JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M209316200 on October 22, 2002

J. Biol. Chem., Vol. 277, Issue 51, 50062-50068, December 20, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/51/50062    most recent
M209316200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, C.
Right arrow Articles by Chen, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, C.
Right arrow Articles by Chen, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

GCMa Regulates the Syncytin-mediated Trophoblastic Fusion*

Chenchou YuDagger §, Kuofeng Shen§, Meiyao LinDagger , Porchun Chen, Chenchen Lin, Geen-Dong Chang, and Hungwen ChenDagger ||

From the Dagger  Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan and the  Graduate Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan

The human placental trophoblast cell can be classified as either a cytotrophoblast or a syncytiotrophoblast. Cytotrophoblasts can function as stem cells for the development of the syncytiotrophoblast layer via cell fusion. An envelope gene of the human endogenous retrovirus family W (HERV-W) called syncytin is specifically expressed in the syncytiotrophoblast layer. Syncytin is a fusogenic membrane protein; therefore, it can mediate the fusion of cytotrophoblasts into the syncytiotrophoblast layer, which is essential for pregnancy maintenance. GCMa is a placenta-specific transcription factor and is required for placental development. To study the placenta-specific fusion mediated by syncytin, we tested whether GCMa is involved in this process by regulating syncytin gene expression. In this report, we demonstrate that GCMa was able to regulate syncytin gene expression via two GCMa-binding sites upstream of the 5'-long terminal repeat of the syncytin-harboring HERV-W family member in BeWo and JEG3 cells but not in HeLa cells. Furthermore, adenovirus-directed expression of GCMa enhanced syncytin gene expression and syncytin-mediated cell fusion in BeWo and JEG3 cells but not in HeLa cells. Therefore, the integration site of the syncytin-harboring HERV-W family member in the human genome is close to the functional GCMa-binding sites by which GCMa can specifically transactivate syncytin gene expression in trophoblast cells. Our results may help to explain the mechanism underlying the cell fusion event specific for syncytiotrophoblast formation.

* This work was supported by grants from the National Science Council (91-2311-B-001-043) and Academia Sinica, Taiwan (to H. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

|| To whom correspondence should be addressed. Tel.: 011-886-2-27855696 ext. 6090; Fax: 011-886-2-27889759; E-mail: hwchen@gate.sinica.edu.tw.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. W. Schubert, A. Abendroth, K. Kilian, T. Vogler, B. Mayr, I. Knerr, and S. Hashemolhosseini
bZIP-Type transcription factors CREB and OASIS bind and stimulate the promoter of the mammalian transcription factor GCMa/Gcm1 in trophoblast cells
Nucleic Acids Res., June 1, 2008; 36(11): 3834 - 3846.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Chang, D. Mukherjea, R. M. Gobble, K. A. Groesch, R. J. Torry, and D. S. Torry
Glial Cell Missing 1 Regulates Placental Growth Factor (PGF) Gene Transcription in Human Trophoblast
Biol Reprod, May 1, 2008; 78(5): 841 - 851.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Schubert, N. Lamoureux, K. Kilian, L. Klein-Hitpass, and S. Hashemolhosseini
Identification of Integrin-{alpha}4, Rb1, and Syncytin A as Murine Placental Target Genes of the Transcription Factor GCMa/Gcm1
J. Biol. Chem., February 29, 2008; 283(9): 5460 - 5465.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-C. Chou, C. Chang, J.-H. Liu, L.-F. Chen, C.-D. Hsiao, and H. Chen
Small Ubiquitin-like Modifier Modification Regulates the DNA Binding Activity of Glial Cell Missing Drosophila Homolog a
J. Biol. Chem., September 14, 2007; 282(37): 27239 - 27249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. U. Baumann, S. Zamudio, and N. P. Illsley
Hypoxic upregulation of glucose transporters in BeWo choriocarcinoma cells is mediated by hypoxia-inducible factor-1
Am J Physiol Cell Physiol, July 1, 2007; 293(1): C477 - C485.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H.-C. Chuang, C.-W. Chang, G.-D. Chang, T.-P. Yao, and H. Chen
Histone deacetylase 3 binds to and regulates the GCMa transcription factor
Nucleic Acids Res., March 9, 2006; 34(5): 1459 - 1469.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C.-W. Chang, H.-C. Chuang, C. Yu, T.-P. Yao, and H. Chen
Stimulation of GCMa Transcriptional Activity by Cyclic AMP/Protein Kinase A Signaling Is Attributed to CBP-Mediated Acetylation of GCMa
Mol. Cell. Biol., October 1, 2005; 25(19): 8401 - 8414.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y.-H. Cheng and S. Handwerger
A Placenta-Specific Enhancer of the Human Syncytin Gene
Biol Reprod, September 1, 2005; 73(3): 500 - 509.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-S. Yang, C. Yu, H.-C. Chuang, C.-W. Chang, G.-D. Chang, T.-P. Yao, and H. Chen
FBW2 Targets GCMa to the Ubiquitin-Proteasome Degradation System
J. Biol. Chem., March 18, 2005; 280(11): 10083 - 10090.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Dupressoir, G. Marceau, C. Vernochet, L. Benit, C. Kanellopoulos, V. Sapin, and T. Heidmann
Syncytin-A and syncytin-B, two fusogenic placenta-specific murine envelope genes of retroviral origin conserved in Muridae
PNAS, January 18, 2005; 102(3): 725 - 730.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Schubert, E. Kardash, M. A. Khan, T. Cheusova, K. Kilian, M. Wegner, and S. Hashemolhosseini
Interaction, Cooperative Promoter Modulation, and Renal Colocalization of GCMa and Pitx2
J. Biol. Chem., November 26, 2004; 279(48): 50358 - 50365.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Prudhomme, G. Oriol, and F. Mallet
A Retroviral Promoter and a Cellular Enhancer Define a Bipartite Element Which Controls env ERVWE1 Placental Expression
J. Virol., November 15, 2004; 78(22): 12157 - 12168.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
A.J.G. Potgens, S. Drewlo, M. Kokozidou, and P. Kaufmann
Syncytin: the major regulator of trophoblast fusion? Recent developments and hypotheses on its action
Hum. Reprod. Update, November 1, 2004; 10(6): 487 - 496.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Hashemolhosseini and M. Wegner
Impacts of a new transcription factor family: mammalian GCM proteins in health and disease
J. Cell Biol., September 13, 2004; 166(6): 765 - 768.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
I. Knerr, B. Huppertz, C. Weigel, J. Dotsch, C. Wich, R.L. Schild, M.W. Beckmann, and W. Rascher
Endogenous retroviral syncytin: compilation of experimental research on syncytin and its possible role in normal and disturbed human placentogenesis
Mol. Hum. Reprod., August 1, 2004; 10(8): 581 - 588.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y.-H. Cheng, B. D. Richardson, M. A. Hubert, and S. Handwerger
Isolation and Characterization of the Human Syncytin Gene Promoter
Biol Reprod, March 1, 2004; 70(3): 694 - 701.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
W. C. Okulicz and C. I. Ace
Temporal Regulation of Gene Expression During the Expected Window of Receptivity in the Rhesus Monkey Endometrium
Biol Reprod, November 1, 2003; 69(5): 1593 - 1599.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J.-R. Landry and D. L. Mager
Functional Analysis of the Endogenous Retroviral Promoter of the Human Endothelin B Receptor Gene
J. Virol., July 1, 2003; 77(13): 7459 - 7466.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.