JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208960200 on September 24, 2002

J. Biol. Chem., Vol. 277, Issue 50, 48099-48106, December 13, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
277/50/48099    most recent
M208960200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Diaz, A. R.
Right arrow Articles by de Mendoza, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Diaz, A. R.
Right arrow Articles by de Mendoza, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Membrane Topology of the Acyl-Lipid Desaturase from Bacillus subtilis*,

Alejandra R. DiazDagger §, María C. MansillaDagger §||, Alejandro J. VilaDagger **Dagger Dagger , and Diego de MendozaDagger §§§

From the Dagger  Instituto de Biología Molecular y Celular de Rosario and Departamentos de § Microbiología and ** Química Biológica, Area Biofísica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina

The Bacillus subtilis acyl-lipid desaturase (Delta 5-Des) is an iron-dependent integral membrane protein, able to selectively introduce double bonds into long chain fatty acids. Structural information on membrane-bound desaturases is still limited, and the present topological information is restricted to hydropathy plots or sequence comparison with the evolutionary related alkane hydroxylase. The topology of Delta 5-Des was determined experimentally in Escherichia coli using a set of nine different fusions of N-terminal fragments of Delta 5-Des with the reporter alkaline phosphatase (Delta 5-Des-PhoA). The alkaline phosphatase activities of cells expressing the Delta 5-Des-PhoA fusions, combined with site-directed mutagenesis of His residues identified in most desaturases, suggest that a tripartite motif of His essential for catalysis is located on the cytoplasmic phase of the membrane. These data, together with surface Lys biotinylation experiments, support a model for Delta 5-Des as a polytopic membrane protein with six transmembrane- and one membrane-associated domain, which likely represents a substrate-binding motif. This study provides the first experimental evidence for the topology of a plasma membrane fatty acid desaturase. On the basis of our results and the presently available hydrophobicity profile of many acyl-lipid desaturases, we propose that these enzymes contain a new transmembrane domain that might play a critical role in the desaturation of fatty acids esterified in glycerolipids.

* This work was supported in part by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Agencia Nacional de Promoción Científica y Tecnológica.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Supplemental Material.

Supported by a predoctoral Fondo Para el Mejoramiento de la Calidad Universitaria (FOMEC) grant from the Departamento de Biología, Bioquímica y Farmacia de la Universidad Nacional del Sur.

|| Postdoctoral fellow from CONICET.

Dagger Dagger Career Investigator from CONICET and International Research Scholar of the Howard Hughes Medical Institute.

§§ Career Investigator from CONICET and International Research Scholar of the Howard Hughes Medical Institute. To whom correspondence should be addressed: Suipacha 531, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina. Tel.: 54-341-4350661; Fax: 54-341-4390465; E-mail: diegonet@citynet.net.ar.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Hoffmann, E. Hornung, S. Busch, N. Kassner, P. Ternes, G. H. Braus, and I. Feussner
A Small Membrane-peripheral Region Close to the Active Center Determines Regioselectivity of Membrane-bound Fatty Acid Desaturases from Aspergillus nidulans
J. Biol. Chem., September 14, 2007; 282(37): 26666 - 26674.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Meesapyodsuk, D. W. Reed, P. S. Covello, and X. Qiu
Primary Structure, Regioselectivity, and Evolution of the Membrane-bound Fatty Acid Desaturases of Claviceps purpurea
J. Biol. Chem., July 13, 2007; 282(28): 20191 - 20199.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
R. W. Ye, K. J. Stead, H. Yao, and H. He
Mutational and Functional Analysis of the {beta}-Carotene Ketolase Involved in the Production of Canthaxanthin and Astaxanthin
Appl. Envir. Microbiol., September 1, 2006; 72(9): 5829 - 5837.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. C. Man, M. Miyazaki, K. Chu, and J. M. Ntambi
Membrane Topology of Mouse Stearoyl-CoA Desaturase 1
J. Biol. Chem., January 13, 2006; 281(2): 1251 - 1260.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. E. Ciocchini, M. S. Roset, N. Inon de Iannino, and R. A. Ugalde
Membrane Topology Analysis of Cyclic Glucan Synthase, a Virulence Determinant of Brucella abortus
J. Bacteriol., November 1, 2004; 186(21): 7205 - 7213.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. C. Mansilla, L. E. Cybulski, D. Albanesi, and D. de Mendoza
Control of Membrane Lipid Fluidity by Molecular Thermosensors
J. Bacteriol., October 15, 2004; 186(20): 6681 - 6688.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Sasata, D. W. Reed, M. C. Loewen, and P. S. Covello
Domain Swapping Localizes the Structural Determinants of Regioselectivity in Membrane-bound Fatty Acid Desaturases of Caenorhabditis elegans
J. Biol. Chem., September 17, 2004; 279(38): 39296 - 39302.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. E. Cybulski, G. del Solar, P. O. Craig, M. Espinosa, and D. de Mendoza
Bacillus subtilis DesR Functions as a Phosphorylation-activated Switch to Control Membrane Lipid Fluidity
J. Biol. Chem., September 17, 2004; 279(38): 39340 - 39347.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Albanesi, M. C. Mansilla, and D. de Mendoza
The Membrane Fluidity Sensor DesK of Bacillus subtilis Controls the Signal Decay of Its Cognate Response Regulator
J. Bacteriol., May 1, 2004; 186(9): 2655 - 2663.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. G. Altabe, P. Aguilar, G. M. Caballero, and D. de Mendoza
The Bacillus subtilis Acyl Lipid Desaturase Is a {Delta}5 Desaturase
J. Bacteriol., May 15, 2003; 185(10): 3228 - 3231.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.