JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208848200 on October 23, 2002

J. Biol. Chem., Vol. 277, Issue 52, 50463-50468, December 27, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/52/50463    most recent
M208848200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baron, A.
Right arrow Articles by Lazdunski, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baron, A.
Right arrow Articles by Lazdunski, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Protein Kinase C Stimulates the Acid-sensing Ion Channel ASIC2a via the PDZ Domain-containing Protein PICK1*

Anne BaronDagger , Emmanuel DevalDagger , Miguel Salinas, Eric Lingueglia, Nicolas Voilley, and Michel Lazdunski§

From the Institut de Pharmacologie Moléculaire et Cellulaire, CNRS-UMR6097, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France

Acid-sensing ion channels (ASICs) are cationic channels activated by extracellular protons. They are expressed in central and sensory neurons where they are involved in neuromodulation and in pain perception. Recently, the PDZ domain-containing protein PICK1 (protein interacting with C-kinase) has been shown to interact with ASIC1a and ASIC2a, raising the possibility that protein kinase C (PKC) could regulate ASICs. We now show that the amplitude of the ASIC2a current, which was only modestly increased (~+30%) by the PKC activator 1-oleyl-2-acetyl-sn-glycerol (OAG, 50 µM) in the absence of PICK1, was strongly potentiated (~+300%) in the presence of PICK1. This PICK1-dependent regulatory effect was inhibited in the presence of a PKC inhibitory peptide and required the PDZ domain of PICK1 as well as the PDZ-binding domain of ASIC2a. We have also shown the direct PICK1-dependent phosphorylation of ASIC2a by [32P]phosphate labeling and immunoprecipitation and identified a major phosphorylation site, 39TIR, on the N terminus part of ASIC2a. The OAG-induced increase in ASIC2a current amplitude did not involve any change in the unitary conductance of the ASIC2a channel, whether co-expressed with PICK1 or not. These data provide the first demonstration of a regulation of ASICs by protein kinase phosphorylation and its potentiation by the partner protein PICK1.

* This work was supported by the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), the Ministère de la Recherche (ACI, Molécules et Cibles Thérapeutiques), the Association Française contre les Myopathies (AFM), the Association pour la Recherche sur le Cancer (ARC) and the AstraZenecaAB Research Area Central Nervous System/Pain.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Both authors contributed equally to this work.

§ To whom correspondence should be addressed. Tel.: 33-4-93-95-77-02 or 03; Fax: 33-4-93-95-77-04; E-mail: ipmc@ipmc.cnrs.fr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
A. Baron, N. Voilley, M. Lazdunski, and E. Lingueglia
Acid Sensing Ion Channels in Dorsal Spinal Cord Neurons
J. Neurosci., February 6, 2008; 28(6): 1498 - 1508.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Chai, M. Li, J. Lan, Z.-G. Xiong, J. A. Saugstad, and R. P. Simon
A Kinase-anchoring Protein 150 and Calcineurin Are Involved in Regulation of Acid-sensing Ion Channels ASIC1a and ASIC2a
J. Biol. Chem., August 3, 2007; 282(31): 22668 - 22677.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Joch, A. R. Ase, C. X.-Q. Chen, P. A. MacDonald, M. Kontogiannea, A. T. Corera, A. Brice, P. Seguela, and E. A. Fon
Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels
Mol. Biol. Cell, August 1, 2007; 18(8): 3105 - 3118.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Chanrion, C. Mannoury la Cour, F. Bertaso, M. Lerner-Natoli, M. Freissmuth, M. J. Millan, J. Bockaert, and P. Marin
From the Cover: Physical interaction between the serotonin transporter and neuronal nitric oxide synthase underlies reciprocal modulation of their activity
PNAS, May 8, 2007; 104(19): 8119 - 8124.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. Yagi, H. N. Wenk, L. A. Naves, and E. W. McCleskey
Sustained Currents Through ASIC3 Ion Channels at the Modest pH Changes That Occur During Myocardial Ischemia
Circ. Res., September 1, 2006; 99(5): 501 - 509.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Wang, B. Duan, H. Xu, L. Xu, and T.-L. Xu
Calcium-permeable Acid-sensing Ion Channel Is a Molecular Target of the Neurotoxic Metal Ion Lead
J. Biol. Chem., February 3, 2006; 281(5): 2497 - 2505.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Salinas, L. D. Rash, A. Baron, G. Lambeau, P. Escoubas, and M. Lazdunski
The receptor site of the spider toxin PcTx1 on the proton-gated cation channel ASIC1a
J. Physiol., January 15, 2006; 570(2): 339 - 354.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Donier, F. Rugiero, K. Okuse, and J. N. Wood
Annexin II Light Chain p11 Promotes Functional Expression of Acid-sensing Ion Channel ASIC1a
J. Biol. Chem., November 18, 2005; 280(46): 38666 - 38672.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. L. Madsen, T. Beuming, M. Y. Niv, C.-w. Chang, K. K. Dev, H. Weinstein, and U. Gether
Molecular Determinants for the Complex Binding Specificity of the PDZ Domain in PICK1
J. Biol. Chem., May 27, 2005; 280(21): 20539 - 20548.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Price, R. J. Thompson, J. O. Eshcol, J. A. Wemmie, and C. J. Benson
Stomatin Modulates Gating of Acid-sensing Ion Channels
J. Biol. Chem., December 17, 2004; 279(51): 53886 - 53891.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Hruska-Hageman, C. J. Benson, A. S. Leonard, M. P. Price, and M. J. Welsh
PSD-95 and Lin-7b Interact with Acid-sensing Ion Channel-3 and Have Opposite Effects on H+-gated Current
J. Biol. Chem., November 5, 2004; 279(45): 46962 - 46968.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Bjerggaard, J. U. Fog, H. Hastrup, K. Madsen, C. J. Loland, J. A. Javitch, and U. Gether
Surface Targeting of the Dopamine Transporter Involves Discrete Epitopes in the Distal C Terminus But Does Not Require Canonical PDZ Domain Interactions
J. Neurosci., August 4, 2004; 24(31): 7024 - 7036.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Deval, M. Salinas, A. Baron, E. Lingueglia, and M. Lazdunski
ASIC2b-dependent Regulation of ASIC3, an Essential Acid-sensing Ion Channel Subunit in Sensory Neurons via the Partner Protein PICK-1
J. Biol. Chem., May 7, 2004; 279(19): 19531 - 19539.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. S. Leonard, O. Yermolaieva, A. Hruska-Hageman, C. C. Askwith, M. P. Price, J. A. Wemmie, and M. J. Welsh
cAMP-dependent protein kinase phosphorylation of the acid-sensing ion channel-1 regulates its binding to the protein interacting with C-kinase-1
PNAS, February 18, 2003; 100(4): 2029 - 2034.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.