid deadenylation of
certain Xenopus maternal RNAs. Finally, we show that
CUG-BP, the human homologue of EDEN-BP, specifically binds to c-Jun
ARE. Together, these results identify CUG-BP as a plausible
deadenylation factor responsible for the post-transcriptional control
of c-Jun proto-oncogene mRNA in mammalian cells.
c-Jun ARE Targets mRNA Deadenylation by an EDEN-BP (Embryo
Deadenylation Element-binding Protein)-dependent
Pathway*
,
*
This work was supported by grants from the European Union DG
XII Biotechnology Program, Ministère chargé de la recherche ACC-SV4, Association de Recherche contre le Cancer, and Ligue Nationale
contre le cancer. CNRS UMR 6061 is a component of IFR 97 Génomique Fonctionnelle et Santé.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 33 0 299 33 62 75; Fax: 33 0 299 33 62 00; E-mail:
Luc.Paillard@univ-rennes1.fr.
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