JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208829200 on September 27, 2002

J. Biol. Chem., Vol. 277, Issue 50, 48745-48754, December 13, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/50/48745    most recent
M208829200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Misra, P.
Right arrow Articles by Reddy, J. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Misra, P.
Right arrow Articles by Reddy, J. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Phosphorylation of Transcriptional Coactivator Peroxisome Proliferator-activated Receptor (PPAR)-binding Protein (PBP)
STIMULATION OF TRANSCRIPTIONAL REGULATION BY MITOGEN-ACTIVATED PROTEIN KINASE*

Parimal MisraDagger §, Edward D. Owuor, Wenge Li, Songtao YuDagger , Chao QiDagger , Kirstin MeyerDagger , Yi-Jun ZhuDagger , M. Sambasiva RaoDagger , A.-N. Tony Kong, and Janardan K. ReddyDagger ||

From the Dagger  Department of Pathology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611-3008, the  Department of Pharmaceutics, College of Pharmacy, Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, New Jersey 08854-8020, and § Discovery Research, Dr. Reddy's Laboratories Ltd., Miyapur, Hyderabad 500050, India

Peroxisome proliferator-activated receptor (PPAR)-binding protein (PBP) is an important coactivator for PPARgamma and other transcription factors. PBP is an integral component of a multiprotein thyroid hormone receptor-associated protein (TRAP)/vitamin D3 receptor-interacting protein (DRIP)/activator-recruited cofactor (ARC) complex required for transcriptional activity. To study the regulation of PBP by cellular signaling pathways, we identified the phosphorylation sites of PBP. Using a combination of in vitro and in vivo approaches and mutagenesis of PBP phosphorylation sites, we identified six phosphorylation sites on PBP: one exclusive protein kinase A (PKA) phosphorylation site at serine 656, two protein kinase C (PKC) sites at serine 796 and serine 1345, a common PKA/PKC site at serine 756, and two extracellular signal-regulated kinase 2 sites of the mitogen-activated protein kinase (MAPK) family at threonine 1017 and threonine 1444. Binding of PBP to PPARgamma 1 or retinoid-X-receptor for 9-cis-retinoic acid (RXR) is independent of their phosphorylation states, implying no changes in protein-protein interaction after modification by phosphorylation. Overexpression of RafBXB, an activated upstream kinase of the MAPK signal transduction pathway, exerts a significant additive inductive effect on PBP coactivator function. This effect is significantly diminished by overexpression of RafBXB301, a dominant negative mutant of RafBXB. These results identify phosphorylation as a regulatory modification event of PBP and demonstrate that PBP phosphorylation by Raf/MEK/MAPK cascade exerts a positive effect on PBP coactivator function. The functional role of PKA and PKC phosphorylation sites in PBP remains to be elucidated.

* This work was supported by National Institutes of Health Grants GM23750 (to J. K. R.), CA84472 (to M. S. R.), KO8 ES00356 and CA-888898 (to Y.-J. Z.), and CA-73647 (to A.-N. T. K.) and by the Joseph L. Mayberry, Sr. Endowment Fund.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Dept. of Pathology, The Feinberg School of Medicine, Northwestern University, 303 E. Chicago Ave., Chicago, IL 60611-3008. Tel.: 312-503-8144; Fax: 312-503-8249; E-mail: jkreddy@northwestern.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
R. Gineste, A. Sirvent, R. Paumelle, S. Helleboid, A. Aquilina, R. Darteil, D. W. Hum, J.-C. Fruchart, and B. Staels
Phosphorylation of Farnesoid X Receptor by Protein Kinase C Promotes Its Transcriptional Activity
Mol. Endocrinol., November 1, 2008; 22(11): 2433 - 2447.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
N. Anderson and J. Borlak
Molecular Mechanisms and Therapeutic Targets in Steatosis and Steatohepatitis
Pharmacol. Rev., September 1, 2008; 60(3): 311 - 357.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Belakavadi, P. K. Pandey, R. Vijayvargia, and J. D. Fondell
MED1 Phosphorylation Promotes Its Association with Mediator: Implications for Nuclear Receptor Signaling
Mol. Cell. Biol., June 15, 2008; 28(12): 3932 - 3942.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Li, P. Gade, S. C. Nallar, A. Raha, S. K. Roy, S. Karra, J. K. Reddy, S. P. Reddy, and D. V. Kalvakolanu
The Med1 Subunit of Transcriptional Mediator Plays a Central Role in Regulating CCAAT/Enhancer-binding Protein-{beta}-driven Transcription in Response to Interferon-{gamma}
J. Biol. Chem., May 9, 2008; 283(19): 13077 - 13086.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H.-M. Zhang, L. Li, N. Papadopoulou, G. Hodgson, E. Evans, M. Galbraith, M. Dear, S. Vougier, J. Saxton, and P. E. Shaw
Mitogen-induced recruitment of ERK and MSK to SRE promoter complexes by ternary complex factor Elk-1
Nucleic Acids Res., May 1, 2008; 36(8): 2594 - 2607.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
W. Chen and R. G. Roeder
The Mediator subunit MED1/TRAP220 is required for optimal glucocorticoid receptor-mediated transcription activation
Nucleic Acids Res., September 25, 2007; 35(18): 6161 - 6169.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. K. Saxena, P. M. Vertino, F. A. Anania, and D. Sharma
Leptin-induced Growth Stimulation of Breast Cancer Cells Involves Recruitment of Histone Acetyltransferases and Mediator Complex to CYCLIN D1 Promoter via Activation of Stat3
J. Biol. Chem., May 4, 2007; 282(18): 13316 - 13325.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
P Kauraniemi and A Kallioniemi
Activation of multiple cancer-associated genes at the ERBB2 amplicon in breast cancer.
Endocr. Relat. Cancer, March 1, 2006; 13(1): 39 - 49.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. K. Pandey, T. S. Udayakumar, X. Lin, D. Sharma, P. S. Shapiro, and J. D. Fondell
Activation of TRAP/Mediator Subunit TRAP220/Med1 Is Regulated by Mitogen-Activated Protein Kinase-Dependent Phosphorylation
Mol. Cell. Biol., December 15, 2005; 25(24): 10695 - 10710.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
K D S. A. Wansa and G. E O Muscat
TRAP220 is modulated by the antineoplastic agent 6-Mercaptopurine, and mediates the activation of the NR4A subgroup of nuclear receptors
J. Mol. Endocrinol., June 1, 2005; 34(3): 835 - 848.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. Rousseaux, B. Lefebvre, L. Dubuquoy, P. Lefebvre, O. Romano, J. Auwerx, D. Metzger, W. Wahli, B. Desvergne, G. C. Naccari, et al.
Intestinal antiinflammatory effect of 5-aminosalicylic acid is dependent on peroxisome proliferator-activated receptor-{gamma}
J. Exp. Med., April 18, 2005; 201(8): 1205 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. Efrati, J. Arsentiev-Rozenfeld, and I. Zelikovic
The human paracellin-1 gene (hPCLN-1): renal epithelial cell-specific expression and regulation
Am J Physiol Renal Physiol, February 1, 2005; 288(2): F272 - F283.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
M T Rae, D Niven, A Ross, T Forster, R Lathe, H O D Critchley, P Ghazal, and S G Hillier
Steroid signalling in human ovarian surface epithelial cells: the response to interleukin-1{alpha} determined by microarray analysis
J. Endocrinol., October 1, 2004; 183(1): 19 - 28.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Malik, M. Guermah, C.-X. Yuan, W. Wu, S. Yamamura, and R. G. Roeder
Structural and Functional Organization of TRAP220, the TRAP/Mediator Subunit That Is Targeted by Nuclear Receptors
Mol. Cell. Biol., September 15, 2004; 24(18): 8244 - 8254.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. K. Reddy
Peroxisome Proliferators and Peroxisome Proliferator-Activated Receptor {alpha}: Biotic and Xenobiotic Sensing
Am. J. Pathol., June 1, 2004; 164(6): 2305 - 2321.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Shen, V. Hebbar, S. Nair, C. Xu, W. Li, W. Lin, Y.-S. Keum, J. Han, M. A. Gallo, and A.-N. T. Kong
Regulation of Nrf2 Transactivation Domain Activity: THE DIFFERENTIAL EFFECTS OF MITOGEN-ACTIVATED PROTEIN KINASE CASCADES AND SYNERGISTIC STIMULATORY EFFECT OF Raf AND CREB-BINDING PROTEIN
J. Biol. Chem., May 28, 2004; 279(22): 23052 - 23060.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.