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J. Biol. Chem., Vol. 277, Issue 5, 3760-3766, February 1, 2002
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From the Department of Biology, York University, Toronto, Ontario
M3J 1P3, Canada
Many (+)-strand RNA viruses transcribe small
subgenomic (sg) mRNAs that allow for regulated expression of a
subset of their genes. Tomato bushy stunt virus (TBSV)
transcribes two such messages and here we report the identification of
a long-distance RNA·RNA interaction that is essential for the
efficient accumulation of capsid protein-encoding sg mRNA1. The
relevant base pairing interaction occurs within the TBSV RNA genome
between a 7-nucleotide (nt) long sequence, separated by just 3 nt from
the downstream sg mRNA1 initiation site, and a complementary
sequence positioned some ~1000 nt further upstream. Analyses of this
interaction indicate that it (i) functions in the (+)-strand, (ii)
modulates both (+)- and (
An RNA Activator of Subgenomic mRNA1 Transcription in Tomato
Bushy Stunt Virus*
)-strand sg mRNA1 accumulation, (iii)
specifically regulates the accumulation of sg mRNA1 ()-strands,
(iv) controls sg mRNA1 expression from an ectopic transcriptional
initiation site, (v) may occur in cis and, and (vi) could
nucleate the formation of a more complex RNA structure. These data are
most consistent with a role for this interaction in regulating sg
mRNA1 accumulation at the level of transcription.
*
This research was supported by grants from the Natural
Sciences and Engineering Research Council of Canada and a Premier's Research Excellence Award (to K. A. W.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Biology, York
University, 4700 Keele St., Toronto, Ontario M3J 1P3, Canada. Tel.:
416-736-5243; Fax: 416-736-5698; E-mail: kawhite@yorku.ca.
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