HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 52, 50683-50692, December 27, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/52/50683    most recent M209221200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kusner, D. J. Articles by Iyer, S. S. Search for Related Content PubMed PubMed Citation Articles by Kusner, D. J. Articles by Iyer, S. S. Social Bookmarking                      What's this?

Regulation of Phospholipase D Activity by Actin
ACTIN EXERTS BIDIRECTIONAL MODULATION OF MAMMALIAN PHOSPOLIPASE D ACTIVITY IN A POLYMERIZATION-DEPENDENT, ISOFORM-SPECIFIC MANNER^*

David J. Kusner^§¶, James A. Barton^§, Kuo-Kuang Wen^**, Xuemin Wang, Peter A. Rubenstein^**, and Shankar S. Iyer^§

From the  Department of Internal Medicine, Division of Infectious Diseases, ^§ Inflammation Program, ^¶ Graduate Programs in Immunology and Molecular Biology, and ^** Department of Biochemistry, University of Iowa and Veterans Affairs Medical Center, Iowa City, Iowa 52242 and  Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506

Many critical cellular processes, including proliferation, vesicle trafficking, and secretion, are regulated by both phospholipase D (PLD) and the actin microfilament system. Stimulation of human PLD1 results in its association with the detergent-insoluble actin cytoskeleton, but the molecular mechanisms and functional consequences of PLD-actin interactions remain incompletely defined. Biochemical and pharmacologic modulation of actin polymerization resulted in complex bidirectional effects on PLD activity, both in vitro and in vivo. Highly purified G-actin inhibited basal and stimulated PLD activity, whereas F-actin produced the opposite effects. Actin-induced modulation of PLD activity was independent of the activating stimulus. The efficacy and potency of the effects of actin were isoform-specific but broadly conserved among actin family members. Human -actin was only 45% as potent and 40% as efficacious as rabbit skeletal muscle -actin, whereas its inhibitory profile was similar to the single actin species from the yeast, Saccharomyces cerevisiae. Use of actin polymerization-specific reagents indicated that PLD1 binds both monomeric G-actin, as well as actin filaments. These data are consistent with a model in which the physical state of the actin cytoskeleton is a critical determinant of its regulation of PLD activity.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. J. Wolyniak and P. Sundstrom Role of Actin Cytoskeletal Dynamics in Activation of the Cyclic AMP Pathway and HWP1 Gene Expression in Candida albicans Eukaryot. Cell, October 1, 2007; 6(10): 1824 - 1840. [Abstract] [Full Text] [PDF]

				D. J. Kusner, C. R. Thompson, N. A. Melrose, S. M. Pitson, L. M. Obeid, and S. S. Iyer The Localization and Activity of Sphingosine Kinase 1 Are Coordinately Regulated with Actin Cytoskeletal Dynamics in Macrophages J. Biol. Chem., August 10, 2007; 282(32): 23147 - 23162. [Abstract] [Full Text] [PDF]

				N. Lehman, B. Ledford, M. Di Fulvio, K. Frondorf, L. C. McPhail, and J. Gomez-Cambronero Phospholipase D2-derived phosphatidic acid binds to and activates ribosomal p70 S6 kinase independently of mTOR FASEB J, April 1, 2007; 21(4): 1075 - 1087. [Abstract] [Full Text] [PDF]

				G. Li and H.-W. Xue Arabidopsis PLD{zeta}2 Regulates Vesicle Trafficking and Is Required for Auxin Response PLANT CELL, January 1, 2007; 19(1): 281 - 295. [Abstract] [Full Text] [PDF]

				S. Huang, L. Gao, L. Blanchoin, and C. J. Staiger Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid Mol. Biol. Cell, April 1, 2006; 17(4): 1946 - 1958. [Abstract] [Full Text] [PDF]

				S. S. Iyer, R. S. Agrawal, C. R. Thompson, S. Thompson, J. A. Barton, and D. J. Kusner Phospholipase D1 Regulates Phagocyte Adhesion J. Immunol., March 15, 2006; 176(6): 3686 - 3696. [Abstract] [Full Text] [PDF]

				O. Diaz, S. Mebarek-Azzam, A. Benzaria, M. Dubois, M. Lagarde, G. Nemoz, and A.-F. Prigent Disruption of Lipid Rafts Stimulates Phospholipase D Activity in Human Lymphocytes: Implication in the Regulation of Immune Function J. Immunol., December 15, 2005; 175(12): 8077 - 8086. [Abstract] [Full Text] [PDF]

				J. Ziembicki, R. Tandon, J. R. Schelling, J. R. Sedor, R. T. Miller, and C. Huang Mechanical force-activated phospholipase D is mediated by G{alpha}12/13-Rho and calmodulin-dependent kinase in renal epithelial cells Am J Physiol Renal Physiol, October 1, 2005; 289(4): F826 - F834. [Abstract] [Full Text] [PDF]

				H. Komati, F. Naro, S. Mebarek, V. De Arcangelis, S. Adamo, M. Lagarde, A.-F. Prigent, and G. Nemoz Phospholipase D Is Involved in Myogenic Differentiation through Remodeling of Actin Cytoskeleton Mol. Biol. Cell, March 1, 2005; 16(3): 1232 - 1244. [Abstract] [Full Text] [PDF]

				S. S. Iyer, J. A. Barton, S. Bourgoin{section}, and D. J. Kusner Phospholipases D1 and D2 Coordinately Regulate Macrophage Phagocytosis J. Immunol., August 15, 2004; 173(4): 2615 - 2623. [Abstract] [Full Text] [PDF]

				J. L. Edwards, D. D. Entz, and M. A. Apicella Gonococcal Phospholipase D Modulates the Expression and Function of Complement Receptor 3 in Primary Cervical Epithelial Cells Infect. Immun., November 1, 2003; 71(11): 6381 - 6391. [Abstract] [Full Text] [PDF]