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J. Biol. Chem., Vol. 277, Issue 5, 3647-3657, February 1, 2002
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From the Overexpression of SSAT (polyamine catabolic
enzyme) in female mice results in impaired ovarian folliculogenesis and
uterine hypoplasia. To identify the molecular basis for this, the gene expression profiles in uterus and ovary and for comparison, liver and
kidney, from non-transgenic (NT) and SSAT transgenic (ST) mice were
compared. The mRNA abundance for lipoprotein lipase and
glyceraldehyde-3-phosphate dehydrogenase was elevated in all four ST
(>NT) tissues. The translation initiation factor-3 subunit 5 mRNA,
and transcripts related to endogenous murine
leukemia provirus (MLV-related) and
murine retrovirus-related
sequences (MuRRS) were decreased in ST tissues. A novel
calmodulin-related mRNA was strongly induced in ST liver and
kidney. SSAT overexpression was associated with increased levels of
IGF-binding protein-2 (IGFBP-2) in the uterus and ovary, and a
reduction in IGFBP-3 mRNA levels in the uterus. Exogenous
spermidine and spermine elevated endogenous IGFBP-2 and SSAT mRNA
abundance, whereas, putrescine stimulated IGFBP-2 mRNA abundance
and transfected IGFBP-2 gene promoter activity in human
(Hec-1-A) uterine cells. Sp1 and BTEB1 mRNAs that encode
transcription factors for the IGFBP-2 gene also were
induced in some ST tissues. The data suggest that SSAT and polyamines
are important for the control of molecular pathways underlying
reproductive tract tissue growth, phenotype, and function.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY061807-AY061811.
Altered Levels of Growth-related and Novel Gene Transcripts in
Reproductive and Other Tissues of Female Mice Overexpressing
Spermidine/Spermine N1-Acetyltransferase
(SSAT)*
,,
Interdisciplinary Concentration in Animal
Molecular and Cell Biology, Genetics Institute, and Department of
Animal Sciences, University of Florida, Gainesville, Florida
32611-0910, the ¶ Grace Cancer Drug Center, Roswell Park Cancer
Institute, Buffalo, New York 14263, and the § A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P. O. Box 1627, FIN-70211 Kuopio, Finland
*
This work was supported by National Institutes of Health
Grants HD21961 (to R. C. M. S. and F. A. S.),
CA76428 (to C. W. P. and J. J.), and CA-16056 (to
C. W. P.) and by the Florida Agricultural Experiment Station.
This is Florida Agricultural Experiment Station publication Series No.
R-08404.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be sent. Tel.: 352-392-5590;
Fax: 352-392-5595; E-mail: simmen@dps.ufl.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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