Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M206127200 on September 30, 2002

J. Biol. Chem., Vol. 277, Issue 50, 48333-48341, December 13, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/50/48333    most recent
M206127200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holmes, M. L.
Right arrow Articles by Chong, B. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holmes, M. L.
Right arrow Articles by Chong, B. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Cloning and Analysis of the Thrombopoietin-induced Megakaryocyte-specific Glycoprotein VI Promoter and Its Regulation by GATA-1, Fli-1, and Sp1*

Melissa L. HolmesDagger , Natalie BartleDagger , Michael EisbacherDagger , and Beng H. ChongDagger §

From the Dagger  Centre for Thrombosis and Vascular Research, and § Department of Medicine, St. George Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia

The exposure of collagen fibers at sites of vascular injury results in the adherence of platelets and their subsequent activation. The platelet collagen receptor glycoprotein (GP)1 VI plays a crucial role in platelet activation and thrombus formation and decreased levels or defective GPVI may lead to excessive bleeding. In addition, elevated levels of collagen receptors may predispose individuals to coronary heart disease or strokes. GPVI expression is restricted to platelets and their precursor cell, the megakaryocyte. In this study we investigate the regulation of GPVI expression and show that thrombopoietin induces its expression in the megakaryocytic cell line UT-7/TPO. A 5'-region flanking the transcription start point of the GPVI gene was cloned (-694 to +29) and we report that this putative GPVI promoter bestows megakaryocye-specific expression. Deletion analyses and site-directed mutagenesis identified Sp1227, GATA177, and Ets48 sites as essential for GPVI expression. We show that transcription factors GATA-1, Fli-1, and Sp1 can bind to and activate this promoter. Finally, GPVI mRNA was detected only in megakaryocytic cell lines expressing both Fli-1 and GATA-1, and we show that overexpression of Fli-1 in a stable cell line (which expresses endogenous GATA-1 and Sp1) results in expression of the endogenous GPVI gene.

* This work was supported by a grant from the Australian National Health and Medical Research Council and an infrastructure grant from the New South Wales Department of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF521646.

To whom correspondence should be addressed. Present address: Dept. of Medicine, St. George Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia. Tel.: 61-2-9350-2010; Fax: 61-2-9350-3998; E-mail: b.h.chong@unsw.edu.au.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
Z. Zou, A. A. Schmaier, L. Cheng, P. Mericko, S. K. Dickeson, T. P. Stricker, S. A. Santoro, and M. L. Kahn
Negative regulation of activated {alpha}2 integrins during thrombopoiesis
Blood, June 18, 2009; 113(25): 6428 - 6439.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. J. Stankiewicz and J. D. Crispino
ETS2 and ERG promote megakaryopoiesis and synergize with alterations in GATA-1 to immortalize hematopoietic progenitor cells
Blood, April 2, 2009; 113(14): 3337 - 3347.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sawada, C. Nishiyama, T. Kishi, T. Sasazuki, S. Komazawa-Sakon, X. Xue, J.-H. Piao, H. Ogata, J.-i. Nakayama, T. Taki, et al.
Fusion of OTT to BSAC Results in Aberrant Up-regulation of Transcriptional Activity
J. Biol. Chem., September 26, 2008; 283(39): 26820 - 26828.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Hazony, J. Lu, C. St. Hilaire, and K. Ravid
Hematopoietic gene promoters subjected to a group-combinatorial study of DNA samples: identification of a megakaryocytic selective DNA signature
Nucleic Acids Res., September 11, 2006; 34(16): 4416 - 4428.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
T. Ohmori, J. Mimuro, K. Takano, S. Madoiwa, Y. Kashiwakura, A. Ishiwata, M. Niimura, K. Mitomo, T. Tabata, M. Hasegawa, et al.
Efficient expression of a transgene in platelets using simian immunodeficiency virus-based vector harboring glycoprotein Ib{alpha} promoter: in vivo model for platelet-targeting gene therapy
FASEB J, July 1, 2006; 20(9): 1522 - 1524.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Fang, K. Hodivala-Dilke, B. D. Johnson, L. M. Du, R. O. Hynes, G. C. White II, and D. A. Wilcox
Therapeutic expression of the platelet-specific integrin, {alpha}IIb{beta}3, in a murine model for Glanzmann thrombasthenia
Blood, October 15, 2005; 106(8): 2671 - 2679.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. C. Hughan, Y. Senis, D. Best, A. Thomas, J. Frampton, P. Vyas, and S. P. Watson
Selective impairment of platelet activation to collagen in the absence of GATA1
Blood, June 1, 2005; 105(11): 4369 - 4376.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Kanaji, T. Kanaji, B. Jacquelin, M. Chang, D. J. Nugent, N. Komatsu, M. Moroi, K. Izuhara, and T. J. Kunicki
Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation
Blood, May 15, 2005; 105(10): 3888 - 3892.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. Hu, A. S. Philips, J. C. Kwok, M. Eisbacher, and B. H. Chong
Identification of Nuclear Import and Export Signals within Fli-1: Roles of the Nuclear Import Signals in Fli-1-Dependent Activation of Megakaryocyte-Specific Promoters
Mol. Cell. Biol., April 15, 2005; 25(8): 3087 - 3108.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Masuya, O. Moussa, T. Abe, T. Deguchi, T. Higuchi, Y. Ebihara, D. D. Spyropoulos, D. K. Watson, and M. Ogawa
Dysregulation of granulocyte, erythrocyte, and NK cell lineages in Fli-1 gene-targeted mice
Blood, January 1, 2005; 105(1): 95 - 102.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Jackers, G. Szalai, O. Moussa, and D. K. Watson
Ets-dependent Regulation of Target Gene Expression during Megakaryopoiesis
J. Biol. Chem., December 10, 2004; 279(50): 52183 - 52190.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Gottgens, C. Broccardo, M.-J. Sanchez, S. Deveaux, G. Murphy, J. R. Gothert, E. Kotsopoulou, S. Kinston, L. Delaney, S. Piltz, et al.
The scl +18/19 Stem Cell Enhancer Is Not Required for Hematopoiesis: Identification of a 5' Bifunctional Hematopoietic-Endothelial Enhancer Bound by Fli-1 and Elf-1
Mol. Cell. Biol., March 1, 2004; 24(5): 1870 - 1883.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement